INT165054

Context Info Confidence 0.58 First Reported 2007 Last Reported 2010 Negated 0 Speculated 4 Reported most in Body Documents 45 Total Number 85 Disease Relevance 82.87 Pain Relevance 28.82

Anatomy Link Frequency T cells 35 synovium 13 blood 2 joint 2 thyroid 2 MIR146A (Homo sapiens)

Pain Link Frequency Relevance Heat Osteoarthritis 975 99.92 rheumatoid arthritis 5099 99.88 cytokine 750 99.84 Inflammation 1027 99.64 psoriasis 86 94.72 Multiple sclerosis 78 88.96 tolerance 43 83.08 methotrexate 39 60.56 cINOD 121 59.44 corticosteroid 43 56.32

Disease Link Frequency Relevance Heat Osteoarthritis 936 99.92 Rheumatoid Arthritis 5099 99.88 INFLAMMATION 1066 99.64 Thyroid Neoplasm 12 99.58 Disease 1408 99.56 Apoptosis 1088 99.36 Hyperplasia 78 99.16 Systemic Lupus Erythematosus 86 98.56 Cancer 465 98.32 Necrosis 125 98.08

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Susceptibility to Chlamydia trachomatis infections is 40% host based. microRNA-146a is a negative regulator of Tolllike receptor (TLR) signaling and possesses functional polymorphisms which decrease the production of premiR-146a and mature miR-146a.

Gene_expression (production) of miR-146a associated with chlamydia infection and infection

1) Confidence 0.58 Published 2009 Journal Drugs Today Section Abstract Doc Link 20011700 Disease Relevance 0.55 Pain Relevance 0

He et al. also showed that miR-146, miR-221 and miR-222 distinguished unequivocally between papillary thyroid carcinoma (PTC) and normal thyroid.

Gene_expression (distinguished) of miR-146 in thyroid associated with thyroid neoplasm

2) Confidence 0.45 Published 2007 Journal Mol Cancer Section Body Doc Link PMC2098778 Disease Relevance 0.58 Pain Relevance 0

MiR-150 has not been associated with the high expression of miR-146 and miR-155 in PBMC and synovium of RA in previous reports [24-26].

Gene_expression (expression) of miR-146 in synovium associated with rheumatoid arthritis

3) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 0.73 Pain Relevance 0.17

In a previous report, accumulated CD3+ T cells were observed to express miR-146, thereby suggesting that miR-146 might play a role in the persistent inflammation in RA via a T cell network, which strongly supports that miR-146a thus expressed in Th17 cells [25].

Gene_expression (expressed) of miR-146a in Th17 cells associated with inflammation and rheumatoid arthritis

4) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.03 Pain Relevance 0.37

Indeed, previous reports demonstrated that miR-146a/b, miR-150 and miR-155 are expressed in PBMC or synovium in RA patients, which might include miRNAs which are expressed in IL-17 producing T cells.

Gene_expression (expressed) of miR-146a in synovium associated with rheumatoid arthritis

5) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 0.91 Pain Relevance 0.31

The expression level of miR-146a was similar to that of IL-17.

Gene_expression (expression) of miR-146a

6) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.54 Pain Relevance 0.71

The analysis of the synovium from RA and OA patients revealed that miR-146a/b, miR-150 and miR-155 were highly expressed in RA synovium with hyperplasia and infiltration of inflammatory cells including Th17 producing cells in poorly controlled patients with severe joint destruction (Figure 4A).

Gene_expression (expressed) of miR-146a in synovium associated with inflammation, rheumatoid arthritis, osteoarthritis and hyperplasia

7) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.45 Pain Relevance 0.58

Therefore, the expression pattern of miR-146a/b, miR-150, and miR-155 in the synovium of RA patients was examined using real time PCR. miR-146a, miR-146b, miR-150, and miR-155 were expressed intensely in RA in comparison to OA.

Spec (examined) Gene_expression (expression) of miR-146a in synovium associated with rheumatoid arthritis and osteoarthritis

8) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.71 Pain Relevance 0.75

The synovium with high expression of miR-146a, miR-146b, miR-150 and miR-155 showed vigorous proliferation of synovial cells and infiltration of inflammatory cells and abundant IL-17 positive cells.

Gene_expression (expression) of miR-146a in synovium associated with inflammation

9) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.56 Pain Relevance 0.61

In a previous report, accumulated CD3+ T cells were observed to express miR-146, thereby suggesting that miR-146 might play a role in the persistent inflammation in RA via a T cell network, which strongly supports that miR-146a thus expressed in Th17 cells [25].

Gene_expression (express) of miR-146 in Th17 cells associated with inflammation and rheumatoid arthritis

10) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.12 Pain Relevance 0.35

The expression of miR-146a and miR-146b tended to increase with high disease activity, which was consistent with previous reports[24,25].

Gene_expression (expression) of miR-146a associated with disease

11) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.56 Pain Relevance 0.63

There were significant differences between RA and OA patients in the expression level of miR-146a, miR-150 and miR-155.

Gene_expression (expression) of miR-146a associated with rheumatoid arthritis and osteoarthritis

12) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.21 Pain Relevance 0.57

MiR-146a was expressed intensely with IL-17 expression in PBMC from the patients with early stage of RA and high disease activity. miR-146a/b was associated with high disease activity, and miR-150 was intensely expressed in the patients with severe joint destruction.

Gene_expression (expressed) of MiR-146a in joint associated with rheumatoid arthritis and disease

13) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 0.48 Pain Relevance 0.12

The expression level of miR-26a, miR-146a/b, miR-150 and miR-155 in RA patients were significantly higher than in healthy subjects.

Gene_expression (expression) of miR-146a associated with rheumatoid arthritis

14) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.09 Pain Relevance 0.54

In addition, the miR-146a expression in the IL-17 producing T cells of the RA synovium was confirmed.

Gene_expression (expression) of miR-146a in synovium associated with rheumatoid arthritis

15) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.07 Pain Relevance 0.35

The expression of miR-146a and IL-17 was high in PBMC in patients with low score of Larsen grade and short disease duration in the current series.

Gene_expression (expression) of miR-146a associated with disease

16) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 0.77 Pain Relevance 0.17

Therefore, the high expression of miR-146a in PBMC was strongly associated with IL-17 expression, especially at the early stage of RA.

Gene_expression (expression) of miR-146a associated with rheumatoid arthritis

17) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 0.84 Pain Relevance 0.17

The synovium from RA patients exhibit increased expression of miR-146a,b, 150, and 155

Gene_expression (expression) of miR-146a in synovium associated with rheumatoid arthritis

18) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.75 Pain Relevance 0.80

The expression level of miR-146a and IL-17 in patient RA1 and RA4 was high in comparison to the other patients. miR-146a expressed intensely with IL-17 expression in PBMC from the patients with early stage of RA and high disease activity (Figure 2B).

Gene_expression (expression) of miR-146a associated with rheumatoid arthritis and disease

19) Confidence 0.39 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.60 Pain Relevance 0.74

The expression level of miR-146a and IL-17 in patient RA1 and RA4 was high in comparison to the other patients. miR-146a expressed intensely with IL-17 expression in PBMC from the patients with early stage of RA and high disease activity (Figure 2B).

Gene_expression (expressed) of miR-146a associated with rheumatoid arthritis and disease

20) Confidence 0.34 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC2950393 Disease Relevance 1.70 Pain Relevance 0.79

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