INT166377

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Context Info
Confidence 0.68
First Reported 2009
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 3
Total Number 17
Disease Relevance 2.90
Pain Relevance 0.97

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

mitochondrion (Pick1) protein targeting (Pick1) Golgi apparatus (Pick1)
ATPase activity (Pick1) plasma membrane (Pick1) enzyme binding (Pick1)
Anatomy Link Frequency
Mouse 1
upper 1
frontal cortex 1
Pick1 (Mus musculus)
Pain Link Frequency Relevance Heat
Morphine 9 98.82 Very High Very High Very High
Hippocampus 3 98.56 Very High Very High Very High
Nucleus accumbens 3 97.92 Very High Very High Very High
Dopamine 30 97.44 Very High Very High Very High
Glutamate receptor 19 89.92 High High
nMDA receptor 22 87.36 High High
ASIC 165 78.52 Quite High
Glutamate 17 70.96 Quite High
long-term potentiation 84 58.76 Quite High
imagery 45 51.60 Quite High
Disease Link Frequency Relevance Heat
Urological Neuroanatomy 3 100.00 Very High Very High Very High
Toxicity 90 99.80 Very High Very High Very High
Death 120 99.08 Very High Very High Very High
Acidosis 60 98.92 Very High Very High Very High
Apoptosis 15 87.20 High High
Disease 60 86.84 High High
Targeted Disruption 16 63.44 Quite High
Brain Injury 15 37.52 Quite Low
Male Infertility 15 32.96 Quite Low
Pain 22 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
We transfected ASIC1a, with or without PICK1, into COS7 cells.
Gene_expression (transfected) of PICK1
1) Confidence 0.68 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.54 Pain Relevance 0
Co-expressing of PICK1 with mouse ASIC1a also increased the population of faster migrating ASIC1a (Figure 1D).
Gene_expression (expressing) of PICK1
2) Confidence 0.68 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0
ASIC1a and myc-PICK1 were transfected into COS7 cells using lipofectamine 2000 (Invitrogen).
Gene_expression (transfected) of PICK1
3) Confidence 0.68 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0
PNGase treatment reduced all slower migrating species to the faster migrating species in both control and PICK1 overexpressing conditions.
Gene_expression (overexpressing) of PICK1
4) Confidence 0.68 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0
In contrast to wild-type PICK1, when PICK 2K-E mutant was co-transfected with ASIC1a in 293T cells, we did not observe any cluster formation (Figure 2C).
Gene_expression (transfected) of PICK
5) Confidence 0.65 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0
As expected, when co-expressed with ASIC1a, both PICK1 2K-E (ASIC1a + PICK1 2K-E: 126.9% ± 7.8%, 141.5% ± 10.7%, 164.0% ± 9.6% for 2, 4, 6 hours respectively.
Gene_expression (expressed) of PICK1
6) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.35 Pain Relevance 0
As expected, PICK1 KD-AA had no effect on ASIC1a surface levels (surface ASIC1a ratio with PICK1 KA-AA: 5.42% ± 1.6%, p < 0.05 comparing to ASIC1a + PICK1, not significant comparing to ASIC1a alone or ASIC1a + PICK1 2K-E, n = 4, Figure 1C).
Gene_expression (ratio) of PICK1
7) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0.04
Mouse ASIC1a was co-transfected with eGFP or GFP-PICK1.
Gene_expression (transfected) of PICK1 in Mouse
8) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.17 Pain Relevance 0.03
In contrast, overexpression of PICK1 decreases surface expression of AMPA receptors [31,33,36,51] and netrin-1 receptor UNC5H1 [52].
Gene_expression (overexpression) of PICK1
9) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.06 Pain Relevance 0.08
When ASIC1a and wild-type PICK1 were co-transfected into 293T cells, they formed co-clusters in the perinuclear region (Figure 2B, upper panel).
Gene_expression (transfected) of PICK1 in upper
10) Confidence 0.53 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0
Co-expression of PICK1 with ASIC1a further increased acidosis-induced cell death (ASIC1a + PICK1 transfected cells: 155.0% ± 11.4%, 192.0% ± 9.0%, 244.0% ± 16.6% for 2, 4, 6 hours respectively. n = 10, ** p < 0.01, *** p < 0.001 compared to control groups; * p < 0.05 when compared with ASIC1a-transfected cells, Figure 4).
Gene_expression (-) of PICK1 associated with acidosis and death
11) Confidence 0.53 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.62 Pain Relevance 0
Here, we show that PICK1 overexpression increases ASIC1a surface level.
Gene_expression (overexpression) of PICK1
12) Confidence 0.53 Published 2010 Journal Mol Brain Section Abstract Doc Link PMC3018362 Disease Relevance 0.17 Pain Relevance 0.04
ASIC1a or ASIC1a with PICK1 were transfected into HEK293T cells.
Gene_expression (transfected) of PICK1
13) Confidence 0.53 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.17 Pain Relevance 0
Co-expression of PICK1 with ASIC1a further increased acidosis-induced cell death (ASIC1a + PICK1 transfected cells: 155.0% ± 11.4%, 192.0% ± 9.0%, 244.0% ± 16.6% for 2, 4, 6 hours respectively. n = 10, ** p < 0.01, *** p < 0.001 compared to control groups; * p < 0.05 when compared with ASIC1a-transfected cells, Figure 4).
Gene_expression (expression) of PICK1 associated with acidosis and death
14) Confidence 0.53 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.62 Pain Relevance 0
We co-transfected ASIC1a with wild-type PICK1 or BAR-domain mutant PICK1 2K-E into HEK293T cells.
Gene_expression (transfected) of PICK1
15) Confidence 0.51 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0.03
Interaction between EphB receptors and GRIP or PICK-1 may instead affect membrane trafficking of the GluR2 subunit of AMPA receptors [48].
Gene_expression (receptors) of PICK-1
16) Confidence 0.24 Published 2009 Journal Mol Pain Section Body Doc Link PMC2704201 Disease Relevance 0 Pain Relevance 0.24
Morphine reexposure induces the decrease of receptor for activated C-kinase 1 protein (RACK1) levels in frontal cortex, and the increase of p-ERK (extracellular signal-regulated kinase) levels in mouse frontal cortex, striatum, hippocampus and nucleus accumbens (NAcc).
Gene_expression (levels) of C-kinase 1 protein in frontal cortex associated with nucleus accumbens, urological neuroanatomy, hippocampus and morphine
17) Confidence 0.20 Published 2009 Journal Arch Ital Biol Section Abstract Doc Link 20162862 Disease Relevance 0.20 Pain Relevance 0.52

General Comments

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