INT178300

Context Info Confidence 0.75 First Reported 2004 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 2 Total Number 11 Disease Relevance 4.85 Pain Relevance 0.21

pigmentation (Hps3) cytoplasm (Hps3)

Anatomy Link Frequency blood 1 Hps3 (Mus musculus)

Pain Link Frequency Relevance Heat Hippocampus 12 84.04 Pain 11 65.24 Inflammation 20 5.00 Arthritis 10 5.00 Central nervous system 3 5.00 long-term potentiation 1 5.00 Spinal cord 1 5.00 Nerve growth factor 1 5.00 tolerance 1 5.00 medulla 1 5.00

Disease Link Frequency Relevance Heat Sprains And Strains 250 99.48 Coagulation Disorder 180 98.80 Abscess 630 98.76 Bacteremia 100 96.92 Infection 150 93.60 Staphylococcus Infection 150 89.44 Death 40 89.08 Shock 10 76.36 Akathisia 10 75.60 Volume Depletion And Dehydration 10 71.60

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

When probed for expression of coagulases with specific antibodies, we observed Coa secretion by wild-type S. aureus as well as the ?

Gene_expression (expression) of coa

1) Confidence 0.75 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.61 Pain Relevance 0

Coagulase production is used as a diagnostic test, differentiating S. aureus isolates from commensal staphylococci, for example S. epidermidis [18].

Gene_expression (production) of Coa

2) Confidence 0.75 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.53 Pain Relevance 0

For the coagulase-fibrinogen interaction, coagulase was injected at 100 pM, 500 pM, 1 nM, 5 nM, 10 nM, 25 nM, 50 nM, 100 nM, and 300 nM for 60 seconds of association followed by 60 seconds of dissociation and the chip was regenerated as described above.

Gene_expression (injected) of coa

3) Confidence 0.65 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0 Pain Relevance 0

Measurements of coagulase activity

Gene_expression (activity) of coa

4) Confidence 0.65 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0 Pain Relevance 0

In contrast, deletion of both coagulase genes caused a dramatic defect; ?

Gene_expression (deletion) of coa

5) Confidence 0.65 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.76 Pain Relevance 0

Introduction of pvWbp-coa, a plasmid providing for the expression of both coagulase genes, into ?

Gene_expression (expression) of coa

6) Confidence 0.65 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.59 Pain Relevance 0

For the coagulase-fibrinogen interaction, coagulase was injected at 100 pM, 500 pM, 1 nM, 5 nM, 10 nM, 25 nM, 50 nM, 100 nM, and 300 nM for 60 seconds of association followed by 60 seconds of dissociation and the chip was regenerated as described above.

Gene_expression (injected) of coa

7) Confidence 0.65 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0 Pain Relevance 0

Antibodies against coagulases affect staphylococcal clotting of blood

Gene_expression (Antibodies) of coa in blood associated with coagulation disorder

8) Confidence 0.65 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.67 Pain Relevance 0

DNA sequences 1 kb upstream and downstream of coa and vWbp were PCR amplified using the primers attB1_Coa, Coa1_BamHI, Coa2_BamHI, attbB2_Coa and attB1_vWF, vWF1_BamHI, vWF2_BamHI, attbB2_vWF (Table S1).

Gene_expression (using) of Coa

9) Confidence 0.65 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.84 Pain Relevance 0.07

DNA sequences 1 kb upstream and downstream of coa and vWbp were PCR amplified using the primers attB1_Coa, Coa1_BamHI, Coa2_BamHI, attbB2_Coa and attB1_vWF, vWF1_BamHI, vWF2_BamHI, attbB2_vWF (Table S1).

Gene_expression (using) of Coa

10) Confidence 0.29 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.84 Pain Relevance 0.07

2610008O03Rik protein (Q9D0K2) has two conserved domains, coenzyme A (CoA) transferases (pfam01144) (Fig. 3), and belongs to an evolutionary conserved family of enzymes catalyzing the reversible transfer of CoA from one carboxylic acid to another.

Gene_expression (transfer) of CoA

11) Confidence 0.01 Published 2004 Journal Proteome Sci Section Body Doc Link PMC446209 Disease Relevance 0 Pain Relevance 0.08

General Comments

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