INT180534

Context Info Confidence 0.59 First Reported 2005 Last Reported 2010 Negated 2 Speculated 1 Reported most in Body Documents 40 Total Number 42 Disease Relevance 35.47 Pain Relevance 0.81

cytosol (SARS) mitochondrion (SARS) RNA binding (SARS) translation (SARS) ligase activity (SARS) cytoplasm (SARS)

Anatomy Link Frequency bands 2 plasma 1 bloods 1 spike 1 immune system 1 SARS (Homo sapiens)

Pain Link Frequency Relevance Heat Dextromethorphan 360 99.80 Potency 19 94.32 cytokine 80 67.88 headache 37 65.92 Inflammatory response 14 55.84 chemokine 9 36.52 fluoxetine 12 21.52 Inflammation 87 7.80 fibrosis 33 5.00 cINOD 20 5.00

Disease Link Frequency Relevance Heat Severe Acute Respiratory Syndrome 3461 100.00 Coronavirus Infection 630 100.00 Infection 629 100.00 Disease 553 100.00 Bacterial Infection 129 100.00 Acquired Immune Deficiency Syndrome Or Hiv Infection 103 99.04 Cancer 80 96.44 Congenital Anomalies 20 95.20 Adult Respiratory Distress Syndrome 34 94.52 Lower Respiratory Tract Infection 13 94.36

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We also analysed the patients who were admitted to hospice wards during the peak period of the SARS epidemic (i.e.

Gene_expression (epidemic) of SARS associated with severe acute respiratory syndrome

1) Confidence 0.59 Published 2006 Journal BMC Health Serv Res Section Body Doc Link PMC1559606 Disease Relevance 0.19 Pain Relevance 0

Thiel and colleagues performed the first detailed study on mechanisms and enzymes involved in SARS-CoV genome expression (Figure 2) [29].

Gene_expression (expression) of SARS associated with severe acute respiratory syndrome

2) Confidence 0.40 Published 2005 Journal Respir Res Section Body Doc Link PMC548145 Disease Relevance 0.71 Pain Relevance 0

Upon flowing over the adsorbed enzyme a deoxygenated and buffered DX solution containing sodium dithionite as a reducing agent, the SERRS spectrum shown in Fig. 2, spectrum f is observed.

Gene_expression (spectrum) of SERRS associated with dextromethorphan

3) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0.05

In fact, in all the RR or SERRS spectra of P420 species reported so far for various CYPs, the ?

Gene_expression (spectra) of SERRS

4) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0

Recently, heme proteins such as cytochromes c [5, 13–19] and CYP101 [20] have been investigated using SERRS on roughened Ag electrodes coated with self-assembled monolayers (SAMs) of several different alkanethiols and their derivatives.

Gene_expression (using) of SERRS

5) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0

Fig. 6Comparison between SERRS spectra of CYP2D6 on MUA-coated Ag electrodes a chemically reduced with dithionite (open circuit) and b at ?

Gene_expression (spectra) of SERRS

6) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0.04

Once the buffer had completely replaced the DX solution in the cell, 5cHS marker bands disappeared from SERRS spectra of CYP2D6, which recover the shape characteristic of the 6cLS heme in the resting state (Fig. 2, spectrum c).

Gene_expression (spectra) of SERRS in bands associated with dextromethorphan

7) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0.15

An additional but less pronounced feature observed in the SERRS spectrum of the CYP2D6–imidazole complex is the intensity decrease in the region around 1,630 cm?

Gene_expression (spectrum) of SERRS

8) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0

In general, SERRS spectroscopy on coated Ag surfaces was proven to be successful in monitoring the active-site structure of immobilized CYP2D6, enabling the detection of reversible substrate and ligand binding using only very low amounts of protein.

Gene_expression (spectroscopy) of SERRS

9) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0.03

Such variation might also cause a shift in the stretching frequencies of the vinyls [49, 50], although in SERRS spectra of CYP2D6 no significant shifts are detected.

