INT184763

Context Info Confidence 0.36 First Reported 2005 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 13 Total Number 13 Disease Relevance 3.93 Pain Relevance 0.21

Anatomy Mention Frequency Arm 1 cleavage 1

Pain Term Frequency Confidence Heat Arthritis 87 86.24 Neuropeptide 38 83.28 Dopamine 20 63.84 Inflammation 46 57.72 Freund adjuvant 1 49.32 cytokine 20 44.96 Glutamate 1 35.80 Central nervous system 41 34.28 Substantia nigra 9 16.24 cerebral cortex 3 5.00

Disease Term Frequency Confidence Heat Suicidal Behaviour 5 94.52 Contagious Ecthyma 8 94.24 Sprains And Strains 309 92.12 Cytomegalovirus Infection 5 89.84 Targeted Disruption 59 87.44 Reprotox - General 1 1 86.68 Arthritis 84 86.24 Rheumatoid Arthritis 81 86.12 Yersinia Infection 118 85.24 Bites And Stings 8 77.52

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Briefly, the profiling of whole genome micro-restriction fingerprints with EcoRI/MseI enzymes using fluorescence tagged primer pairs EcoRI+A/MseI+0 and EcoRI+G or A / MseI+0 was performed.

EcoRI Binding (fingerprints) of

1) Confidence 0.36 Published 2005 Journal BMC Microbiol Section Body Doc Link PMC1180443 Disease Relevance 0.24 Pain Relevance 0

This formed a 98-bp fragment that was ligated into EcoRI- and XbaI-digested WT human a-Syn in pcDNA3.1.

EcoRI Binding (ligated) of

2) Confidence 0.36 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878529 Disease Relevance 0.34 Pain Relevance 0.03

The product with overhanging SalI and EcoRI ends was subsequently cloned into the respective sites in pGEM-Teasy plasmid (Promega Corporation, Madison, WI, USA) and transformed into Escherichia coli (DH5?)

EcoRI Binding (ends) of

3) Confidence 0.36 Published 2010 Journal Veterinary Research Section Body Doc Link PMC2826093 Disease Relevance 0 Pain Relevance 0

The amplified product was cloned in a polylinker-containing vector, excised with EcoRI and BamI, and subcloned into pCMV2/FLAG (Sigma-Aldrich, St.

EcoRI Binding (excised) of

4) Confidence 0.36 Published 2009 Journal Mol Cancer Section Body Doc Link PMC2776015 Disease Relevance 0.31 Pain Relevance 0

The construct's Left Arm was extracted from the template with EcoRI and HincII, generating a 1.1 kbp into Intron 5 up to the corresponding HincII site, 5?

EcoRI Binding (template) of in Arm

5) Confidence 0.36 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2774945 Disease Relevance 0.23 Pain Relevance 0

PCR product was digested with XhoI and EcoRI and ligated into XhoI/EcoRI-digested pcDNA3.1 vectors (Invitrogen, Carlsbad, CA).

EcoRI Binding (ligated) of

6) Confidence 0.36 Published 2009 Journal PLoS Medicine Section Body Doc Link PMC2760136 Disease Relevance 0.90 Pain Relevance 0.07

A linear lacZ-U-cat-sacB-TT araC PBAD spoT-lacZ-D fragment was purified from plasmid pYA4575 by digestion with EcoRI and SphI and transformed into ?

EcoRI Binding (digestion) of

7) Confidence 0.36 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2726946 Disease Relevance 0.51 Pain Relevance 0

C. glabrata genomic DNA was used as a template to amplify by PCR CgPDR1 using the primers CgPDR1-EcoRI (5?

EcoRI Binding (primers) of

8) Confidence 0.36 Published 2009 Journal PLoS Pathogens Section Body Doc Link PMC2607542 Disease Relevance 0.23 Pain Relevance 0

POMC plasmid was linearized with EcoRI, and T3 polymerase was used for in vitro transcription to generate antisense cRNA.

EcoRI Binding (linearized) of

9) Confidence 0.36 Published 2008 Journal Diabetes Section Body Doc Link PMC2494674 Disease Relevance 0.19 Pain Relevance 0

end by EcoRI and cloned into the 3?

EcoRI Binding (end) of

10) Confidence 0.36 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2396793 Disease Relevance 0 Pain Relevance 0

(nucleotides 562 to 585) (EcoRI cleavage site underlined) and DA152 (5?

EcoRI Binding (underlined) of in cleavage

11) Confidence 0.36 Published 2007 Journal PLoS Medicine Section Body Doc Link PMC2071934 Disease Relevance 0.45 Pain Relevance 0

digestion by EcoRI and then cloned into pSS2 digested by

EcoRI Binding (digestion) of

12) Confidence 0.36 Published 2007 Journal Journal of Biomedicine and Biotechnology Section Body Doc Link PMC1940057 Disease Relevance 0.52 Pain Relevance 0

The PCR fragment was subsequently digested with AgeI and EcoRI and ligated into AgeI- EcoRI digested pCMV-VWF-?

EcoRI Binding (digested) of

13) Confidence 0.36 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2928777 Disease Relevance 0 Pain Relevance 0.11

General Comments

This test has worked.