INT172438

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Context Info
Confidence 0.77
First Reported 2002
Last Reported 2007
Negated 1
Speculated 0
Reported most in Body
Documents 8
Total Number 19
Disease Relevance 10.95
Pain Relevance 0.27

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Golgi apparatus (Rab27b) plasma membrane (Rab27b) GTPase activity (Rab27b)
cytoplasm (Rab27b)
Anatomy Link Frequency
mast cell 4
melanocytes 1
pigment cell 1
pituitary gland 1
Rab27b (Mus musculus)
Pain Link Frequency Relevance Heat
Serotonin 72 82.56 Quite High
imagery 48 64.88 Quite High
cytokine 12 32.32 Quite Low
anesthesia 26 5.00 Very Low Very Low Very Low
Inflammatory mediators 24 5.00 Very Low Very Low Very Low
ketamine 7 5.00 Very Low Very Low Very Low
Immobilon 7 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Targeted Disruption 1232 100.00 Very High Very High Very High
Hypersensitivity 72 99.84 Very High Very High Very High
Syndrome 87 99.08 Very High Very High Very High
Asthma 24 98.52 Very High Very High Very High
Disease 52 97.88 Very High Very High Very High
Anaphylaxis 120 93.16 High High
Choroideremia 105 92.16 High High
Retina Disease 28 83.24 Quite High
Leukemia 12 79.64 Quite High
Piebaldism 26 60.60 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Mast cells represent a cell type where both Rab27a and Rab27b are expressed, providing an opportunity to determine the relative contributions of each isoform and address redundancy issues.
Gene_expression (expressed) of Rab27b in Mast cells
1) Confidence 0.77 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0.41 Pain Relevance 0.03
The results above suggested that both Rab27a and Rab27b expression was required for normal mast cell function, presumably in the control of regulated secretion.
Gene_expression (expression) of Rab27b in mast cell
2) Confidence 0.77 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0.79 Pain Relevance 0.03
Rab27a is widely expressed (24), whereas the expression of Rab27b is more restricted and detectable in platelets, the pituitary gland and the digestive tract (31–33).
Gene_expression (expression) of Rab27b in pituitary gland
3) Confidence 0.77 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0.41 Pain Relevance 0
Both Rab27a and Rab27b isoforms are expressed in bone marrow-derived mast cells (BMMC) and localize to secretory granules.
Gene_expression (expressed) of Rab27b in mast cells
4) Confidence 0.77 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Abstract Doc Link PMC2063611 Disease Relevance 0.41 Pain Relevance 0.03
Rab27b was detected with a specific antibody and Rab27a with an anti-GFP antibody.
Gene_expression (detected) of Rab27b
5) Confidence 0.77 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0 Pain Relevance 0
The partial redundancy of the Rab27 isoforms was expected given our previous studies on the pathogenesis of Griscelli syndrome where we showed that transgenic heterologous expression of Rab27b in melanocytes could compensate for melanosome clustering and coat color dilution in Rab27a KO (ash) mice (32).
Gene_expression (expression) of Rab27b in melanocytes associated with targeted disruption and syndrome
6) Confidence 0.77 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0.56 Pain Relevance 0
(n = 21) and Rab27a+/ash; Rab27b+/?
Gene_expression (/) of Rab27b
7) Confidence 0.67 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0.63 Pain Relevance 0.04
Conversely, in Rab27b KO or double KO BMMCs, the histamine-positive granules appeared more clustered (Figure 4) in a subset of BMMCs, more specifically 32% of Rab27b KO (n = 34) and 63% of double KO (n = 8) BMMCs, compared with no obvious accumulation of granules in wild-type (n = 55), Rab27a+/ash (n = 11), Rab27aash/ash (n = 36), Rab27b+/?
Gene_expression (/) of Rab27b associated with targeted disruption
8) Confidence 0.67 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0.66 Pain Relevance 0.04
