INT185258

Context Info Confidence 0.60 First Reported 2005 Last Reported 2011 Negated 9 Speculated 0 Reported most in Body Documents 33 Total Number 41 Disease Relevance 11.28 Pain Relevance 0.70

cytosol (Pou5f1) nucleoplasm (Pou5f1) nucleus (Pou5f1) DNA binding (Pou5f1) transcription factor binding (Pou5f1) cytoplasm (Pou5f1)

Anatomy Link Frequency stem cell 8 brain 3 upper 3 blood 2 mesoderm 2 Pou5f1 (Mus musculus)

Pain Link Frequency Relevance Heat Potency 14 99.06 antagonist 2 93.48 Inflammatory mediators 14 93.32 ischemia 106 85.76 Action potential 66 79.28 imagery 113 77.40 anesthesia 27 17.28 isoflurane 18 16.00 Central nervous system 107 5.00 neurotrophin 3 72 5.00

Disease Link Frequency Relevance Heat Infection 438 99.62 Cytomegalovirus Infection 504 99.42 Cancer 250 98.68 Obesity 126 97.34 Glioblastoma 18 94.40 Teratoma 121 93.92 INFLAMMATION 50 92.92 Injury 132 92.76 Tendinopathy 84 91.52 Malignant Neoplastic Disease 15 91.20

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Mutation

Event

Anatomy

Negation

Speculation

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Disease term

In contrast, colonies of undifferentiated ESCs and high expression levels of Nanog and Pou5f1 were found in cultures of the sorted GFP(?)

Gene_expression (expression) of Pou5f1

1) Confidence 0.60 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2902505 Disease Relevance 0.34 Pain Relevance 0

We have previously demonstrated that no expression of Nanog or Pou5f1 was detected following Nkx2-5(+) CPC derivation and extended culture in vitro.

Neg (no) Gene_expression (expression) of Pou5f1

2) Confidence 0.60 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2902505 Disease Relevance 0.37 Pain Relevance 0

No colonies of undifferentiated ESCs were detected as confirmed by the absence of Pou5f1 and Nanog expression.

Gene_expression (expression) of Pou5f1

3) Confidence 0.54 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2902505 Disease Relevance 0 Pain Relevance 0.04

Oct4 expression must be closely regulated; too much or too little will actually induce differentiation [26].

Gene_expression (expression) of Oct4

4) Confidence 0.51 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3020957 Disease Relevance 0.06 Pain Relevance 0

and CD24(hi)CD29(+) cells, isolated from uninfected control brains, expressed Oct4 (Fig. 6, upper panel, histogram with blue line).

Gene_expression (expressed) of Oct4 in upper

5) Confidence 0.51 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3020957 Disease Relevance 0.15 Pain Relevance 0

To determine if CD24(hi) cells from the developing brain expressed Oct4, we performed intracellular staining for Oct4 as well as surface stained for CD15, CD24 and CD29 and analyzed these cell populations using flow cytometry.

Gene_expression (expressed) of Oct4 in brain

6) Confidence 0.51 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3020957 Disease Relevance 0.10 Pain Relevance 0

We then went on to determine if MCMV infection had any effect on Oct4 expression among this defined subset of cells.

Gene_expression (expression) of Oct4 associated with cytomegalovirus infection and infection

7) Confidence 0.51 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3020957 Disease Relevance 0.38 Pain Relevance 0

negative cells were found to express Oct4, it appeared that once CD29 expression occurred on these CD24(hi) cells the number of Oct4-expressing cells was reduced (Fig. 6, upper panel, histogram on the right).

Gene_expression (express) of Oct4 in upper

8) Confidence 0.51 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3020957 Disease Relevance 0.17 Pain Relevance 0

Data obtained from these experiments demonstrated that Oct4 expression was reduced in cells obtained from virus-infected brains compared to the cells from the brains of uninfected animals (upper panel, histogram overlays).

