INT180534
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
We also analysed the patients who were admitted to hospice wards during the peak period of the SARS epidemic (i.e. | |||||||||||||||
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Thiel and colleagues performed the first detailed study on mechanisms and enzymes involved in SARS-CoV genome expression (Figure 2) [29]. | |||||||||||||||
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Upon flowing over the adsorbed enzyme a deoxygenated and buffered DX solution containing sodium dithionite as a reducing agent, the SERRS spectrum shown in Fig. 2, spectrum f is observed. | |||||||||||||||
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In fact, in all the RR or SERRS spectra of P420 species reported so far for various CYPs, the ? | |||||||||||||||
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Recently, heme proteins such as cytochromes c [5, 1319] and CYP101 [20] have been investigated using SERRS on roughened Ag electrodes coated with self-assembled monolayers (SAMs) of several different alkanethiols and their derivatives. | |||||||||||||||
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Fig. 6Comparison between SERRS spectra of CYP2D6 on MUA-coated Ag electrodes a chemically reduced with dithionite (open circuit) and b at ? | |||||||||||||||
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Once the buffer had completely replaced the DX solution in the cell, 5cHS marker bands disappeared from SERRS spectra of CYP2D6, which recover the shape characteristic of the 6cLS heme in the resting state (Fig. 2, spectrum c). | |||||||||||||||
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An additional but less pronounced feature observed in the SERRS spectrum of the CYP2D6imidazole complex is the intensity decrease in the region around 1,630 cm? | |||||||||||||||
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In general, SERRS spectroscopy on coated Ag surfaces was proven to be successful in monitoring the active-site structure of immobilized CYP2D6, enabling the detection of reversible substrate and ligand binding using only very low amounts of protein. | |||||||||||||||
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Such variation might also cause a shift in the stretching frequencies of the vinyls [49, 50], although in SERRS spectra of CYP2D6 no significant shifts are detected. | |||||||||||||||
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Similarly to what was observed for DX binding, SERRS spectra obtained by flowing buffer through the cell in place of the imidazole solution (Fig. 2, spectrum e) have the ? | |||||||||||||||
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4) regions of SERRS spectra of CYP2D6 on MUA-coated Ag electrodes at ? | |||||||||||||||
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The SERRS spectra of CYP2D6 collected upon flowing an imidazole solution over the enzyme-coated electrode are shown in Fig. 2, spectra d, displaying marker bands characteristic of a 6cLS state (Table 1), as expected from a CYP heme having an imidazole as the sixth axial ligand. | |||||||||||||||
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Notably, this partial inactivation is not due to the immobilization as such, since every attempt to obtain reduced free CYP2D6 in solution by dithionite addition led to samples containing inactive enzyme besides the active one, yielding RR spectra which closely resemble the SERRS spectrum of the adsorbed enzyme (Fig. 4, spectra a). | |||||||||||||||
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We analyzed the expression pattern of over ~8,700 genes from the PBMCs of 10 SARS patients, and compared the expression patterns with healthy control samples. | |||||||||||||||
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We analyzed the expression pattern of over ~8,700 genes from the PBMCs of 10 SARS patients, and compared the expression patterns with healthy control samples. | |||||||||||||||
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Using this technology, complemented with powerful analytical methods, we compared the gene expression profiles of PBMC from a series of SARS patients with those of healthy donors. | |||||||||||||||
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Next to these epithelial cells, the SARS-CoV has also been found in macrophages and many other cells as it has been detected in not only in the respiratory tract and stool specimen but also in the blood, liver, kidney and urine [6]. | |||||||||||||||
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Eight recombinant human single-chain variable region fragments (scFvs) against the S1 domain of spike (S) protein of the SARS-CoV from two nonimmune human antibody libraries were screened and one scFv 80R efficiently neutralized SARS-CoV and inhibited syncytia formation between cells expressing the S protein and those expressing the SARS-CoV receptor angiotensin-converting enzyme 2 (ACE2) [117]. | |||||||||||||||
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As it is crucial for future strategies that SARS is detected in its earliest stages and that therapeutic options are optimized, insights into the molecular mechanism of SARS have to be used to develop new therapeutic strategies and vaccines. | |||||||||||||||
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