INT171924

Context Info Confidence 0.65 First Reported 2001 Last Reported 2011 Negated 3 Speculated 3 Reported most in Body Documents 60 Total Number 83 Disease Relevance 54.38 Pain Relevance 21.68

cytosol (MAPK14) nucleoplasm (MAPK14) signal transduction (MAPK14) nucleus (MAPK14) response to stress (MAPK14) cytoplasm (MAPK14)

Anatomy Link Frequency chondrocytes 10 cartilage 1 finger 1 brain 1 central nervous system 1 MAPK14 (Homo sapiens)

Pain Link Frequency Relevance Heat Osteoarthritis 3249 100.00 Inflammation 1154 100.00 cytokine 1120 100.00 Central nervous system 296 99.98 Nicotine 384 99.20 COX2 1 98.80 IPN 4 98.50 agonist 119 98.24 rheumatoid arthritis 891 97.36 Arthritis 345 95.76

Disease Link Frequency Relevance Heat Rheumatoid Arthritis 1398 100.00 INFLAMMATION 1205 100.00 Frailty 331 100.00 Stress 330 100.00 Osteoarthritis 3005 99.72 Disease Progression 122 99.40 Cold Sores 367 99.20 Cancer 720 98.64 Inflammatory Pain 4 98.50 Apoptosis 580 98.48

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

To analyze the gene expression of the p38-MAPK isoforms ?

Spec (analyze) Gene_expression (expression) of p38

1) Confidence 0.65 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0 Pain Relevance 0.09

Expression of p38-MAPK isoforms in human OA chondrocytes

Gene_expression (Expression) of p38 in chondrocytes associated with osteoarthritis

2) Confidence 0.56 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.98 Pain Relevance 0.42

-MAPK but not p38?

Gene_expression (but) of p38

3) Confidence 0.56 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 1.01 Pain Relevance 0.44

-MAPK and p38?

Gene_expression (MAPK) of p38

4) Confidence 0.56 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.68 Pain Relevance 0.37

Next, we examined which p38-MAPK isoform was activated by IL-1?

Spec (examined) Gene_expression (examined) of p38

5) Confidence 0.56 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.67 Pain Relevance 0.33

antibody (#MAB1347) was from R&D Systems (Minneapolis, MN, USA); anti-phospho-p38MAPK antibody (#9215S), anti-p38?

Gene_expression (antibody) of p38

6) Confidence 0.56 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.19 Pain Relevance 0.10

We next examined the gene expression pattern of p38-MAPK isoforms in primary human OA chondrocytes by PCR using the gene specific primers (see method section).

Spec (examined) Gene_expression (expression) of p38 in chondrocytes associated with osteoarthritis

7) Confidence 0.56 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.92 Pain Relevance 0.39

[36], however, activation of endogenously expressed p38-MAPK family members by IL-1?

Gene_expression (expressed) of p38

8) Confidence 0.56 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.63 Pain Relevance 0.31

Anti-p38?

Gene_expression (Anti) of p38

9) Confidence 0.56 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.20 Pain Relevance 0.10

In the decomposition of the interaction map, FOS appears in Module 2 only and MAPK14 catalyzes the activation of FOS.

Gene_expression (catalyzes) of MAPK14

10) Confidence 0.52 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2855702 Disease Relevance 0.48 Pain Relevance 0.17

-MAPK isoform was undetectable in human OA chondrocytes, HeLa cells and the C28-I2 chondrocytes indicating that these cell types do not express p38?

Neg (not) Gene_expression (express) of p38 in chondrocytes associated with osteoarthritis

11) Confidence 0.50 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.72 Pain Relevance 0.40

Our results show that expression of p38?

Gene_expression (expression) of p38

12) Confidence 0.50 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.89 Pain Relevance 0.38

Gene expression of p38-MAPK isoforms was measured by RT-PCR.

Gene_expression (expression) of p38

13) Confidence 0.50 Published 2010 Journal Arthritis Res Ther Section Abstract Doc Link PMC2991031 Disease Relevance 0.63 Pain Relevance 0.32

Interestingly, the expression of p38?

Gene_expression (expression) of p38

14) Confidence 0.50 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.62 Pain Relevance 0.31

Ours results show that in human OA chondrocytes expression of p38?

Gene_expression (expression) of p38 in chondrocytes associated with osteoarthritis

15) Confidence 0.50 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.54 Pain Relevance 0.27

Human OA chondrocytes expressed p38-MAPK isoforms p38?

Gene_expression (expressed) of p38 in chondrocytes associated with osteoarthritis

16) Confidence 0.50 Published 2010 Journal Arthritis Res Ther Section Abstract Doc Link PMC2991031 Disease Relevance 0.75 Pain Relevance 0.38

Human OA chondrocytes expressed p38-MAPK isoforms p38?

Gene_expression (expressed) of p38 in chondrocytes associated with osteoarthritis

17) Confidence 0.50 Published 2010 Journal Arthritis Res Ther Section Abstract Doc Link PMC2991031 Disease Relevance 0.75 Pain Relevance 0.37

proteins was readily detected but the expression of p38?

Gene_expression (expression) of p38

18) Confidence 0.50 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.53 Pain Relevance 0.27

We thus conclude that human OA chondrocytes do not express the p38?

Neg (not) Gene_expression (express) of p38 in chondrocytes associated with osteoarthritis

19) Confidence 0.50 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.68 Pain Relevance 0.34

Although the relative expression of p38-MAPK isoforms in osteoarthritis has not been fully explored, but the best-studied is p38?

Gene_expression (expression) of p38 associated with osteoarthritis

20) Confidence 0.50 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 1.13 Pain Relevance 0.60

General Comments

This test has worked.