INT176190

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Context Info
Confidence 0.78
First Reported 2004
Last Reported 2004
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 54
Disease Relevance 10.40
Pain Relevance 3.19

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

transport (Abcg8) ATPase activity (Abcg8)
Anatomy Link Frequency
liver 13
bile 5
capillary 3
intestine 1
Abcg8 (Mus musculus)
Pain Link Frequency Relevance Heat
Bile 2322 100.00 Very High Very High Very High
agonist 162 99.84 Very High Very High Very High
isoflurane 54 95.96 Very High Very High Very High
anesthesia 108 95.52 Very High Very High Very High
Arthritis 54 43.72 Quite Low
Disease Link Frequency Relevance Heat
Targeted Disruption 2052 99.76 Very High Very High Very High
Disease 324 97.68 Very High Very High Very High
Disorder Of Lipid Metabolism 270 94.44 High High
Sprains And Strains 54 93.36 High High
Increased Venous Pressure Under Development 54 44.96 Quite Low
Arthritis 54 43.72 Quite Low
Arthralgia 54 43.28 Quite Low
Xanthomatosis 54 41.96 Quite Low
Lipidosis 54 34.48 Quite Low
Dislocations 54 32.08 Quite Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To investigate the effects of a deficiency of Abcg8/sterolin-2 on the genes that regulate sterol metabolism, quantitative RT-PCR was performed looking at the expression levels of Abcg5, Abcg8, Hmgr, Cyp7a1, Abca1, Mdr2, Lxr, Srebp-1c, and Srebp-2 mRNA in the livers of mice fed a regular chow diet (Figure 4a).
Gene_expression (expression) of Abcg8 in livers
1) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.26 Pain Relevance 0
Therefore, at this time, we can neither exclude the possibility that Abcg5/sterolin-1 expression is functional in our Abcg8/sterolin-2 deficient mice nor can we rule it out.
Gene_expression (expression) of sterolin
2) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0 Pain Relevance 0.07
To further confirm that Abcg5/sterolin-1 expression is preserved and may be apical, immunohistochemistry was performed in Amsterdam using the AMC antibody to stain liver sections from wild-type and knockout mice [30].
Gene_expression (expression) of sterolin in liver associated with targeted disruption
3) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.16 Pain Relevance 0.04
agonist, Abcg5/sterolin-1 and Abcg8/sterolin-2 appeared to be expressed at the apical surface.
Gene_expression (expressed) of sterolin associated with agonist
4) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0 Pain Relevance 0.05
agonist, Abcg5/sterolin-1 and Abcg8/sterolin-2 appeared to be expressed at the apical surface.
Gene_expression (expressed) of sterolin associated with agonist
5) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0 Pain Relevance 0.05
In acute transfection studies, the SC antibody recognized over-expressed mouse Abcg5/sterolin-1 in COS-1 cells, but not mouse Abcg8/sterolin-2 (Figure 6b,6c).
Gene_expression (expressed) of sterolin
6) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.16 Pain Relevance 0
The exact function of these two proteins still remains unknown but recent studies have shown that overexpression of ABCG5/ABCG8 in a transgenic mouse model results in an increased biliary cholesterol concentration and a 50% reduction in the fractional absorption of dietary cholesterol [17].
Gene_expression (overexpression) of ABCG8 associated with targeted disruption and disorder of lipid metabolism
7) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.34 Pain Relevance 0.03
Again, both proteins are apically localized in liver sections from wild-type mice and this pattern is essentially unperturbed in liver from knockout mice, although the expression level of Abcg5/sterolin-1 seems to be less robust qualitatively (Figure 6l,6m).
Gene_expression (expression) of sterolin in liver associated with targeted disruption
8) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.49 Pain Relevance 0.11
It has been shown that Abcg8/sterolin-2 expression is required for correct apical trafficking of Abcg5/sterolin-1 to the apical surface in a polarized cell line and more recently in vivo by over-expression experiments [18,19].
Gene_expression (expression) of Abcg8
9) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0 Pain Relevance 0
Again, there is apical expression of Abcg5/sterolin-1 in both wild-type and knockout livers (Figure 7a,7b).


Gene_expression (expression) of sterolin in livers associated with targeted disruption
10) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.53 Pain Relevance 0.19
Co-transfection of COS-1 cells with both Abcg5 and Abcg8 cDNAs did not alter the pattern of immunofluorescence, although we are not able to confirm simultaneous expression of Abcg8 in cells expressing Abcg5/sterolin-1, as we have no suitable antibody for Abcg8/sterolin-2 (Figure 6d).
Gene_expression (expressing) of sterolin
11) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.14 Pain Relevance 0
Co-transfection of COS-1 cells with both Abcg5 and Abcg8 cDNAs did not alter the pattern of immunofluorescence, although we are not able to confirm simultaneous expression of Abcg8 in cells expressing Abcg5/sterolin-1, as we have no suitable antibody for Abcg8/sterolin-2 (Figure 6d).
Gene_expression (expression) of Abcg8
12) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.14 Pain Relevance 0
It has been shown that Abcg8/sterolin-2 expression is required for correct apical trafficking of Abcg5/sterolin-1 to the apical surface in a polarized cell line and more recently in vivo by over-expression experiments [18,19].
Gene_expression (expression) of sterolin
13) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0 Pain Relevance 0
To further confirm the apical expression of Abcg5/sterolin-1 in the knockout mice, the UTSW antibody was used to stain liver sections of wild-type and knockout mice.
Gene_expression (expression) of sterolin in liver associated with targeted disruption
14) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.52 Pain Relevance 0.15
However, by immunohistochemistry it appears that Abcg5/sterolin-1 is apically expressed in the Abcg8-deficient mice regardless of the anti-Abcg5/sterolin-1 antibody used.
Gene_expression (expressed) of sterolin
15) Confidence 0.78 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0 Pain Relevance 0.04
Thus knocking out Abcg8/sterolin-2 alone is equivalent to a functional loss of both sterolins.
Gene_expression (knocking) of sterolin
16) Confidence 0.68 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.05 Pain Relevance 0
As expected, Abcg8 mRNA expression levels were undetectable in the Abcg8-/- mice and were reduced by ~50% in the heterozygous mice, relative to wild-type mice.
Gene_expression (expression) of Abcg8
17) Confidence 0.68 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.27 Pain Relevance 0
Furthermore, complete loss of Abcg8/sterolin-2 results in an inability to secrete cholesterol into bile, although secretion of sitosterol seemed to be preserved.
Gene_expression (/) of sterolin in bile associated with bile
18) Confidence 0.68 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.22 Pain Relevance 0.28
Biliary secretion of bile salts, sterol and phospholipids in Abcg8/sterolin-2 deficient mice
Gene_expression (/) of sterolin in bile associated with bile
19) Confidence 0.68 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.52 Pain Relevance 0.21
Liver gene expression and enzyme activity change in Abcg8/sterolin-2 deficient mice
Gene_expression (/) of Abcg8 in Liver
20) Confidence 0.68 Published 2004 Journal BMC Med Section Body Doc Link PMC394351 Disease Relevance 0.21 Pain Relevance 0

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