INT168959

Context Info Confidence 0.65 First Reported 2008 Last Reported 2010 Negated 0 Speculated 0 Reported most in Abstract Documents 5 Total Number 5 Disease Relevance 0.70 Pain Relevance 2.20

cell differentiation (CAV3) Golgi apparatus (CAV3) cytoplasm (CAV3)

Anatomy Link Frequency muscle 2 muscle cells 1 CAV3 (Homo sapiens)

Pain Link Frequency Relevance Heat Cholecystokinin 52 100.00 opioid receptor 4 93.20 Enkephalin 4 91.60 cytokine 5 71.40 metalloproteinase 6 60.64 Inflammation 3 45.92 backache 8 5.00 imagery 2 5.00 Osteoarthritis 1 5.00

Disease Link Frequency Relevance Heat Aging 62 81.88 Stress 15 80.48 Adenocarcinoma 1 64.32 INFLAMMATION 3 45.92 Osteoarthritis 3 27.96 Atherosclerotic Plaque 1 25.88 Low Back Pain 8 5.00 Myeloid Leukemia 3 5.00 Retinoblastoma 1 5.00 Targeted Disruption 1 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Contraction was measured in muscle cells transfected with CAV3 mRNA or clathrin heavy-chain small-interfering RNA (siRNA).

Gene_expression (transfected) of CAV3 in muscle cells

1) Confidence 0.65 Published 2010 Journal Am. J. Physiol. Gastrointest. Liver Physiol. Section Abstract Doc Link 20558763 Disease Relevance 0 Pain Relevance 0.40

CAV-3 siRNA reduced CAV-3 protein expression, decreased CCK-8-induced contraction, and accumulated CCK-1R in the caveolae.

Gene_expression (expression) of CAV-3 protein associated with cholecystokinin

2) Confidence 0.62 Published 2010 Journal Am. J. Physiol. Gastrointest. Liver Physiol. Section Abstract Doc Link 20558763 Disease Relevance 0 Pain Relevance 0.63

We found that impaired muscle contraction in GBs with cholesterol stones is due to high caveolar levels of cholesterol that inhibits pCAV-3 generation.

Gene_expression (generation) of pCAV-3 in muscle

3) Confidence 0.42 Published 2010 Journal Am. J. Physiol. Gastrointest. Liver Physiol. Section Abstract Doc Link 20558763 Disease Relevance 0 Pain Relevance 0.48

Pretreatment with cholesterol-rich liposomes inhibited the transfer of CCK-1R and of CAV-3 in the endosomes by blocking CAV-3 phosphorylation. 4-Amino-5-(4-chloro-phenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (inhibitor of tyrosine kinase) reproduced these effects by blocking pCAV-3 formation, increasing CAV-3 and CCK-1R sequestration in the caveolae and impairing CCK-8-induced contraction.

Gene_expression (transfer) of CAV-3 associated with cholecystokinin

4) Confidence 0.42 Published 2010 Journal Am. J. Physiol. Gastrointest. Liver Physiol. Section Abstract Doc Link 20558763 Disease Relevance 0 Pain Relevance 0.57

Expression of caveolin-3 is muscle-specific, whereas caveolin-1 and caveolin-2 are coexpressed in many cell types [26].

Gene_expression (Expression) of caveolin-3 in muscle

5) Confidence 0.18 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2575636 Disease Relevance 0.70 Pain Relevance 0.12

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