INT184763
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Briefly, the profiling of whole genome micro-restriction fingerprints with EcoRI/MseI enzymes using fluorescence tagged primer pairs EcoRI+A/MseI+0 and EcoRI+G or A / MseI+0 was performed. | |||||||||||||||
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This formed a 98-bp fragment that was ligated into EcoRI- and XbaI-digested WT human a-Syn in pcDNA3.1. | |||||||||||||||
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The product with overhanging SalI and EcoRI ends was subsequently cloned into the respective sites in pGEM-Teasy plasmid (Promega Corporation, Madison, WI, USA) and transformed into Escherichia coli (DH5?) | |||||||||||||||
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The amplified product was cloned in a polylinker-containing vector, excised with EcoRI and BamI, and subcloned into pCMV2/FLAG (Sigma-Aldrich, St. | |||||||||||||||
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The construct's Left Arm was extracted from the template with EcoRI and HincII, generating a 1.1 kbp into Intron 5 up to the corresponding HincII site, 5? | |||||||||||||||
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PCR product was digested with XhoI and EcoRI and ligated into XhoI/EcoRI-digested pcDNA3.1 vectors (Invitrogen, Carlsbad, CA).
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A linear lacZ-U-cat-sacB-TT araC PBAD spoT-lacZ-D fragment was purified from plasmid pYA4575 by digestion with EcoRI and SphI and transformed into ? | |||||||||||||||
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C. glabrata genomic DNA was used as a template to amplify by PCR CgPDR1 using the primers CgPDR1-EcoRI (5? | |||||||||||||||
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POMC plasmid was linearized with EcoRI, and T3 polymerase was used for in vitro transcription to generate antisense cRNA. | |||||||||||||||
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end by EcoRI and cloned into the 3? | |||||||||||||||
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(nucleotides 562 to 585) (EcoRI cleavage site underlined) and DA152 (5? | |||||||||||||||
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digestion by EcoRI and then cloned into pSS2 digested by | |||||||||||||||
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The PCR fragment was subsequently digested with AgeI and EcoRI and ligated into AgeI- EcoRI digested pCMV-VWF-? | |||||||||||||||
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General Comments
This test has worked.