INT168975

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Context Info
Confidence 0.43
First Reported 2009
Last Reported 2010
Negated 1
Speculated 0
Reported most in Body
Documents 14
Total Number 15
Disease Relevance 7.37
Pain Relevance 0.07

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleoplasm (Wt1) RNA binding (Wt1) nucleus (Wt1)
intracellular (Wt1) DNA binding (Wt1) cytoplasm (Wt1)
Anatomy Link Frequency
podocyte 2
neck 1
head 1
Wt1 (Mus musculus)
Pain Link Frequency Relevance Heat
Angina 1 65.44 Quite High
withdrawal 24 5.00 Very Low Very Low Very Low
member 8 12 5.00 Very Low Very Low Very Low
Pain 2 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Cancer 67 100.00 Very High Very High Very High
Hematologic Neoplasms 4 96.88 Very High Very High Very High
Epithelioid Hemangioendothelioma 2 96.08 Very High Very High Very High
Chronic Myeloid Leukemia 4 95.00 High High
Renal Disease 72 94.20 High High
Precursor Cell Lymphoblastic Leukemia-lymphoma 2 93.80 High High
Myelodysplastic Syndromes 14 92.56 High High
Renal Failure 36 91.24 High High
Solid Tumor 2 90.80 High High
Proteinuria 24 90.48 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The unexpected finding that the identified transcripts were not associated with WT1(+KTS) prompted us to attempt to uncover a plausible explanation to account for the identification of this subset of mRNAs encoding a remarkable functionally interrelated set of proteins.
WT1 Binding (associated) of
1) Confidence 0.43 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0 Pain Relevance 0
An underlying hypothesis was that the proteins encoded by mRNAs bound by WT1(+KTS) might define a “post-transcriptional operon” that is important for maintenance of podocyte morphology and function [26].
WT1 Binding (bound) of in podocyte
2) Confidence 0.43 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0.13 Pain Relevance 0
These results were highly reproducible and thus the enrichment of Myh10 in the pulldowns using empty vector control extracts clearly indicated that the enrichment of this mRNA did not require an association with WT1(+KTS).
WT1 Neg (not) Binding (association) of
3) Confidence 0.43 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0 Pain Relevance 0
We originally sought to identify mRNAs associated with WT1(+KTS) through an RNA immunoprecipitation and microarray approach, hypothesizing that the proteins encoded by these mRNAs might be important for podocyte morphology and function.
WT1 Binding (associated) of in podocyte
4) Confidence 0.43 Published 2009 Journal PLoS ONE Section Abstract Doc Link PMC2714980 Disease Relevance 0.43 Pain Relevance 0
Although the co-purified mRNAs were not enriched due to an association with WT1(+KTS), the significant examples of their common functions in the actin cytoskeleton raised the question as to how or why they were specifically enriched in the pull down assays.
WT1 Binding (association) of
5) Confidence 0.43 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0 Pain Relevance 0
Thus, despite the identification of a previously described WT1(+KTS) target mRNA in the results and the remarkable physiologically relevant functions of the encoded proteins, these results established that their identification was not due to WT1(+KTS) binding as envisioned by the experimental design.


WT1 Binding (binding) of
6) Confidence 0.43 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0 Pain Relevance 0
Although the Wilms' tumor 1 (WT1) gene was first isolated as a tumor suppressor gene associated with the Wilms' tumor, the WT1 gene has been shown to be highly expressed in hematopoietic malignancies including acute and chronic myelogenous leukemia, acute lymphocytic leukemia and myelodysplastic syndrome, and a majority of solid tumors including glioblastoma, lung cancer, breast cancer, colorectal cancer, thyroid cancer, renal cancer, bone/soft tissue sarcoma and head/neck squamous cell carcinoma 3.
WT1 Binding (associated) of in head associated with lung cancer, precursor cell lymphoblastic leukemia-lymphoma, renal cancer, colon cancer, breast cancer, hematologic neoplasms, skin cancer, sarcoma, thyroid neoplasm, cancer, glioblastoma, chronic myeloid leukemia, myelodysplastic syndromes and solid tumor
7) Confidence 0.34 Published 2010 Journal International Journal of Medical Sciences Section Body Doc Link PMC2860640 Disease Relevance 2.24 Pain Relevance 0
This observation was consistent with the hypothesis that the mRNAs that we identified were bound by and associated specifically with WT1(+KTS).
WT1 Binding (associated) of
8) Confidence 0.34 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0.25 Pain Relevance 0
Further validation experiments using qRT-PCR were carried out to more definitively determine whether the enrichment of these mRNAs was specifically due to association with WT1(+KTS).
WT1 Binding (association) of
9) Confidence 0.34 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0.11 Pain Relevance 0
However, we were surprised to find that in subsequent validations, we detemined that the enrichment these mRNAs was clearly not due to association with WT1(+KTS).
WT1 Binding (association) of
10) Confidence 0.34 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0.18 Pain Relevance 0
-actinin-1 (Actn1) is the only identified cellular mRNA shown to be bound by WT1(+KTS) [46].
WT1 Binding (bound) of
11) Confidence 0.32 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0.40 Pain Relevance 0
However, while our findings did not address the original hypothesis that WT1(+KTS) directly binds mRNAs in vivo, we nonetheless identified transcripts that encode a subset of interrelated, physiologically relevant proteins that we propose will provide further insights into actin cytoskeletal organization.


WT1 Binding (binds) of
12) Confidence 0.32 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0 Pain Relevance 0
However, these mRNAs were subsequently, and unexpectedly, shown not to be bound by WT1(+KTS).
WT1 Binding (bound) of
13) Confidence 0.32 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0 Pain Relevance 0
Although the Wilms' tumor 1 (WT1) gene was first isolated as a tumor suppressor gene associated with the Wilms' tumor, the WT1 gene has been shown to be highly expressed in hematopoietic malignancies including acute and chronic myelogenous leukemia, acute lymphocytic leukemia and myelodysplastic syndrome, and a majority of solid tumors including glioblastoma, lung cancer, breast cancer, colorectal cancer, thyroid cancer, renal cancer, bone/soft tissue sarcoma and head/neck squamous cell carcinoma 3.
WT1 Binding (associated) of in neck associated with lung cancer, precursor cell lymphoblastic leukemia-lymphoma, renal cancer, colon cancer, breast cancer, hematologic neoplasms, skin cancer, sarcoma, thyroid neoplasm, cancer, glioblastoma, chronic myeloid leukemia, myelodysplastic syndromes and solid tumor
14) Confidence 0.12 Published 2010 Journal International Journal of Medical Sciences Section Body Doc Link PMC2860640 Disease Relevance 2.24 Pain Relevance 0
Immunohistochemically, the tumor cells were positive for factor VIII-related antigen, CD31, and CD34, and negative for calretinin and WT-1.
WT-1 Binding (negative) of associated with cancer
15) Confidence 0.06 Published 2010 Journal Nihon Kokyuki Gakkai Zasshi Section Abstract Doc Link 20560442 Disease Relevance 1.39 Pain Relevance 0.07

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