INT172078

Context Info Confidence 0.37 First Reported 2002 Last Reported 2010 Negated 1 Speculated 3 Reported most in Body Documents 22 Total Number 25 Disease Relevance 9.88 Pain Relevance 2.27

plasma membrane (Cd3e) signal transducer activity (Cd3e)

Anatomy Link Frequency T cells 9 B lymphocytes 3 lymphocytes 1 Th1 cell 1 lamina 1 Cd3e (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 194 99.72 rheumatoid arthritis 433 98.40 cytokine 349 93.48 Infliximab 78 84.56 Potency 4 81.36 Spinal cord 27 73.24 spinal inflammation 109 61.04 Arthritis 56 60.16 chemokine 114 52.20 psoriasis 7 50.20

Disease Link Frequency Relevance Heat Influenza Virus Infection 145 100.00 Colon Cancer 25 99.76 INFLAMMATION 205 99.72 Cancer 184 98.68 Rheumatoid Arthritis 517 98.40 Disease 278 98.40 Aging 40 97.52 Viremia 4 96.40 Infection 434 96.24 Metastasis 23 91.92

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Purified CD8+CD94+T cells from two groups displayed hyporesponsiveness to stimulation with anti-CD3 mAb, whereas CD8+CD94-T cells proliferated strongly (Fig. 4B).

Localization (Purified) of CD3 in T cells

1) Confidence 0.37 Published 2008 Journal BMC Immunol Section Body Doc Link PMC2566975 Disease Relevance 0.07 Pain Relevance 0

In a second step, CD3 and PCNA were visualized with donkey anti-goat, conjugated to Alexa Fluor 594 (Molecular probes, Eugene, OR).

Localization (visualized) of CD3

2) Confidence 0.36 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2886834 Disease Relevance 0 Pain Relevance 0

In order to obtain purified spleen cell populations for the real-time RT-PCR experiments, after erythrocyte lysis, the splenocytes were appropriately stained with PE anti CD11b for the mieloyd line, PE-anti-CD3 antibody for T lymphocytes and FITC anti CD19 for B lymphocytes (Miltenyi Biotec, Bologna, Italy) and the CD11b+, CD3+ CD19+ populations were purified (purity ?

Localization (populations) of CD3 in B lymphocytes

3) Confidence 0.34 Published 2008 Journal BMC Immunol Section Body Doc Link PMC2584092 Disease Relevance 0 Pain Relevance 0.06

In order to obtain purified spleen cell populations for the real-time RT-PCR experiments, after erythrocyte lysis, the splenocytes were appropriately stained with PE anti CD11b for the mieloyd line, PE-anti-CD3 antibody for T lymphocytes and FITC anti CD19 for B lymphocytes (Miltenyi Biotec, Bologna, Italy) and the CD11b+, CD3+ CD19+ populations were purified (purity ?

Localization (stained) of CD3 in B lymphocytes

4) Confidence 0.30 Published 2008 Journal BMC Immunol Section Body Doc Link PMC2584092 Disease Relevance 0 Pain Relevance 0.06

In order to obtain purified spleen cell populations for the real-time RT-PCR experiments, after erythrocyte lysis, the splenocytes were appropriately stained with PE anti CD11b for the mieloyd line, PE-anti-CD3 antibody for T lymphocytes and FITC anti CD19 for B lymphocytes (Miltenyi Biotec, Bologna, Italy) and the CD11b+, CD3+ CD19+ populations were purified (purity ?

Localization (populations) of CD3 in B lymphocytes

5) Confidence 0.30 Published 2008 Journal BMC Immunol Section Body Doc Link PMC2584092 Disease Relevance 0 Pain Relevance 0.06

Multi colored analysis with antibodies directed against CD3, NK1.1, ??

Localization (directed) of CD3

6) Confidence 0.30 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2887844 Disease Relevance 0.44 Pain Relevance 0

Notably, multi colored analysis with antibodies directed against CD3, NK1.1, ??

Localization (directed) of CD3

7) Confidence 0.30 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2887844 Disease Relevance 0.95 Pain Relevance 0.04

To calculate the percentage of CD68, IgA and CD3, one thousand lamina propria cells per biopsy were counted.