The identification of severely reduced mast cell responses in vivo in the Rab27b KO mouse is the first report that Rab27b contributes to allergic responses in mammals and suggests that Rab27b may be a future drug target for allergic diseases, such as asthma.
Gene_expression (contributes) of Rab27b in mast cell associated with asthma, targeted disruption, hypersensitivity and disease
9) Confidence 0.67 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0.65 Pain Relevance 0
Our studies suggest that when Rab27b is absent, then Rab27a may partially compensate for its loss as a positive regulator of exocytosis, given that the secretory defect in the double KO cells is more severe than in the single Rab27b KO.
Neg (absent) Gene_expression (absent) of Rab27b associated with targeted disruption
10) Confidence 0.67 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0.52 Pain Relevance 0.03
In fact, the restricted pattern of expression observed for Rab27b compared with Rab27a further strengthens this possibility, suggesting that interfering with Rab27b function may lead to rather specific effects.
Gene_expression (expression) of Rab27b
11) Confidence 0.67 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0.63 Pain Relevance 0.03
Generation of Rab27b KO and double Rab27 KO mice
Gene_expression (Generation) of Rab27b associated with targeted disruption
12) Confidence 0.60 Published 2007 Journal Traffic (Copenhagen, Denmark) Section Body Doc Link PMC2063611 Disease Relevance 0.63 Pain Relevance 0.04
The lack of phenoytpe in the transgenic mice expressing dominant-negative mutant Rab27a and Rab27b could have been due to non-functional mutant proteins.
Gene_expression (expressing) of Rab27b associated with targeted disruption
13) Confidence 0.54 Published 2002 Journal BMC Cell Biol Section Body Doc Link PMC137576 Disease Relevance 0.59 Pain Relevance 0
Membranes were prehybridised and hybridised with Rab27a or Rab27b probe, corresponding to the entire Rab27 coding sequence, radiolabelled with 20 ?
Gene_expression (probe) of Rab27b
14) Confidence 0.47 Published 2002 Journal BMC Cell Biol Section Body Doc Link PMC137576 Disease Relevance 0.37 Pain Relevance 0
To quantify approximately the level of expression, the Rab27a or Rab27b product was compared to Hprt product (Fig. 3C and Table 3).
Gene_expression (product) of Rab27b
15) Confidence 0.47 Published 2002 Journal BMC Cell Biol Section Body Doc Link PMC137576 Disease Relevance 0.68 Pain Relevance 0
Lower levels of transgenic protein expression were observed for the other lines expressing dominant-negative mutant Rab27a and Rab27b (data not shown).
Gene_expression (expressing) of Rab27b associated with targeted disruption
16) Confidence 0.42 Published 2002 Journal BMC Cell Biol Section Body Doc Link PMC137576 Disease Relevance 0.50 Pain Relevance 0
We report the generation of several transgenic lines expressing dominant-negative mutants of Rab27a and Rab27b where no phenotype could be elicited.
Gene_expression (expressing) of Rab27b associated with targeted disruption
17) Confidence 0.42 Published 2002 Journal BMC Cell Biol Section Body Doc Link PMC137576 Disease Relevance 0.53 Pain Relevance 0
To investigate this hypothesis, we generated several lines of dominant-negative, constitutively-active and wild-type Rab27a (and Rab27b) transgenic mice whose expression was driven either by the pigment cell-specific tyrosinase promoter or the ubiquitous ?
Gene_expression (expression) of Rab27b in pigment cell associated with targeted disruption
18) Confidence 0.42 Published 2002 Journal BMC Cell Biol Section Abstract Doc Link PMC137576 Disease Relevance 1.00 Pain Relevance 0
High levels of mRNA and protein were observed in transgenic lines expressing wild-type or constitutively active Rab27a and Rab27b.
Gene_expression (expressing) of Rab27b associated with targeted disruption
19) Confidence 0.42 Published 2002 Journal BMC Cell Biol Section Abstract Doc Link PMC137576 Disease Relevance 0.98 Pain Relevance 0

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