INT186116

Context Info Confidence 0.44 First Reported 2005 Last Reported 2010 Negated 6 Speculated 1 Reported most in Body Documents 9 Total Number 10 Disease Relevance 5.85 Pain Relevance 2.32

nucleoplasm (MAPK1) mitochondrion (MAPK1) Golgi apparatus (MAPK1) DNA binding (MAPK1) protein complex (MAPK1) response to stress (MAPK1)

Anatomy Link Frequency colon 2 MAPK1 (Homo sapiens)

Pain Link Frequency Relevance Heat bradykinin 300 97.24 antagonist 30 93.60 agonist 12 92.44 Cannabinoid receptor 96 92.12 Inflammation 183 89.56 Osteoarthritis 267 87.84 rheumatoid arthritis 297 87.56 imagery 23 82.76 Endocannabinoid 129 62.64 Cannabinoid 15 52.08

Disease Link Frequency Relevance Heat Colon Cancer 50 99.40 Targeted Disruption 19 97.72 Stress 408 97.52 INFLAMMATION 179 89.56 Frailty 270 87.84 Rheumatoid Arthritis 297 87.56 Disease 813 84.16 Death 24 82.36 Apoptosis 166 81.92 Prostate Cancer 27 50.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Total cellular levels of ERK, JNK, and p38 proteins did not change significantly over the time courses of up to 1 hour incubation with BK or H2O2.

Neg (not) Regulation (change) of Gene_expression (levels) of ERK associated with bradykinin

1) Confidence 0.44 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2644820 Disease Relevance 0 Pain Relevance 0.09

Cell lysates collected in 1% SDS were immunoblotted for expression and activation of ERK, JNK and p38 and normalized to the loading control protein tubulin.

Regulation (immunoblotted) of Gene_expression (expression) of ERK

2) Confidence 0.32 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2644820 Disease Relevance 1.36 Pain Relevance 0.25

Total cellular levels of ERK, JNK, and p38 proteins did not change significantly over the time courses of up to 1 hour incubation with BK or H2O2.

Neg (not) Regulation (change) of Gene_expression (levels) of p38 associated with bradykinin

3) Confidence 0.32 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2644820 Disease Relevance 0 Pain Relevance 0.09

Cell lysates collected in 1% SDS were immunoblotted for expression and activation of ERK, JNK and p38 and normalized to the loading control protein tubulin.

Regulation (immunoblotted) of Gene_expression (expression) of p38

4) Confidence 0.32 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2644820 Disease Relevance 1.42 Pain Relevance 0.25

Levels of total ERK1, ERK2, and p38 were unaffected by HU210 (data not shown).

Neg (unaffected) Regulation (unaffected) of Gene_expression (Levels) of ERK2

5) Confidence 0.26 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2453762 Disease Relevance 0.86 Pain Relevance 0.58

Levels of total ERK1, ERK2, and p38 were unaffected by HU210 (data not shown).

Neg (unaffected) Regulation (unaffected) of Gene_expression (Levels) of p38

6) Confidence 0.26 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2453762 Disease Relevance 0.86 Pain Relevance 0.58

Levels of total ERK1 and ERK2 were unaffected by the drug treatments (Figure 7a).

Neg (unaffected) Regulation (unaffected) of Gene_expression (Levels) of ERK2

7) Confidence 0.26 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2453762 Disease Relevance 0.26 Pain Relevance 0.44

There was no effect of drug or EGF treatment on total ERK1/2 expression.

Neg (no) Regulation (effect) of Gene_expression (expression) of ERK1/2

8) Confidence 0.20 Published 2005 Journal J Carcinog Section Body Doc Link PMC1208922 Disease Relevance 0.35 Pain Relevance 0

In order to verify that EGFR signaling was intact in our cells, the effects of EGF treatment on the expression and activation of EGFR and ERK1/2 in HT29 human colon cancer cells was examined.

Spec (examined) Regulation (effects) of Gene_expression (expression) of ERK1/2 in colon associated with colon cancer

9) Confidence 0.12 Published 2005 Journal J Carcinog Section Body Doc Link PMC1208922 Disease Relevance 0.26 Pain Relevance 0.04

We then calculated the effect of these perturbation on cyclin D and PSA expression levels along with ERK and AR activation levels.

Regulation (effect) of Gene_expression (expression) of ERK

10) Confidence 0.06 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2812491 Disease Relevance 0.49 Pain Relevance 0

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