INT176183

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Context Info
Confidence 0.77
First Reported 2004
Last Reported 2004
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 22
Disease Relevance 5.01
Pain Relevance 1.08

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

transport (Abcg5) ATPase activity (Abcg5)
Anatomy Link Frequency
liver 6
bile 1
intestine 1
Abcg5 (Mus musculus)
Pain Link Frequency Relevance Heat
Bile 946 100.00 Very High Very High Very High
agonist 66 99.96 Very High Very High Very High
Arthritis 22 43.96 Quite Low
anesthesia 44 5.00 Very Low Very Low Very Low
isoflurane 22 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Targeted Disruption 836 99.68 Very High Very High Very High
Disease 132 97.32 Very High Very High Very High
Disorder Of Lipid Metabolism 110 94.20 High High
Sprains And Strains 22 93.36 High High
Increased Venous Pressure Under Development 22 45.20 Quite Low
Arthritis 22 43.96 Quite Low
Arthralgia 22 43.52 Quite Low
Xanthomatosis 22 42.20 Quite Low
Lipidosis 22 34.72 Quite Low
Coronary Artery Disease 22 9.92 Low Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To investigate the effects of a deficiency of Abcg8/sterolin-2 on the genes that regulate sterol metabolism, quantitative RT-PCR was performed looking at the expression levels of Abcg5, Abcg8, Hmgr, Cyp7a1, Abca1, Mdr2, Lxr, Srebp-1c, and Srebp-2 mRNA in the livers of mice fed a regular chow diet (Figure 4a).
Gene_expression (expression) of Abcg5 in livers
1) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.26 Pain Relevance 0
However, by immunohistochemistry it appears that Abcg5/sterolin-1 is apically expressed in the Abcg8-deficient mice regardless of the anti-Abcg5/sterolin-1 antibody used.
Gene_expression (expressed) of Abcg5
2) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0 Pain Relevance 0.04
The exact function of these two proteins still remains unknown but recent studies have shown that overexpression of ABCG5/ABCG8 in a transgenic mouse model results in an increased biliary cholesterol concentration and a 50% reduction in the fractional absorption of dietary cholesterol [17].
Gene_expression (overexpression) of ABCG5 associated with targeted disruption and disorder of lipid metabolism
3) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.34 Pain Relevance 0.03
To further confirm the apical expression of Abcg5/sterolin-1 in the knockout mice, the UTSW antibody was used to stain liver sections of wild-type and knockout mice.
Gene_expression (expression) of Abcg5 in liver associated with targeted disruption
4) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.52 Pain Relevance 0.14
Co-transfection of COS-1 cells with both Abcg5 and Abcg8 cDNAs did not alter the pattern of immunofluorescence, although we are not able to confirm simultaneous expression of Abcg8 in cells expressing Abcg5/sterolin-1, as we have no suitable antibody for Abcg8/sterolin-2 (Figure 6d).
Gene_expression (expressing) of Abcg5
5) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.14 Pain Relevance 0
In acute transfection studies, the SC antibody recognized over-expressed mouse Abcg5/sterolin-1 in COS-1 cells, but not mouse Abcg8/sterolin-2 (Figure 6b,6c).
Gene_expression (expressed) of Abcg5
6) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.16 Pain Relevance 0
Again, there is apical expression of Abcg5/sterolin-1 in both wild-type and knockout livers (Figure 7a,7b).


Gene_expression (expression) of Abcg5 in livers associated with targeted disruption
7) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.53 Pain Relevance 0.19
To further confirm that Abcg5/sterolin-1 expression is preserved and may be apical, immunohistochemistry was performed in Amsterdam using the AMC antibody to stain liver sections from wild-type and knockout mice [30].
Gene_expression (expression) of Abcg5 in liver associated with targeted disruption
8) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.16 Pain Relevance 0.04
agonist, Abcg5/sterolin-1 and Abcg8/sterolin-2 appeared to be expressed at the apical surface.
Gene_expression (expressed) of Abcg5 associated with agonist
9) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0 Pain Relevance 0.05
Although not quantitative, Northern analyses showed that while the knockout mice showed no detectable mRNA for Abcg8, expression of Abcg5 appeared unaltered (Figure 1c).
Gene_expression (expression) of Abcg5 associated with targeted disruption
10) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.19 Pain Relevance 0
Again, both proteins are apically localized in liver sections from wild-type mice and this pattern is essentially unperturbed in liver from knockout mice, although the expression level of Abcg5/sterolin-1 seems to be less robust qualitatively (Figure 6l,6m).
Gene_expression (expression) of Abcg5 in liver associated with targeted disruption
11) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.49 Pain Relevance 0.11
Therefore, at this time, we can neither exclude the possibility that Abcg5/sterolin-1 expression is functional in our Abcg8/sterolin-2 deficient mice nor can we rule it out.
Gene_expression (expression) of Abcg5
12) Confidence 0.77 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0 Pain Relevance 0.07
Co-expression of both was required for apical trafficking in a polarized cell line, as well in vivo using Abcg5/Abcg8 double-knockout mice [18,19].
Gene_expression (using) of Abcg5 associated with targeted disruption
13) Confidence 0.67 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.46 Pain Relevance 0
For Dapi staining, 359 nm (30.38 ms exposure) was used and for Abcg5 and Bsep staining, 514 nm (for both: 1654.34 ms exposure) was used and the pictures were analyzed and overlaid using FW4000 software (Leica).


Gene_expression (staining) of Abcg5
14) Confidence 0.67 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0 Pain Relevance 0
As a control, the expression of Abcb11, an ABC transporter known to be responsible for the export of bile salts, was compared to that of Abcg5/sterolin-1 (Figure 6j,6k).
Gene_expression (expression) of Abcg5 in bile associated with bile
15) Confidence 0.67 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.38 Pain Relevance 0.08
Interestingly, by quantitative RT-PCR, the mRNA expression of Abcg5 in the knock-out mice was also reduced by more than 60% compared to the wild-type mice, although no changes were noted in the heterozygous mice.
Gene_expression (expression) of Abcg5 associated with targeted disruption
16) Confidence 0.60 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.24 Pain Relevance 0
In their studies, although diosgenin-fed mice showed a marked increase in biliary cholesterol output, mRNA levels of Abcg5 and Abcg8 were not altered.
Gene_expression (levels) of Abcg5
17) Confidence 0.60 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.09 Pain Relevance 0
Does the loss of Abcg8/sterolin-2 result in loss of Abcg5/sterolin-1 expression, as might be predicted from the genetic studies and more recently from the in vitro and in vivo expression studies?
Gene_expression (expression) of Abcg5
18) Confidence 0.60 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.39 Pain Relevance 0
The major findings in this study are: (1) disruption of only one of the two genes comprising the STSL locus is necessary and sufficient to cause sitosterolemia, (2) that the loss of Abcg8/sterolin-2 leads to the loss of biliary cholesterol secretion and (3) Abcg5/sterolin-1 appears to be still apically-expressed despite the absence of Abcg8/sterolin-2.
Gene_expression (expressed) of Abcg5
19) Confidence 0.60 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.05 Pain Relevance 0.13
Interestingly, Abcg5/sterolin-1 was expressed in both liver and intestine in Abcg8/sterolin-2 deficient mice and continued to show an apical expression.
Gene_expression (expressed) of Abcg5 in liver
20) Confidence 0.60 Published 2004 Journal BMC Med Section Abstract Doc Link PMC394351 Disease Relevance 0.26 Pain Relevance 0.04

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