INT182442

Context Info Confidence 0.58 First Reported 2005 Last Reported 2005 Negated 0 Speculated 1 Reported most in Body Documents 1 Total Number 5 Disease Relevance 0.07 Pain Relevance 0.15

nucleoplasm (OGG1) mitochondrion (OGG1) lyase activity (OGG1) hydrolase activity, acting on glycosyl bonds (OGG1) nucleolus (OGG1) nucleus (OGG1)

Anatomy Link Frequency HeLa S3 2 OGG1 (Homo sapiens)

Pain Link Frequency Relevance Heat imagery 5 87.28 Pain 5 74.96 Glutamate 5 24.04

Disease Link Frequency Relevance Heat Pain 5 74.96 Contagious Ecthyma 5 48.00 Cancer 45 5.00 Cockayne Syndrome 10 5.00 Disease 5 5.00 Aging 5 5.00 Adenoma 5 5.00 Stress 5 5.00 Colon Cancer 5 5.00 Carcinoma 5 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

To determine the distribution of EGFP-hOGG1-Cys326 during mitosis, we created stable cell lines overexpressing EGFP-hOGG1-Cys326.

Positive_regulation (overexpressing) of Gene_expression (overexpressing) of EGFP-hOGG1

1) Confidence 0.58 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

Cell-cycle-dependent expression of HOGG1 gene

Positive_regulation (dependent) of Gene_expression (expression) of HOGG1

2) Confidence 0.44 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.07 Pain Relevance 0.07

Overexpression of EGFP and EGFP-hOGG1 in the stable cell lines was examined in nuclear extracts by western blot using anti-OGG1 and anti-GFP specific antibodies.

Positive_regulation (Overexpression) of Spec (examined) Gene_expression (Overexpression) of EGFP-hOGG1

3) Confidence 0.42 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

We transiently co-transfected HeLa S3 cells with EYFP-hOGG1 and ECFP-PCNA and followed the cells for 20 h.

Positive_regulation (transfected) of Gene_expression (transfected) of EYFP-hOGG1 in HeLa S3

4) Confidence 0.42 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

Northern blot hybridization of synchronized HaCat cells showed a cell-cycle-dependent mRNA expression of hOGG1 with increased transcription of the major nuclear and mitochondrial isoforms during S/G2 (Figure 1).

Positive_regulation (dependent) of Gene_expression (expression) of hOGG1

5) Confidence 0.39 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.03

General Comments

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