Neg (no) Gene_expression (spectra) of SERRS

10) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0

Similarly to what was observed for DX binding, SERRS spectra obtained by flowing buffer through the cell in place of the imidazole solution (Fig. 2, spectrum e) have the ?

Gene_expression (spectra) of SERRS associated with dextromethorphan

11) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0.05

4) regions of SERRS spectra of CYP2D6 on MUA-coated Ag electrodes at ?

Gene_expression (spectra) of SERRS

12) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0.05

The SERRS spectra of CYP2D6 collected upon flowing an imidazole solution over the enzyme-coated electrode are shown in Fig. 2, spectra d, displaying marker bands characteristic of a 6cLS state (Table 1), as expected from a CYP heme having an imidazole as the sixth axial ligand.

Gene_expression (spectra) of SERRS in bands

13) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0.03

Notably, this partial inactivation is not due to the immobilization as such, since every attempt to obtain reduced free CYP2D6 in solution by dithionite addition led to samples containing inactive enzyme besides the active one, yielding RR spectra which closely resemble the SERRS spectrum of the adsorbed enzyme (Fig. 4, spectra a).

Gene_expression (spectrum) of SERRS

14) Confidence 0.40 Published 2007 Journal J Biol Inorg Chem Section Body Doc Link PMC2099460 Disease Relevance 0 Pain Relevance 0.05

We analyzed the expression pattern of over ~8,700 genes from the PBMCs of 10 SARS patients, and compared the expression patterns with healthy control samples.

Gene_expression (expression) of SARS associated with severe acute respiratory syndrome

15) Confidence 0.36 Published 2005 Journal BMC Immunol Section Body Doc Link PMC546205 Disease Relevance 0.60 Pain Relevance 0

We analyzed the expression pattern of over ~8,700 genes from the PBMCs of 10 SARS patients, and compared the expression patterns with healthy control samples.

Spec (analyzed) Gene_expression (expression) of SARS associated with severe acute respiratory syndrome

16) Confidence 0.36 Published 2005 Journal BMC Immunol Section Body Doc Link PMC546205 Disease Relevance 0.62 Pain Relevance 0

Using this technology, complemented with powerful analytical methods, we compared the gene expression profiles of PBMC from a series of SARS patients with those of healthy donors.

Gene_expression (expression) of SARS associated with severe acute respiratory syndrome

17) Confidence 0.36 Published 2005 Journal BMC Immunol Section Body Doc Link PMC546205 Disease Relevance 0.95 Pain Relevance 0.06

Next to these epithelial cells, the SARS-CoV has also been found in macrophages and many other cells as it has been detected in not only in the respiratory tract and stool specimen but also in the blood, liver, kidney and urine [6].

Gene_expression (found) of SARS in epithelial cells associated with severe acute respiratory syndrome

18) Confidence 0.36 Published 2005 Journal Respir Res Section Body Doc Link PMC548145 Disease Relevance 1.26 Pain Relevance 0

Eight recombinant human single-chain variable region fragments (scFvs) against the S1 domain of spike (S) protein of the SARS-CoV from two nonimmune human antibody libraries were screened and one scFv 80R efficiently neutralized SARS-CoV and inhibited syncytia formation between cells expressing the S protein and those expressing the SARS-CoV receptor angiotensin-converting enzyme 2 (ACE2) [117].

Gene_expression (expressing) of SARS in spike associated with severe acute respiratory syndrome

19) Confidence 0.36 Published 2005 Journal Respir Res Section Body Doc Link PMC548145 Disease Relevance 1.22 Pain Relevance 0

As it is crucial for future strategies that SARS is detected in its earliest stages and that therapeutic options are optimized, insights into the molecular mechanism of SARS have to be used to develop new therapeutic strategies and vaccines.

Gene_expression (detected) of SARS associated with severe acute respiratory syndrome

20) Confidence 0.36 Published 2005 Journal Respir Res Section Body Doc Link PMC548145 Disease Relevance 1.58 Pain Relevance 0

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