Gene_expression (expression) of Oct4 in brains

9) Confidence 0.51 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3020957 Disease Relevance 0.49 Pain Relevance 0

In our microarray analysis, we observed high expression of pluripotency-related genes involved in the core hESC regulatory circuitry, including OCT4, SOX2, and NANOG, as well as CRYPTO 1 and 3, LCK, and HESX1.

Gene_expression (expression) of OCT4

10) Confidence 0.49 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565131 Disease Relevance 0.18 Pain Relevance 0

In contrast, colonies of undifferentiated ESCs and high expression levels of Nanog and Pou5f1 were found in cultures of the sorted GFP(?)

Gene_expression (levels) of Pou5f1

11) Confidence 0.47 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2902505 Disease Relevance 0.34 Pain Relevance 0

Altered expression of the Oct4 transcription factor

Gene_expression (expression) of Oct4

12) Confidence 0.44 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3020957 Disease Relevance 0.07 Pain Relevance 0

Reverse transcription polymerase chain reaction (RT-PCR) was used to compare the expression of embryonic markers (Oct4, NANOG, Rex1), cardiac transcription factors (Nkx2.5, MEF2C), ventricular specific proteins (?

Gene_expression (expression) of Oct4

13) Confidence 0.42 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565131 Disease Relevance 0 Pain Relevance 0

The pluripotency marker OCT3/4 (POU5F1) was not found to be expressed (Table 3).

Gene_expression (expressed) of POU5F1

14) Confidence 0.42 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440803 Disease Relevance 1.21 Pain Relevance 0

NS cells do not express pluripotent cell-specific transcription factors Oct-4 and Nanog, but show appropriate expression of neural genes and absence of mesoderm and endoderm-specific genes.

Neg (not) Gene_expression (express) of Oct-4 in endoderm

15) Confidence 0.41 Published 2005 Journal PLoS Biology Section Body Doc Link PMC1184591 Disease Relevance 0.06 Pain Relevance 0

negative cells were found to express Oct4, it appeared that once CD29 expression occurred on these CD24(hi) cells the number of Oct4-expressing cells was reduced (Fig. 6, upper panel, histogram on the right).

Gene_expression (expressing) of Oct4 in upper

16) Confidence 0.40 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3020957 Disease Relevance 0.28 Pain Relevance 0

This neonatal brain infection was also associated with altered expression of Oct4, a multipotency marker; as well as down regulation of the neurotrophins BDNF and NT3, which are essential to regulate the birth and differentiation of neurons during normal brain development.

Gene_expression (expression) of Oct4 in brain associated with infection

17) Confidence 0.40 Published 2011 Journal PLoS ONE Section Abstract Doc Link PMC3020957 Disease Relevance 1.59 Pain Relevance 0

Expression of stem cell markers (Oct4, NANOG, Rex1) decreased substantially by day 28, while early stage cardiac transcriptional factors (Nkx2.5, MEF2C) appeared between days 14–28.

Gene_expression (Expression) of Oct4 in stem cell

18) Confidence 0.38 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565131 Disease Relevance 0.09 Pain Relevance 0.12

These cells lack detectable expression of the pluripotency factors Oct4 and Nanog, and also of the early neural marker Sox1, but retain the pan-neuroepithelial marker Sox2 (Part r in Figure 2C).

Gene_expression (expression) of Oct4 in neural

19) Confidence 0.36 Published 2005 Journal PLoS Biology Section Body Doc Link PMC1184591 Disease Relevance 0 Pain Relevance 0

NS cells do not express pluripotent cell-specific transcription factors Oct-4 and Nanog, but show appropriate expression of neural genes and absence of mesoderm and endoderm-specific genes.

Neg (not) Gene_expression (express) of Oct-4 in endoderm

20) Confidence 0.35 Published 2005 Journal PLoS Biology Section Body Doc Link PMC1184591 Disease Relevance 0.06 Pain Relevance 0

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