INT184844

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Context Info
Confidence 0.78
First Reported 2005
Last Reported 2011
Negated 8
Speculated 0
Reported most in Body
Documents 130
Total Number 133
Disease Relevance 14.32
Pain Relevance 12.13

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

endosome (Snap25) plasma membrane (Snap25) cytoplasm (Snap25)
Anatomy Link Frequency
brain 12
neurons 11
neuronal 6
adult brain 4
granule cells 4
Snap25 (Mus musculus)
Pain Link Frequency Relevance Heat
GABAergic 2433 99.84 Very High Very High Very High
Thalamus 220 99.52 Very High Very High Very High
Neurotransmitter 828 99.36 Very High Very High Very High
Glutamate 152 99.08 Very High Very High Very High
Cholecystokinin 336 98.84 Very High Very High Very High
Pyramidal cell 571 98.76 Very High Very High Very High
Dopamine 158 98.56 Very High Very High Very High
nMDA receptor 11 98.36 Very High Very High Very High
Calcium channel 432 96.60 Very High Very High Very High
gABA 57 96.44 Very High Very High Very High
Disease Link Frequency Relevance Heat
Targeted Disruption 624 99.96 Very High Very High Very High
Embryonic Lethality 56 99.36 Very High Very High Very High
Cognitive Disorder 411 98.88 Very High Very High Very High
Convulsion 728 98.08 Very High Very High Very High
Motor Neuron Diseases 107 97.52 Very High Very High Very High
Diabetes Mellitus 310 97.20 Very High Very High Very High
Sprains And Strains 206 96.96 Very High Very High Very High
Ataxia 58 96.28 Very High Very High Very High
Aging 8 95.20 Very High Very High Very High
Retina Disease 56 93.20 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Unexpectedly, despite that neo-containing SNAP-25b deficient mutants express only 50% of normal SNAP-25 mRNA levels, protein levels of SNAP-25 are only reduced to 80% of WT littermates.


Gene_expression (express) of SNAP-25 mRNA
1) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.08 Pain Relevance 0
Separate overexpression of the two SNAP-25 isoforms in embryonic adrenal chromaffin cells from these SNAP-25 knock-out (KO) mice showed that the SNAP-25b isoform had a higher capability to stabilize the pool of primed vesicles than SNAP-25a, since the burst of Ca2+-evoked catecholamine release differed [28].
Gene_expression (overexpression) of SNAP-25 in vesicles associated with targeted disruption and catecholamine
2) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.47 Pain Relevance 0.05
Together, our results demonstrate that homozygous neo-containing SNAP-25b deficient mouse mutants only express SNAP-25a and that total SNAP-25 mRNA and protein levels are reduced.
Gene_expression (express) of SNAP-25a
3) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.07 Pain Relevance 0
At PN14, SNAP-25b levels were five times higher than SNAP-25a in WT mice, heterozygous neo-containing mutants had equal amounts of both SNAP-25 mRNA isoforms, while homozygous neo-containing SNAP-25b deficient mutants only expressed SNAP-25a (Figure 1C).
Gene_expression (expressed) of SNAP-25a
4) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0 Pain Relevance 0
At physiological levels of SNAP-25 expression and elevated Ca2+-concentrations the replacement of SNAP-25b by SNAP-25a does not affect synaptic release enough to result in a statistically significant effect.
Gene_expression (expression) of SNAP-25
5) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.14 Pain Relevance 0
To develop mouse mutants that only express SNAP-25a, a gene targeting vector with an additional exon 5a sequence, replacing the mouse exon 5b sequence, was generated (Figure 1A).
Gene_expression (express) of SNAP-25a
6) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.20 Pain Relevance 0
The neo-excised SNAP-25b deficient mutants, with restored levels of SNAP-25 expression, escaped the critical early PN period with minor defects.
Gene_expression (expression) of SNAP-25
7) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.17 Pain Relevance 0.05
Elimination of SNAP-25b expression resulted in developmental defects, spontaneous seizures, and impaired short-term synaptic plasticity.
Gene_expression (expression) of SNAP-25b associated with convulsion
8) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Abstract Doc Link PMC2581893 Disease Relevance 0.10 Pain Relevance 0.09
Our results show, that homozygous neo-containing SNAP-25b deficient mice, lacking SNAP-25b expression and having a moderate reduction in total levels of SNAP-25 protein, exhibit severe developmental and behavioral defects.


Gene_expression (expression) of SNAP-25b
9) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.16 Pain Relevance 0
In mouse brain, SNAP-25a precedes SNAP-25b expression during development, but by the second postnatal (PN) week SNAP-25b becomes the major splice variant, concomitantly with a dramatic increase in SNAP-25 expression [26].
Gene_expression (expression) of SNAP-25 in brain
10) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.24 Pain Relevance 0.03
In higher vertebrates SNAP-25 is expressed as two developmentally regulated and complementary distributed splice variants termed SNAP-25a and SNAP-25b [23].
Gene_expression (expressed) of SNAP-25
11) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0 Pain Relevance 0
The exon 5b was genetically eliminated and replaced with a second supplementary exon 5a, to maintain alternative splicing and normal expression levels, but allowing only SNAP-25a to be expressed.
Gene_expression (expressed) of SNAP-25a
12) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.65 Pain Relevance 0.04
Thus, we have developed a SNAP-25b deficient mouse mutant by replacing exon 5b with an additional exon 5a equivalent, thereby preventing expression of SNAP-25b but not the alternative splicing.


Gene_expression (expression) of SNAP-25b
13) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.08 Pain Relevance 0
Initially, we therefore investigated the level of SNAP-25 mRNA and protein expression in brain of neo-containing SNAP-25b deficient mutants and wild-type (WT) littermates at PN14.
Gene_expression (expression) of SNAP-25 mRNA in brain
14) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.07 Pain Relevance 0
The reduced SNAP-25 expression in neo-containing SNAP-25b deficient mutants could be due to the presence of the Tkneo selection gene.
Gene_expression (expression) of SNAP-25
15) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.14 Pain Relevance 0
In neo-excised mutants SNAP-25 protein expression was moderately but significantly increased to 111% compared to WT (Figure 2D).
Gene_expression (expression) of SNAP-25 protein
16) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.25 Pain Relevance 0
SNAP-25b is normally highly expressed in brain areas involved in cognitive function, such as the HF.
Gene_expression (expressed) of SNAP-25b in brain associated with cognitive disorder
17) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.28 Pain Relevance 0.04
We now show that expression of SNAP-25b is important for early postnatal development and synaptic plasticity.
Gene_expression (expression) of SNAP-25b
18) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.08 Pain Relevance 0
Unexpectedly, despite that neo-containing SNAP-25b deficient mutants express only 50% of normal SNAP-25 mRNA levels, protein levels of SNAP-25 are only reduced to 80% of WT littermates.


Gene_expression (express) of SNAP-25b
19) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.08 Pain Relevance 0
In mouse brain, SNAP-25a precedes SNAP-25b expression during development, but by the second postnatal (PN) week SNAP-25b becomes the major splice variant, concomitantly with a dramatic increase in SNAP-25 expression [26].
Gene_expression (expression) of SNAP-25b in brain
20) Confidence 0.78 Published 2008 Journal PLoS Genetics Section Body Doc Link PMC2581893 Disease Relevance 0.20 Pain Relevance 0

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