INT177551

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Context Info
Confidence 0.37
First Reported 2004
Last Reported 2010
Negated 0
Speculated 1
Reported most in Body
Documents 31
Total Number 32
Disease Relevance 11.16
Pain Relevance 2.65

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
Blood vessel 3
neurons 3
retina 1
muscles 1
endometrium 1
Tg(CAG-EGFP)D4Nagy (Mus musculus)
Pain Link Frequency Relevance Heat
Dorsal horn 33 99.68 Very High Very High Very High
Spinal cord 137 99.64 Very High Very High Very High
imagery 115 99.12 Very High Very High Very High
ischemia 12 97.12 Very High Very High Very High
nMDA receptor 81 91.96 High High
substantia gelatinosa 4 89.92 High High
Raphe 2 89.20 High High
dorsal root ganglion 55 89.04 High High
Locus ceruleus 20 88.16 High High
Calcitonin gene-related peptide 36 85.88 High High
Disease Link Frequency Relevance Heat
Cancer 482 100.00 Very High Very High Very High
Brain Hemorrhage 24 99.44 Very High Very High Very High
Cold Sores 139 99.36 Very High Very High Very High
Targeted Disruption 317 97.60 Very High Very High Very High
Skin Cancer 31 97.16 Very High Very High Very High
Cv Unclassified Under Development 16 97.12 Very High Very High Very High
Glioma 30 96.96 Very High Very High Very High
Pressure And Volume Under Development 54 96.40 Very High Very High Very High
Cytomegalovirus Infection 41 93.96 High High
Toxicity 15 91.08 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Furthermore, we defined experimental systems to visualize and quantify down-regulation of a constitutively expressed eGFP reporter gene, and the localization of siRNAs in subcutaneous B16-F10 melanoma tumors using fluorescence imaging.
Negative_regulation (regulation) of eGFP in B16-F10 associated with cancer, skin cancer and imagery
1) Confidence 0.37 Published 2008 Journal Journal of RNAi and Gene Silencing : An International Journal of RNA and Gene Targeting Research Section Body Doc Link PMC2737239 Disease Relevance 0.37 Pain Relevance 0.09
The decrease of eGFP fluorescence observed upon eGFP22 Alexa labeled siRNA electrotransfer was not observed in the different control groups (Figure 4).
Negative_regulation (decrease) of eGFP
2) Confidence 0.36 Published 2008 Journal Journal of RNAi and Gene Silencing : An International Journal of RNA and Gene Targeting Research Section Body Doc Link PMC2737239 Disease Relevance 0.44 Pain Relevance 0.04
Thus a decrease in the fluorescence intensity of eGFP indicates that electropermeabilization resulted in free cellular loading of labeled siRNA in active form.


Negative_regulation (decrease) of eGFP
3) Confidence 0.36 Published 2008 Journal Journal of RNAi and Gene Silencing : An International Journal of RNA and Gene Targeting Research Section Body Doc Link PMC2737239 Disease Relevance 0.25 Pain Relevance 0
The levels of EGFP mRNA in the lens were relatively equal between eyes receiving the AC-GFP or TFA-GFP nanoparticle, however in the retina and PECS a difference was detected between the two nanoparticles.
Negative_regulation (receiving) of AC-GFP in retina
4) Confidence 0.32 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762345 Disease Relevance 0.30 Pain Relevance 0.03
After electrotransfer of the Alexa labeled siRNA (+EP), GFP fluorescence in the tumors was reduced as compared with the control tumors.
Negative_regulation (reduced) of GFP associated with cancer
5) Confidence 0.32 Published 2008 Journal Journal of RNAi and Gene Silencing : An International Journal of RNA and Gene Targeting Research Section Body Doc Link PMC2737239 Disease Relevance 0.99 Pain Relevance 0
Fluorescent microscopy at 48hrs post-transduction revealed that transduction with HSV-U6shLacZ had no effect on GFP expression levels, whereas transduction with HSV-U6shGFP resulted in a dramatic reduction in the levels of GFP compared to control groups (magnification 10×).


