INT182266

Context Info Confidence 0.35 First Reported 2005 Last Reported 2005 Negated 1 Speculated 0 Reported most in Body Documents 1 Total Number 5 Disease Relevance 2.03 Pain Relevance 0.63

extracellular space (EDN1, MMP13) extracellular region (EDN1, MMP13) Golgi apparatus (MMP13) cell-cell signaling (EDN1) cytoplasm (EDN1) peptidase activity (MMP13)

Anatomy Link Frequency chondrocytes 2 EDN1 (Homo sapiens) MMP13 (Homo sapiens)

Pain Link Frequency Relevance Heat Osteoarthritis 175 99.60 cytokine 15 90.40 rheumatoid arthritis 15 66.32 metalloproteinase 5 5.00 Inflammation 5 5.00 Pain 5 5.00

Disease Link Frequency Relevance Heat Osteoarthritis 180 99.60 Stress 15 94.76 Death 20 93.08 Apoptosis 70 92.48 Rheumatic Diseases 5 79.36 Rheumatoid Arthritis 15 66.32 Osteoporosis 5 12.04 Pain 5 5.00 Disease 5 5.00 INFLAMMATION 5 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

We previously demonstrated [2] that MMP-13 expression was induced similarly by ET-1 and IL-1?

ET-1 Positive_regulation (induced) of Gene_expression (expression) of MMP-13

1) Confidence 0.35 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1065327 Disease Relevance 0.44 Pain Relevance 0.22

Results show that ET-1 greatly increased MMP-1 and MMP-13 production, iNOS expression and NO release.

ET-1 Neg (NO) Positive_regulation (increased) of Gene_expression (production) of MMP-13

2) Confidence 0.27 Published 2005 Journal Arthritis Res Ther Section Abstract Doc Link PMC1065327 Disease Relevance 0.31 Pain Relevance 0.07

The present study shows that ET-1 causes an overproduction of NO, MMP-1 and MMP-13 in human OA chondrocytes.

ET-1 Positive_regulation (causes) of Gene_expression (overproduction) of MMP-13 in chondrocytes associated with osteoarthritis

3) Confidence 0.27 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1065327 Disease Relevance 0.95 Pain Relevance 0.14

The activation of these kinases is probably necessary for the induction by ET-1 of MMP-1 production and MMP-13 production.

ET-1 Positive_regulation (induction) of Gene_expression (production) of MMP-13

4) Confidence 0.25 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1065327 Disease Relevance 0.14 Pain Relevance 0.07

The inhibition of p38 kinase is associated with a suppression of the ET-1-induced stimulation of both enzymes, whereas the inhibitions of adenyl cyclase-dependent PKA kinase is associated with a partial suppression of the ET-1-induced stimulation of MMP-13 production only.

ET-1-induced Positive_regulation (stimulation) of Gene_expression (production) of MMP-13

5) Confidence 0.23 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1065327 Disease Relevance 0.20 Pain Relevance 0.13

General Comments

This test has worked.