INT170443

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Context Info
Confidence 0.12
First Reported 2001
Last Reported 2001
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 10
Disease Relevance 0.07
Pain Relevance 5.88

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

mitochondrion (Spr) nucleus (Spr) cytoplasm (Spr)
Anatomy Link Frequency
ganglia 1
Spr (Mus musculus)
Pain Link Frequency Relevance Heat
substance P 2200 100.00 Very High Very High Very High
Potency 30 70.00 Quite High
imagery 10 50.00 Quite Low
depression 10 32.16 Quite Low
Opioid 10 27.36 Quite Low
agonist 40 13.24 Low Low
Inflammation 20 5.00 Very Low Very Low Very Low
Neurotransmitter 20 5.00 Very Low Very Low Very Low
Neuropeptide 10 5.00 Very Low Very Low Very Low
opioid receptor 10 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Reprotox - General 2 20 70.00 Quite High
Depression 10 32.16 Quite Low
Tics 10 18.80 Low Low
Nociception 20 5.00 Very Low Very Low Very Low
INFLAMMATION 20 5.00 Very Low Very Low Very Low
Hypersensitivity 10 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
In order to observe the direct-labeling of the SP conjugates to the SPR, CHO cells expressing the rat SPR (rSPR), were incubated with the SP analogs (100 nM) for 2 hrs at 4°C, to prevent internalization of the receptor, rinsed and fixed.
Gene_expression (expressing) of SPR associated with substance p
1) Confidence 0.12 Published 2001 Journal BMC Chem Biol Section Body Doc Link PMC33344 Disease Relevance 0 Pain Relevance 0.47
Activation of the SPR expressed in CHO cells results in an elevation in intracellular Ca++ [7].
Gene_expression (expressed) of SPR
2) Confidence 0.12 Published 2001 Journal BMC Chem Biol Section Body Doc Link PMC33344 Disease Relevance 0 Pain Relevance 0.80
It is also of importance to determine the activity of the labeled ligands in a cell that endogenously expresses the SPR.
Gene_expression (expresses) of SPR
3) Confidence 0.10 Published 2001 Journal BMC Chem Biol Section Body Doc Link PMC33344 Disease Relevance 0 Pain Relevance 0.60
The principal neurons of bullfrog sympathetic ganglia express a SPR that has been well characterized with regard to its structure and function.
Gene_expression (express) of SPR in ganglia
4) Confidence 0.10 Published 2001 Journal BMC Chem Biol Section Body Doc Link PMC33344 Disease Relevance 0 Pain Relevance 0.58
To determine if Alexa 488-SP could specifically produce a receptor-mediated Ca++ response, the average of the fluorescence signal produced by Alexa 488-SP in the absence of cells was subtracted from the fluorescent signal obtained when Alexa 488-SP was added to SPR-expressing CHO cells.
Gene_expression (expressing) of SPR associated with substance p
5) Confidence 0.09 Published 2001 Journal BMC Chem Biol Section Body Doc Link PMC33344 Disease Relevance 0 Pain Relevance 0.59
Thus, labeling with either of these two fluorophores did not alter the functional activity of SP at the SPR in either a heterologous expression system or native cells.
Gene_expression (expression) of SPR associated with substance p
6) Confidence 0.09 Published 2001 Journal BMC Chem Biol Section Body Doc Link PMC33344 Disease Relevance 0.07 Pain Relevance 0.93
These four analogs only produced Ca++ responses in cells transfected with the SPR; they had no effect in non-transfected cells.
Gene_expression (transfected) of SPR
7) Confidence 0.09 Published 2001 Journal BMC Chem Biol Section Body Doc Link PMC33344 Disease Relevance 0 Pain Relevance 0.40
The results showed that Alexa 488-SP did not activate the SPR to produce a Ca++ response.
Gene_expression (produce) of SPR associated with substance p
8) Confidence 0.09 Published 2001 Journal BMC Chem Biol Section Body Doc Link PMC33344 Disease Relevance 0 Pain Relevance 0.58
In order to observe the direct-labeling of the SP conjugates to the SPR, CHO cells expressing the rat SPR (rSPR), were incubated with the SP analogs (100 nM) for 2 hrs at 4°C, to prevent internalization of the receptor, rinsed and fixed.
Gene_expression (labeling) of SPR associated with substance p
9) Confidence 0.09 Published 2001 Journal BMC Chem Biol Section Body Doc Link PMC33344 Disease Relevance 0 Pain Relevance 0.47
Direct-labeling of the SPR with fluorescent ligands like those analyzed in this study would offer advantages over current methods of receptor labeling.
Gene_expression (labeling) of SPR
10) Confidence 0.09 Published 2001 Journal BMC Chem Biol Section Body Doc Link PMC33344 Disease Relevance 0 Pain Relevance 0.46

General Comments

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