Negative_regulation (reduction) of GFP associated with cold sores
6) Confidence 0.21 Published 2008 Journal Nucleic Acids Research Section Body Doc Link PMC2504301 Disease Relevance 1.62 Pain Relevance 0.19
Each treatment yielded deposition of EGFP fluorescence in some organs including the lung, heart, kidney and pancreas.
Negative_regulation (deposition) of EGFP in lung
7) Confidence 0.15 Published 2004 Journal BMC Nephrol Section Body Doc Link PMC419703 Disease Relevance 0.06 Pain Relevance 0
GFP was notably reduced in the dorsal horn ipsilateral to the severed dorsal roots in contrast to abundant GFP labeling on the contralateral, unoperated side (Fig. 4A and 4C).
Negative_regulation (reduced) of GFP in dorsal roots associated with dorsal horn
8) Confidence 0.14 Published 2010 Journal Mol Pain Section Body Doc Link PMC2900238 Disease Relevance 0 Pain Relevance 0.50
Monocyte recruitment was little affected by loss of the second CX3CR1 allele (Ccr2RFP/+Cx3cr1GFP/GFP), but was markedly reduced by loss of the second CCR2 allele (Ccr2RFP/RFPCx3cr1GFP/+) (Figure 3B).
Negative_regulation (loss) of GFP in Monocyte
9) Confidence 0.14 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.32 Pain Relevance 0.03
We found that the NMDA to AMPA/KA ratio was significantly reduced in GFP-positive cells (GFP-positive 0.068?
Negative_regulation (reduced) of GFP
10) Confidence 0.13 Published 2007 Journal Frontiers in Integrative Neuroscience Section Body Doc Link PMC2526007 Disease Relevance 0.07 Pain Relevance 0.06
The reason for absence of GFP-ir in the SG is unclear.
Negative_regulation (absence) of GFP
11) Confidence 0.12 Published 2010 Journal Mol Pain Section Body Doc Link PMC2900238 Disease Relevance 0.05 Pain Relevance 0.44
Under these conditions, ifenprodil decreased the amplitude of NMDA EPSC to 64.4 ± 8.3% (n = 8, p < 0.05, paired t-test) for EGFP+ neurons and to 63.5 ± 6.0% (n = 8, p < 0.05, paired t-test) for EGFP- neurons (Figure 6A and 6B).
Negative_regulation (decreased) of EGFP in neurons
12) Confidence 0.12 Published 2010 Journal Mol Pain Section Body Doc Link PMC2879240 Disease Relevance 0 Pain Relevance 0.30
Figure 9B is the vlPAG area from a representative FVB-GFP mouse given the neurotoxin and shows the loss of the GFP neurons along with NeuN neurons.
Negative_regulation (loss) of GFP in neurons
13) Confidence 0.08 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2709920 Disease Relevance 0 Pain Relevance 0.11
The lack of EGFP was puzzling.
Negative_regulation (lack) of EGFP
14) Confidence 0.08 Published 2006 Journal PLoS Genet Section Body Doc Link PMC1358942 Disease Relevance 0.21 Pain Relevance 0
Extent of lesion was marked by the boundary of the neuronal loss as seen by the NeuN staining and loss of the GFP neurons.
Negative_regulation (loss) of GFP in neurons
15) Confidence 0.07 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2709920 Disease Relevance 0.20 Pain Relevance 0.09
In all cases, knockdown of the marker transgene GFP in neonates was reported.
Negative_regulation (knockdown) of GFP
16) Confidence 0.07 Published 2006 Journal PLoS Genet Section Body Doc Link PMC1358942 Disease Relevance 0.33 Pain Relevance 0
In contrast, none of the NMJs in MG muscles from EGFP mice transplanted into wild-type mice exhibited EGFP fluorescence (Figure 5B).
Negative_regulation (exhibited) of EGFP in muscles
17) Confidence 0.07 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2842435 Disease Relevance 0.21 Pain Relevance 0
To confirm the presence of donor cells in the tissues detected by ex vivo fluorescent imaging, sections of the tissues were examined for presence of EGFP+ cells 24 h after transplantation (Fig. 3D).
Spec (examined) Negative_regulation (presence) of EGFP associated with imagery
18) Confidence 0.07 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2885427 Disease Relevance 0 Pain Relevance 0.32
Interestingly, the absence of CX3CR1 in the Ccr2RFP/+Cx3cr1GFP/GFP mice had little or no effect on the RFP/GFP profile or the number of recruited cells.
Negative_regulation (absence) of GFP
19) Confidence 0.06 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.96 Pain Relevance 0.21
The subsequent growth of cerebral infarcts was easily followed as the loss of GFP fluorescence as astrocytes lysed.
Negative_regulation (loss) of GFP in astrocytes associated with brain hemorrhage
20) Confidence 0.06 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC3008710 Disease Relevance 1.04 Pain Relevance 0.09

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