INT182413

Context Info Confidence 0.46 First Reported 2005 Last Reported 2010 Negated 1 Speculated 2 Reported most in Body Documents 17 Total Number 21 Disease Relevance 2.44 Pain Relevance 0.22

nucleoplasm (OGG1) mitochondrion (OGG1) lyase activity (OGG1) hydrolase activity, acting on glycosyl bonds (OGG1) nucleolus (OGG1) nucleus (OGG1)

OGG1 (Homo sapiens) OGG1 - S326C (2)

Pain Link Frequency Relevance Heat imagery 17 75.84 Glutamate 16 70.88 Pain 17 8.44 ischemia 9 5.00 Inflammation 9 5.00 alcohol 4 5.00 cINOD 3 5.00 Inflammatory response 3 5.00 Crohn's disease 3 5.00 cerebral cortex 1 5.00

Disease Link Frequency Relevance Heat Genomic Instability 3 99.92 Myeloid Leukemia 7 99.80 Nasopharynx Cancer 103 97.76 Cancer 210 92.44 Cockayne Syndrome 32 86.56 Stress 78 83.28 Angiomyolipoma 12 80.36 Inflammatory Bowel Disease 21 79.60 Nicotine Addiction 2 77.68 Renal Disease 1 61.44

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Interestingly, the anti-phosphoserine recognized a phosphorylated form of the enzyme in both EGFP-hOGG1 and EGFP-hOGG1-Glu326 chromatin fractions.

EGFP-hOGG1 Binding (recognized) of

1) Confidence 0.46 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.06

We have previously shown that hOGG1 is associated with the soluble chromatin and the nuclear matrix during interphase and with condensed chromatin during mitosis (47).

hOGG1 Binding (associated) of

2) Confidence 0.40 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

As EGFP-hOGG1 has been shown to be associated with the soluble chromatin and the nuclear matrix during interphase (47), we wanted to investigate whether these associations were maintained for the EGFP-hOGG1-Cys326 mutant protein.

EGFP-hOGG1 Binding (associated) of

3) Confidence 0.40 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

By sequentially extracting the cells to obtain cytoplasm, soluble chromatin and the nuclear matrix, we were able to show that EGFP-hOGG1-Glu326 was associated with both the soluble chromatin and the nuclear matrix fractions (Figure 7D, lanes 2 and 4).

EGFP-hOGG1 Binding (associated) of

4) Confidence 0.34 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.06

Within the next 2 h, cells enter the M-phase, the nuclear envelope disassembles and EGFP-hOGG1 associates with chromatin (Figure 4).

EGFP-hOGG1 Binding (associates) of

5) Confidence 0.34 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

The pEYFP-hOGG1 plasmid was constructed by ligating the hOGG1 cDNA fragment from pEGFP-hOGG1 into the pEYFP-N1 vector.

hOGG1 Binding (ligating) of

6) Confidence 0.31 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

As neither a functional nor a physical direct interaction was detected between these two proteins, the authors hypothesize a protein complex containing among others CSB and hOGG1.

hOGG1 Neg (neither) Binding (interaction) of

7) Confidence 0.31 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.17 Pain Relevance 0

The pEYFP-hOGG1 plasmid was constructed by ligating the hOGG1 cDNA fragment from pEGFP-hOGG1 into the pEYFP-N1 vector.

hOGG1 Binding (ligating) of

8) Confidence 0.31 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

As shown in Figure 2B, EGFP-hOGG1 is associated with the nucleoli during S-phase.

EGFP-hOGG1 Binding (associated) of

9) Confidence 0.31 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

Interestingly, the anti-phosphoserine recognized a phosphorylated form of the enzyme in both EGFP-hOGG1 and EGFP-hOGG1-Glu326 chromatin fractions.

EGFP-hOGG1 Binding (recognized) of

10) Confidence 0.31 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.06

Thus, the nucleolus might be a sequestering compartment of hOGG1 to avoid the accumulation of GC?

hOGG1 Spec (might) Binding (compartment) of

11) Confidence 0.31 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

Finally, we wanted to examine whether EGFP-hOGG1-Glu326 would also be associated with the soluble chromatin and the nuclear matrix.

EGFP-hOGG1 Binding (associated) of

12) Confidence 0.30 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

Finally, by mimicking a hOGG1 protein phosphorylated at Ser-326 we demonstrate that the regulation of the nucleolar localization, soluble chromatin, nuclear matrix and condensed chromatin association of hOGG1 are mediated through the phosphorylation of Ser-326.

hOGG1 Binding (association) of

13) Confidence 0.30 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

EGFP (30 kDa), EGFP-hOGG1, EGFP-hOGG1-Cys326 and EGFP-hOGG1-Glu326 (68 kDa) were recognized by the anti-GFP specific antibody (Figure 3A) while only EGFP-hOGG1, EGFP-hOGG1-Cys326 and EGFP-hOGG1-Glu326 (68 kDa) were recognized by the anti-OGG1 specific antibody (Figure 3B).

EGFP-hOGG1 Binding (recognized) of

14) Confidence 0.30 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

Thus, compartmentalization of hOGG1 to the nucleoli during S-phase might increase the repair efficiency of A:8-oxoG mispairs that arise during DNA replication, as dCMP or dAMP can be selectively incorporated opposite 8-oxoG (7).

hOGG1 Spec (might) Binding (compartmentalization) of

15) Confidence 0.30 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

Thus, we suggest that the Ser-326 has to be phosphorylated to interact with a protein during S-phase that will translocate EGFP-hOGG1 to the nucleolus and to the condensed chromosomes during mitosis.

EGFP-hOGG1 Binding (interact) of

16) Confidence 0.30 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.08 Pain Relevance 0

In humans, 8-oxodG is repaired by 8-oxoguanine DNA glycosylase (OGG1), an enzyme that recognizes and hydrolyzes the aberrant base from the DNA backbone.

OGG1 Binding (recognizes) of

17) Confidence 0.28 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2910735 Disease Relevance 0.10 Pain Relevance 0

OGG1 is the DNA repair enzyme that recognizes and excises 8-oxodG [70].

OGG1 Binding (recognizes) of

18) Confidence 0.28 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2910735 Disease Relevance 0.32 Pain Relevance 0

In humans, 8-oxodG is repaired by 8-oxoguanine DNA glycosylase (OGG1), an enzyme that recognizes and hydrolyzes the aberrant base from the DNA backbone.

DNA glycosylase Binding (recognizes) of

19) Confidence 0.24 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2910735 Disease Relevance 0.11 Pain Relevance 0

They identified showed the presence of significant association between the Ser326Cys polymorphism of OGG1 and AML.

OGG1 (S326C) Binding (association) of associated with myeloid leukemia

20) Confidence 0.24 Published 2008 Journal Current Genomics Section Body Doc Link PMC2691673 Disease Relevance 0.83 Pain Relevance 0

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