INT170712

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Context Info
Confidence 0.77
First Reported 2001
Last Reported 2001
Negated 0
Speculated 2
Reported most in Body
Documents 1
Total Number 29
Disease Relevance 2.33
Pain Relevance 2.33

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (Siah1a) cytosol (Siah1a) nucleus (Siah1a)
cell cycle (Siah1a) ligase activity (Siah1a) cytoplasm (Siah1a)
Anatomy Link Frequency
neurons 4
tail 1
nervous system 1
Siah1a (Rattus norvegicus)
Pain Link Frequency Relevance Heat
Glutamate 348 99.80 Very High Very High Very High
Glutamate receptor 116 97.72 Very High Very High Very High
imagery 58 92.16 High High
addiction 29 82.24 Quite High
tetrodotoxin 29 32.20 Quite Low
Neuronal excitability 29 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Ganglion Cysts 174 99.62 Very High Very High Very High
Apoptosis 29 62.00 Quite High
Cancer 29 61.36 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
This inhibition was significantly reduced to 22 ± 5% (n = 6) in cells coexpressing Siah1a (p < 0.05; figure 2).
Gene_expression (coexpressing) of Siah1a
1) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0 Pain Relevance 0.22
In cells expressing mGluR5a and GFP-Siah1a-CT, inhibition was reduced to 14 ± 3 (n = 6; p < 0.05; figure 5B).
Gene_expression (expressing) of Siah1a
2) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0.05 Pain Relevance 0.09
Since there was no detectable change in cell size accompanying Siah1a expression measured electrophysiologically (data not shown), the fluorescence measurments were not normalized to total area.



Gene_expression (expression) of Siah1a
3) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0 Pain Relevance 0
In contrast to that in cells expressing mGluR5b alone, calcium current inhibition by glutamate in cells coexpressing mGluR5b and Siah1a was quite small (figure 1C).
Gene_expression (coexpressing) of Siah1a associated with glutamate
4) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0.06 Pain Relevance 0.29
The reduction in group I mGluR signaling induced by Siah1a expression may be attributed to several possible mechanisms.
Gene_expression (expression) of Siah1a
5) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0 Pain Relevance 0.06
If the results presented here can be exprapolated to physiological situations that involve group I mGluRs, Siah1a expression in the mammalian nervous system could potentially play a role in cell excitability by influencing mGluR-mediated potassium current modulation (of M-type currents and perhaps of GIRK currents), and synaptic plasticity due to the role that group I mGluRs play in these processes [2,5,38].


Gene_expression (expression) of Siah1a in nervous system
6) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0.06 Pain Relevance 0.03
To examine the role of Siah1a expression on mGluR function, SCG neurons were injected with cDNA encoding several group I mGluRs (mGluR1, 5 and their splice variants) with or without Siah1a expression.
Gene_expression (expression) of Siah1a in neurons associated with ganglion cysts
7) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0.24 Pain Relevance 0.15
In cells coexpressing Siah1a, NE produced an inhibition of 54 ± 6% (n = 11; figure 2).
Gene_expression (coexpressing) of Siah1a
8) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0 Pain Relevance 0.30
Coexpression of Siah1a caused a reduction in calcium current modulation to 12 ± 3%, as observed previously (n = 14).
Gene_expression (Coexpression) of Siah1a
9) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0 Pain Relevance 0
Inhibition was significantly reduced to 10 ± 6% (n = 5; p < 0.05) in cells coexpressing mGluR5b and Siah1a (figure 2).
Gene_expression (coexpressing) of Siah1a
10) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0.05 Pain Relevance 0.34
In similarly treated cells coexpressing Siah1a, surface expression, measured as total cell fluorescence (figure 3B), was not significantly reduced (p = 0.33) and no receptor clustering was observed (figure 3A, lower).
Gene_expression (coexpressing) of Siah1a
11) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0 Pain Relevance 0
To examine the role of Siah1a expression on mGluR function, SCG neurons were injected with cDNA encoding several group I mGluRs (mGluR1, 5 and their splice variants) with or without Siah1a expression.
Spec (examine) Gene_expression (expression) of Siah1a in neurons associated with ganglion cysts
12) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0.25 Pain Relevance 0.10
The functional role of Siah1a was assessed by examining calcium current inhibition mediated by the expressed mGluRs in the absence and presence of heterologous Siah1a expression.
Gene_expression (expression) of Siah1a
13) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0.38 Pain Relevance 0.03
Next, when CaM was coexpressed with mGluR5b and Siah1a, calcium current modulation was significantly increased (over that in cells expressing mGluR5b and Siah1a) to 25 ± 5% (n = 12; figure 6).
Gene_expression (coexpressed) of Siah1a
14) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0 Pain Relevance 0
Coexpression of Siah1a with group I mGluRs
Gene_expression (Coexpression) of Siah1a
15) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0.36 Pain Relevance 0.10
Next, when CaM was coexpressed with mGluR5b and Siah1a, calcium current modulation was significantly increased (over that in cells expressing mGluR5b and Siah1a) to 25 ± 5% (n = 12; figure 6).
Gene_expression (expressing) of Siah1a
16) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0 Pain Relevance 0
To test whether Siah1a expression altered the surface expression of group I mGluRs, the surface distribution of mGluR5a-myc was examined in the absence and presence of Siah1a.
Spec (whether) Gene_expression (expression) of Siah1a
17) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0 Pain Relevance 0
Cells expressing mGluR2 alone exhibited 57 ± 5% inhibition of calcium current in response to glutamate (n = 6), while those coexpressing Siah1a were inhibited 61 ± 2% (n = 6; figure 2).
Gene_expression (coexpressing) of Siah1a associated with glutamate
18) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0 Pain Relevance 0.12
Sympathetic neurons from the rat SCG that were made to express Siah1a with a group I mGluR attenuated mGluR-mediated calcium current modulation.
Gene_expression (express) of Siah1a in neurons associated with ganglion cysts
19) Confidence 0.77 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0.10 Pain Relevance 0.05
Coexpression of GFP-Siah1a-CT caused a significant reduction in the magnitude of calcium current modulation via mGluR5a (figure 4C,D).
Gene_expression (Coexpression) of Siah1a
20) Confidence 0.67 Published 2001 Journal BMC Neurosci Section Body Doc Link PMC58838 Disease Relevance 0.06 Pain Relevance 0.09

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