INT184722

Context Info Confidence 0.47 First Reported 2005 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 3 Total Number 4 Disease Relevance 1.22 Pain Relevance 0.03

transport (Smad3) intracellular (Smad3) DNA binding (Smad3) protein complex (Smad3) cytoplasm (Smad3) nucleus (Smad3)

Anatomy Link Frequency parenchyma 1 Smad3 (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 26 60.48 fibrosis 15 36.96 cytokine 8 5.00 Inflammatory response 6 5.00 anesthesia 4 5.00 Calcitonin gene-related peptide 3 5.00 ketamine 2 5.00 Inflammatory stimuli 1 5.00 Inflammatory mediators 1 5.00 rheumatoid arthritis 1 5.00

Disease Link Frequency Relevance Heat Targeted Disruption 64 100.00 Cancer 3 87.92 Injury 26 72.92 Cytomegalovirus Infection 1 63.68 INFLAMMATION 33 61.20 Edema 3 56.56 Cataract 68 49.16 Fibrosis 13 36.96 Increased Venous Pressure Under Development 1 36.84 Sprains And Strains 3 11.52

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Increased immunoreactivity for Smad 3 in lung parenchyma of XRT-WT animals was noticeable at 1 week, and which steadily increased from 3–14 weeks.

Positive_regulation (Increased) of Smad 3 in parenchyma

1) Confidence 0.47 Published 2005 Journal BMC Cancer Section Body Doc Link PMC1177930 Disease Relevance 0.89 Pain Relevance 0.03

To identify which domains of Smad3 are required for the interaction, GST-pulldown assays were performed using deletion constructs for Smad3.

Positive_regulation (required) of Smad3

2) Confidence 0.36 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0

The pGEX-human Smad3 construct (Addgene plasmid 12630) encoding for GST-Smad3 was generated in the lab of Dr.

Positive_regulation (generated) of Smad3

3) Confidence 0.36 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0.09 Pain Relevance 0

Smad3 knockout mice were generated by the removal of exon 8 of the Smad3 gene in mice of background 129SV/EV x C57BL/6 [30].

Positive_regulation (generated) of Smad3 associated with targeted disruption

4) Confidence 0.35 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2647562 Disease Relevance 0.25 Pain Relevance 0

General Comments

This test has worked.