INT169943

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Context Info
Confidence 0.35
First Reported 2001
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 22
Total Number 22
Disease Relevance 10.45
Pain Relevance 0.41

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleolus (Eri1) RNA binding (Eri1) rRNA binding (Eri1)
nucleus (Eri1) intracellular (Eri1) cytoplasm (Eri1)
Anatomy Link Frequency
MCF-7 1
oocytes 1
Eri1 (Mus musculus)
Pain Link Frequency Relevance Heat
Central nervous system 16 89.52 High High
anesthesia 32 88.60 High High
Sciatic nerve 32 81.56 Quite High
dorsal root ganglion 110 77.48 Quite High
medulla 12 76.32 Quite High
Peripheral nervous system 4 74.20 Quite High
dopaminergic neurodegeneration 12 68.08 Quite High
Dopamine 60 51.40 Quite High
Neurotransmitter 12 51.04 Quite High
Potency 14 46.00 Quite Low
Disease Link Frequency Relevance Heat
Targeted Disruption 793 100.00 Very High Very High Very High
Cold Sores 272 99.98 Very High Very High Very High
Obesity 12 99.92 Very High Very High Very High
Death 105 98.72 Very High Very High Very High
Embryonic Lethality 12 98.24 Very High Very High Very High
Body Weight 42 97.88 Very High Very High Very High
Parkinson's Disease 60 96.64 Very High Very High Very High
Stress 39 95.56 Very High Very High Very High
Brain Tumor 4 95.36 Very High Very High Very High
Cytomegalovirus Infection 81 94.76 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The promoter activity was recovered after Cre-mediated excision of the stuffer sequence, and the RNAi transgene was thus expressed.
Gene_expression (expressed) of RNAi
1) Confidence 0.35 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 0.15 Pain Relevance 0
Generation of RNAi transgenic mice
Gene_expression (Generation) of RNAi associated with targeted disruption
2) Confidence 0.30 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 0.19 Pain Relevance 0
Line #8 expressed the RNAi transgene at a higher level than line #35 and was used for further experiments.
Gene_expression (expressed) of RNAi
3) Confidence 0.30 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 0.81 Pain Relevance 0
Nevertheless, the product of RNAi transgene was detected in different tissues of the double transgenic mice from line #35 as well as line #8.
Gene_expression (product) of RNAi associated with targeted disruption
4) Confidence 0.26 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 0.78 Pain Relevance 0
We applied this U6 promoter to drive the PINK1 RNAi transgene and demonstrate the absence of any leakage of the transgene expression in all tissues of the transgenic mice and a functional recovery of the promoter upon Cre induction.
Gene_expression (drive) of RNAi associated with targeted disruption
5) Confidence 0.26 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 0.62 Pain Relevance 0.05
Using transgenic RNAi approach, we generated conditional PINK1-silenced mice.
Gene_expression (approach) of RNAi associated with targeted disruption
6) Confidence 0.26 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 0.87 Pain Relevance 0
RNAi transgene product was detected by Northern blot in all tissues (Figure 2D, E).
Gene_expression (product) of RNAi
7) Confidence 0.26 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 0.78 Pain Relevance 0
In transgenic mice, an shRNA against Id4 gene induces developmental defects in kidney 12; silencing of Fgfr2 gene causes embryonic lethality 13; inhibition of Msy2 gene expression in oocytes results in reduced fertility 33; and silencing of Sod2 gene by transgenic RNAi leads to SOD2 dysfunction that results in severe consequences resembling the phenotypes of Sod2 gene knockout 14.
Gene_expression (silencing) of RNAi in oocytes associated with targeted disruption and embryonic lethality
8) Confidence 0.26 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 1.16 Pain Relevance 0
For conditional RNAi, the effective PINK1-shRNA was expressed from a Cre-loxP inducible U6 promoter which was reversibly inactivated by insertion of a loxP-flanked stuffer sequence at the TATA box 15, 18.
Gene_expression (expressed) of RNAi
9) Confidence 0.23 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 0.37 Pain Relevance 0
If an shRNA-expressing construct is integrated into the genome, it becomes a sustainable source of siRNA and produces sustained RNAi effects, an approach that is called transgenic RNAi.
Gene_expression (produces) of RNAi associated with targeted disruption
10) Confidence 0.23 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 0.33 Pain Relevance 0
Pol III promoters such as U6 are widely used to drive the expression of RNAi transgenes in animals.
Gene_expression (expression) of RNAi
11) Confidence 0.23 Published 2007 Journal International Journal of Biological Sciences Section Abstract Doc Link PMC1820878 Disease Relevance 0.66 Pain Relevance 0.03
Conditional RNAi silenced Pink1 gene expression but did not cause dopaminergic death
Gene_expression (expression) of RNAi associated with death
12) Confidence 0.23 Published 2007 Journal International Journal of Biological Sciences Section Body Doc Link PMC1820878 Disease Relevance 0.61 Pain Relevance 0.08
RNAI produces sequence-specific reduction of gene expression in various mammalian in vitro systems [6] and most recently, RNAI was shown to suppress the expression and activity of a luciferase transgene in adult mice [7], suggesting that RNAI may become a valuable molecular and possibly clinical tool for use in vivo.
Gene_expression (produces) of RNAI
13) Confidence 0.08 Published 2002 Journal BMC Neurosci Section Body Doc Link PMC134599 Disease Relevance 0.73 Pain Relevance 0
Effects of the plasmid based RNAI protocol against AGRP
Gene_expression (protocol) of RNAI
14) Confidence 0.07 Published 2002 Journal BMC Neurosci Section Body Doc Link PMC134599 Disease Relevance 0.23 Pain Relevance 0.09
It has been reported that upregulation of KIAA0128 gene expression, which has been implicated in activation of histone mRNA synthesis, was related to cell cycle arrest in MCF-7 cells after treatment with SAHA [43].
Gene_expression (synthesis) of histone mRNA in MCF-7
15) Confidence 0.04 Published 2001 Journal Breast Cancer Res Section Body Doc Link PMC13923 Disease Relevance 0.06 Pain Relevance 0
Figure 1.HSV RNAi expression systems.
Gene_expression (expression) of RNAi associated with cold sores
16) Confidence 0.02 Published 2008 Journal Nucleic Acids Research Section Body Doc Link PMC2504301 Disease Relevance 0.38 Pain Relevance 0
In cell culture, the different RNAi expression systems showed very similar silencing capabilities.
Gene_expression (expression) of RNAi
17) Confidence 0.02 Published 2008 Journal Nucleic Acids Research Section Body Doc Link PMC2504301 Disease Relevance 0.71 Pain Relevance 0.15
An RNAi-resistant Sod2 gene was generated by introducing four silent mutations within shRNA target region.
Gene_expression (generated) of RNAi
18) Confidence 0.02 Published 2006 Journal PLoS Genet Section Body Doc Link PMC1358942 Disease Relevance 0.16 Pain Relevance 0
Adenoviral vector expressing RNAi-resistant Sod2.
Gene_expression (expressing) of RNAi
19) Confidence 0.02 Published 2006 Journal PLoS Genet Section Body Doc Link PMC1358942 Disease Relevance 0.15 Pain Relevance 0
For the rescue experiments, the transgenic cells (two passages) were infected in 25-cm2 flasks with adenoviral vectors expressing RNAi-resistant Sod2 gene (see below).
Gene_expression (expressing) of RNAi associated with targeted disruption
20) Confidence 0.02 Published 2006 Journal PLoS Genet Section Body Doc Link PMC1358942 Disease Relevance 0.10 Pain Relevance 0

General Comments

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