INT188444

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Context Info
Confidence 0.44
First Reported 2005
Last Reported 2010
Negated 0
Speculated 1
Reported most in Body
Documents 5
Total Number 20
Disease Relevance 4.69
Pain Relevance 0.54

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (SQSTM1) cytosol (SQSTM1) nucleoplasm (SQSTM1)
nucleus (SQSTM1) response to stress (SQSTM1) cytoplasm (SQSTM1)
Anatomy Link Frequency
HeLa 3
knee 1
SQSTM1 (Homo sapiens)
Pain Link Frequency Relevance Heat
Osteoarthritis 59 94.40 High High
Pain 52 81.32 Quite High
Glutamate 15 38.56 Quite Low
Inflammatory response 11 5.00 Very Low Very Low Very Low
cytokine 9 5.00 Very Low Very Low Very Low
imagery 8 5.00 Very Low Very Low Very Low
Nerve growth factor 2 5.00 Very Low Very Low Very Low
Inflammation 2 5.00 Very Low Very Low Very Low
tolerance 2 5.00 Very Low Very Low Very Low
Arthritis 2 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Infection 5 99.86 Very High Very High Very High
Obesity 28 99.76 Very High Very High Very High
Disease 129 99.64 Very High Very High Very High
Measles 3 99.54 Very High Very High Very High
Bone Disease 218 99.20 Very High Very High Very High
Repression 15 98.72 Very High Very High Very High
Viral Infection 1 96.00 Very High Very High Very High
Knee Osteoarthritis 30 94.40 High High
Adenocarcinoma 30 83.68 Quite High
Pain 52 81.32 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
SQSTM1 binds to the bottom side of the six-bladed ?
SQSTM1 Binding (binds) of
1) Confidence 0.44 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.14 Pain Relevance 0
This has led to the suggestion that environmental factors such as measles virus infection might interact with SQSTM1 mutations to cause PDB [11].
SQSTM1 Spec (might) Binding (interact) of associated with measles, bone disease and infection
2) Confidence 0.37 Published 2008 Journal BMC Health Serv Res Section Body Doc Link PMC2442429 Disease Relevance 1.33 Pain Relevance 0
These findings suggest that PDB-related SQSTM1 mutations may confer a higher susceptibility to development of the disease by impairing the binding of the p62 protein to a ubiquitinated target.
p62 protein Binding (binding) of associated with disease and bone disease
3) Confidence 0.37 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1297578 Disease Relevance 0.48 Pain Relevance 0
The p62/SQSTM1 protein binds non-covalently to ubiquitin, co-localizing with ubiquitinated inclusions in several human diseases characterized by altered protein aggregation [10].
SQSTM1 Binding (binds) of associated with disease
4) Confidence 0.35 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1297578 Disease Relevance 0.40 Pain Relevance 0
Here, we have used immunopurification of Keap1 and mass spectrometry, in addition to immunoblotting, to identify sequestosome 1 (SQSTM1) as a cellular binding partner of Keap1.
SQSTM1 Binding (binding) of
5) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Abstract Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
UBA SQSTM1-FLAG/His mutant lacking the ubiquitin-associated domain was also capable of binding endogenous Keap1 in HEK293T and HeLa cells (Fig. 1B), demonstrating that the interaction between Keap1 and SQSTM1 was not dependent on the presence of the ubiquitin-associated domain of SQSTM1 and implying that the ubiquitination of Keap1 is not required for its association with SQSTM1.
SQSTM1 Binding (interaction) of in HeLa
6) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
Endogenous Keap1 was identified as a co-precipitating protein (Fig. 1C), demonstrating that SQSTM1 is a binding partner of Keap1 under physiological conditions.


SQSTM1 Binding (binding) of
7) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.24 Pain Relevance 0
UBA SQSTM1-FLAG/His mutant lacking the ubiquitin-associated domain was also capable of binding endogenous Keap1 in HEK293T and HeLa cells (Fig. 1B), demonstrating that the interaction between Keap1 and SQSTM1 was not dependent on the presence of the ubiquitin-associated domain of SQSTM1 and implying that the ubiquitination of Keap1 is not required for its association with SQSTM1.
SQSTM1 Binding (association) of in HeLa
8) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
In summary, this study has presented evidence for a physical and functional interaction between Keap1 and SQSTM1 in mammalian cells.
SQSTM1 Binding (interaction) of
9) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.43 Pain Relevance 0.10
This raised the possibility that SQSTM1 was a binding partner of Keap1.
SQSTM1 Binding (binding) of
10) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
These findings demonstrate a physical and functional interaction between Keap1 and SQSTM1 and reveal an additional layer of regulation in the Keap1-Nrf2 pathway.



SQSTM1 Binding (interaction) of
11) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Abstract Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
To investigate the biological significance of the interaction between Keap1 and SQSTM1, we examined the effect on Keap1 of depleting endogenous SQSTM1, using siRNA, or ectopically expressing SQSTM1-FLAG/His in a panel of human cell lines originating from discrete organs.
SQSTM1 Binding (interaction) of
12) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.31 Pain Relevance 0
Here, we have used immunopurification of Keap1 and mass spectrometry, in addition to immunoblotting, to identify sequestosome 1 (SQSTM1) as a cellular binding partner of Keap1.
sequestosome 1 Binding (binding) of
13) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Abstract Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
B activity (22), and we have shown here that depletion of SQSTM1 is associated with an induction of Keap1 and repression of Nrf2.
SQSTM1 Binding (associated) of associated with repression
14) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.17 Pain Relevance 0
In a very recent report (23), Komatsu and colleagues have demonstrated an interaction between Keap1 and SQSTM1 and have defined the motifs in Keap1 and SQSTM1 responsible for this interaction.
SQSTM1 Binding (interaction) of
15) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.15 Pain Relevance 0
To verify that Keap1 and SQSTM1 interact under physiological conditions, endogenous SQSTM1 was immunoprecipitated from HEK293T cells.
SQSTM1 Binding (interact) of
16) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
To confirm the association of Keap1 with SQSTM1, we expressed human Keap1-V5/His or SQSTM1-FLAG/His in HEK293T or HeLa cells and subjected cell lysates to anti-V5 or anti-FLAG pulldown followed by immunoblotting for Keap1 or SQSTM1.
SQSTM1 Binding (immunoblotting) of in HeLa
17) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
As well as confirming the identity of some Keap1-interacting proteins already documented in the literature, this approach has identified the multifunctional protein sequestosome 1 (SQSTM1, also known as p62) (18) as a physiological binding partner of Keap1.
SQSTM1 Binding (binding) of
18) Confidence 0.33 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.08 Pain Relevance 0
Previous studies have shown that PWC170, a measure of cardiorespiratory fitness, is associated with obesity [29] and bone mineral density at the femoral neck and spine [30] in girls, but the role of PWC170 in the pathogenesis of knee OA is novel.
PWC170 Binding (associated) of in knee associated with obesity and osteoarthritis
19) Confidence 0.28 Published 2006 Journal Arthritis Res Ther Section Body Doc Link PMC1526579 Disease Relevance 0.96 Pain Relevance 0.43
Increased DNA binding for p50 and p60 was observed at 2 to 5 h after incubation while maximal activities were generally observed 8 h after stimulation for most of the ligands (Figure 3).
p60 Binding (binding) of
20) Confidence 0.07 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2768757 Disease Relevance 0 Pain Relevance 0

General Comments

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