INT17300

Context Info Confidence 0.11 First Reported 1991 Last Reported 1998 Negated 0 Speculated 0 Reported most in Abstract Documents 4 Total Number 5 Disease Relevance 0 Pain Relevance 3.28

cytosol (Acot1) mitochondrion (Acot1) peptidase activity (Prss12) lipid metabolic process (Acot1) cytoplasm (Acot1)

Anatomy Link Frequency substantia nigra 2 Prss12 (Rattus norvegicus) Acot1 (Rattus norvegicus)

Pain Link Frequency Relevance Heat gABA 22 100.00 Substantia nigra 2 99.92 Glutamate 10 99.40 agonist 1 99.04 tetrodotoxin 5 98.16 antagonist 9 96.24 Dopamine 12 95.80 Hippocampus 12 88.48 GABAergic 4 82.16 interneuron 2 50.40

Disease Link Frequency Relevance Heat Disease 1 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

The second set of experiments tested the effects of 6-hydroxydopamine (6-OHDA) lesions of the substantia nigra on NT-induced increases of potassium-evoked ACh release.

NT Positive_regulation (increases) of Localization (release) of ACh in substantia nigra associated with substantia nigra

1) Confidence 0.11 Published 1991 Journal J. Neurochem. Section Abstract Doc Link 1899109 Disease Relevance 0 Pain Relevance 0.32

NT enhanced potassium (25 mM)-evoked ACh release from striatal slices in a dose-dependent manner.

NT Positive_regulation (enhanced) of Localization (release) of ACh

2) Confidence 0.09 Published 1991 Journal J. Neurochem. Section Abstract Doc Link 1899109 Disease Relevance 0 Pain Relevance 0.31

Local infusion of tetrodotoxin (1 microM) decreased the basal release of ACh, GABA, Asp, Glu and prevented the 0.2 microM NT-induced increase in GABA and ACh release.

NT Positive_regulation (increase) of Localization (release) of ACh associated with tetrodotoxin, glutamate and gaba

3) Confidence 0.04 Published 1998 Journal Neurochem. Int. Section Abstract Doc Link 9840224 Disease Relevance 0 Pain Relevance 1.13

Local infusion of tetrodotoxin (1 microM) decreased the basal release of ACh, GABA, Asp, Glu and prevented the 0.2 microM NT-induced increase in GABA and ACh release.

NT Positive_regulation (induced) of Localization (release) of ACh associated with tetrodotoxin, glutamate and gaba

4) Confidence 0.04 Published 1998 Journal Neurochem. Int. Section Abstract Doc Link 9840224 Disease Relevance 0 Pain Relevance 1.13

However, the D2 receptor agonist quinpirole, 0.01 mM, significantly reduced the NT-induced release of ACh by 77%.

NT Positive_regulation (induced) of Localization (release) of ACh associated with agonist

5) Confidence 0.03 Published 1993 Journal Brain Res. Section Abstract Doc Link 8101133 Disease Relevance 0 Pain Relevance 0.40

General Comments

This test has worked.