Localization (percentage) of CD3 in lamina

8) Confidence 0.27 Published 2007 Journal BMC Gastroenterol Section Body Doc Link PMC1995200 Disease Relevance 0.17 Pain Relevance 0.03

We evaluated Th1 cell immunity in immunized mice after vaginal challenge by quantitating the number of CD3+CD4+ cells that secrete IFN?

Localization (secrete) of CD3 in Th1 cell

9) Confidence 0.26 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2671841 Disease Relevance 0.55 Pain Relevance 0.04

R-positive cells, most likely macrophages, often co-localised with CD3-positive T cells around vessels of the synovial tissue (data not shown).

Localization (localised) of CD3 in T cells

10) Confidence 0.23 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC2206344 Disease Relevance 0.71 Pain Relevance 0.32

The presence of CD3+ and CD45+ cells was used as an indicator of T cell infiltration and the prevalence of CD4+, CD8+, CD56+ cells as indicators of T helper, T cytotoxic and NK cells, respectively and CD1a was used as a marker for antigen presenting cells (APC).

Localization (presence) of CD3 in T cell

11) Confidence 0.20 Published 2009 Journal J Transl Med Section Body Doc Link PMC2714296 Disease Relevance 1.02 Pain Relevance 0

CD4+CD28null T cells were identified by morphologically cell-like structures with co-localized immunostainings of CD3, CD4 and CD244, and were manually quantified in independent analyses performed by two persons.

Localization (localized) of CD3 in T cells

12) Confidence 0.16 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC2212553 Disease Relevance 0 Pain Relevance 0

secreting CD3+CD4+CD28null T cells was analysed by flow cytometry (see above).

Spec (analysed) Localization (secreting) of CD3 in T cells

13) Confidence 0.16 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC2212553 Disease Relevance 0.23 Pain Relevance 0.04

There was markedly increased, age-independent, proliferation of CD3?

Localization (proliferation) of CD3

14) Confidence 0.13 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2996299 Disease Relevance 0.72 Pain Relevance 0

It was not possible to colocalize CXCR5 and CD3 in serial sections, so a double-label immunohistochemistry technique was developed.

Localization (colocalize) of CD3

15) Confidence 0.12 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1065316 Disease Relevance 0.46 Pain Relevance 0.23

No co-localisation was observed between CCR9 and CD20 (B lymphocytes) or CD3 (T lymphocytes) respectively in either RA or non-RA synovia (data not shown).

Localization (localisation) of CD3 in T lymphocytes associated with rheumatoid arthritis

16) Confidence 0.12 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2945064 Disease Relevance 1.00 Pain Relevance 0.53

Decreases of CD3 and CD4 and increases of CD8 cells occur in elderly individuals.

Localization (Decreases) of CD3

17) Confidence 0.10 Published 2005 Journal Immun Ageing Section Body Doc Link PMC1325257 Disease Relevance 0.27 Pain Relevance 0.07

Multiparametric flow cytometry assays were performed to determine the relative importance of the subpopulations of influenza-specific CD3+CD4+ and CD3+CD8+ T cells that produce IL-2, IFN-?

Spec (determine) Localization (importance) of CD3 in T cells associated with influenza virus infection

18) Confidence 0.10 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877091 Disease Relevance 0.75 Pain Relevance 0.05

Multiparametric flow cytometry assays were performed to determine the relative importance of the subpopulations of influenza-specific CD3+CD4+ and CD3+CD8+ T cells that produce IL-2, IFN-?

Spec (determine) Localization (importance) of CD3 in T cells associated with influenza virus infection

19) Confidence 0.10 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877091 Disease Relevance 0.75 Pain Relevance 0.05

Phenotyping of the final products showed a patient-specific prevalence of NKT CD16+CD56+CD3+ (patient #1) or of NK CD16+CD56+CD3- (patients #2 and #3) cells.

Localization (prevalence) of CD3

20) Confidence 0.08 Published 2006 Journal J Transl Med Section Body Doc Link PMC1681349 Disease Relevance 0.05 Pain Relevance 0

General Comments

This test has worked.