INT186286

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Context Info
Confidence 0.79
First Reported 2005
Last Reported 2009
Negated 1
Speculated 0
Reported most in Body
Documents 18
Total Number 19
Disease Relevance 5.07
Pain Relevance 1.61

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

signal transduction (Stat6) nucleus (Stat6) DNA binding (Stat6)
cytoplasm (Stat6) signal transducer activity (Stat6)
Anatomy Link Frequency
embryos 3
bands 3
lung 3
nucleus 1
mast cells 1
Stat6 (Mus musculus)
Pain Link Frequency Relevance Heat
cytokine 467 99.74 Very High Very High Very High
chemokine 17 96.52 Very High Very High Very High
Inflammation 130 93.08 High High
tolerance 1 64.64 Quite High
Pain 16 54.64 Quite High
Inflammatory mediators 8 48.32 Quite Low
Arthritis 8 40.00 Quite Low
Inflammatory response 8 35.00 Quite Low
Hyperalgesia 3 22.24 Low Low
withdrawal 1 18.32 Low Low
Disease Link Frequency Relevance Heat
Hyperplasia 6 98.40 Very High Very High Very High
Candida Infection 274 98.24 Very High Very High Very High
Asthma 349 95.76 Very High Very High Very High
Pneumonia 8 93.08 High High
Apoptosis 13 92.44 High High
Rhinitis 8 92.36 High High
Targeted Disruption 73 89.60 High High
Disease 65 89.48 High High
INFLAMMATION 139 88.72 High High
Hypersensitivity 105 87.92 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
An appropriate size band for phosphorylated STAT-6 is seen in both positive control lanes.
Phosphorylation (phosphorylated) of STAT-6 in band
1) Confidence 0.79 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0 Pain Relevance 0
However, phosphorylated STAT-6 persisted much longer with a more prolonged exposure to IL-4 [24].
Phosphorylation (phosphorylated) of STAT-6
2) Confidence 0.79 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0.18 Pain Relevance 0.04
In cell culture studies, the half-life of phosphorylated STAT-6 after a single bolus dose of IL-4 was less then one hour.
Phosphorylation (phosphorylated) of STAT-6
3) Confidence 0.79 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0.19 Pain Relevance 0.05
A second potential issue is the relatively short half-life of phosphorylated STAT-6.
Phosphorylation (phosphorylated) of STAT-6
4) Confidence 0.79 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0.19 Pain Relevance 0.05
This seems unlikely as the positive controls (both embryo and adult) still showed phosphorylation of STAT-6 at the same 1 hour time point.
Phosphorylation (phosphorylation) of STAT-6 in embryo
5) Confidence 0.79 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0 Pain Relevance 0.09
In the 3 experimental lanes, there were no detectable phosphorylated STAT-6 bands.
Neg (no) Phosphorylation (phosphorylated) of STAT-6 in bands
6) Confidence 0.79 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0 Pain Relevance 0
A 3rd possible problem is the sensitivity of Western Blot for detection of phosphorylated -STAT-6.
Phosphorylation (phosphorylated) of STAT-6
7) Confidence 0.79 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0 Pain Relevance 0.13
It is possible that even if transplacental passage of IL-4 or 13 occurred, phosphorylated STAT-6 may have already degraded by the time the embryos were harvested, leading to a false negative.
Phosphorylation (phosphorylated) of STAT-6 in embryos
8) Confidence 0.79 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0.16 Pain Relevance 0.04
In negative control lanes, no bands were detected for phosphorylated STAT-6 (Figure 1).
Phosphorylation (phosphorylated) of STAT-6 in bands
9) Confidence 0.69 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0 Pain Relevance 0
We were unable to clearly and consistently detect phosphorylation of STAT-6 in lung tissue total protein extracts of directly injected (intraperitoneal injections) embryos (either wildtype or heterozygote) (not shown).
Phosphorylation (phosphorylation) of STAT-6 in embryos
10) Confidence 0.61 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0 Pain Relevance 0.03
To assess transplacental passage of functional cytokines, we assayed phosphorylation of STAT-6, a marker of IL-4 and -13 signaling via heterodimeric receptor complexes which require an IL-4 receptor alpha subunit.
Phosphorylation (phosphorylation) of STAT-6 associated with cytokine
11) Confidence 0.61 Published 2009 Journal PLoS ONE Section Abstract Doc Link PMC2645503 Disease Relevance 0.28 Pain Relevance 0.10
Following binding, STAT6 is phosphorylated, dimerizes, and translocates to the nucleus.
Phosphorylation (phosphorylated) of STAT6 in nucleus
12) Confidence 0.61 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0.50 Pain Relevance 0.19
Disruption of the IL-4Ra gene can prevent the phosphorylation of STAT-6 caused by either IL-4 or 13.
Phosphorylation (phosphorylation) of STAT-6
13) Confidence 0.61 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0.48 Pain Relevance 0.16
In order to increase our ability to detect phosphorylation of STAT-6, nuclear protein extracts from lung tissue were used.
Phosphorylation (phosphorylation) of STAT-6 in lung
14) Confidence 0.61 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0 Pain Relevance 0.03
Using nuclear protein extracts from lung tissue, phosphorylation of STAT-6 could be detected following injection of IL-4 into adult wild-type mice (R.O.) and fetal (I.P.)
Phosphorylation (phosphorylation) of STAT-6 in lung
15) Confidence 0.61 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2645503 Disease Relevance 0 Pain Relevance 0
Bulanova et al. [25] have shown that addition of extracellular ATP in the millimolar range induced activation of P2X7 expressed on murine mast cells, resulting in the rapid but transient phosphorylation of ERK, Jak2 and STAT6.
Phosphorylation (phosphorylation) of STAT6 in mast cells
16) Confidence 0.54 Published 2007 Journal Purinergic Signal Section Body Doc Link PMC2096758 Disease Relevance 0.71 Pain Relevance 0.30
Phosphorylated and homodimerized STAT6 binds to STAT binding elements (SBE), which includes GAS, and either trans-activates or inhibits transcription of the adjacent genes [212].
Phosphorylation (Phosphorylated) of STAT6
17) Confidence 0.39 Published 2005 Journal Virol J Section Body Doc Link PMC1215526 Disease Relevance 0.11 Pain Relevance 0.15
In addition, phosphorylation of STAT6 in the lung was more potently found in the CSBG + OVA than in the other groups (evidenced by Western blot analysis: data not shown).
Phosphorylation (phosphorylation) of STAT6 in lung associated with candida infection
18) Confidence 0.31 Published 2009 Journal Respir Res Section Body Doc Link PMC2731726 Disease Relevance 1.17 Pain Relevance 0.22
Moreover, we have previously found that a single intratracheal administration of CSBG can induce mucus hyperplasia, one of the consequences of the Th2 (IL-13)-dominant response and the enhanced phosphorylation of STAT6, one of the pivotal transcriptional factors in the Th2 milieu [42].
Phosphorylation (phosphorylation) of STAT6 associated with hyperplasia and candida infection
19) Confidence 0.31 Published 2009 Journal Respir Res Section Body Doc Link PMC2731726 Disease Relevance 1.12 Pain Relevance 0.03

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