INT192129

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Context Info
Confidence 0.67
First Reported 2006
Last Reported 2010
Negated 0
Speculated 1
Reported most in Body
Documents 24
Total Number 29
Disease Relevance 7.62
Pain Relevance 3.68

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

transport (P2rx2) plasma membrane (P2rx2)
Anatomy Link Frequency
neurons 6
keratinocytes 4
longitudinal muscle 2
ganglia 2
ileum 1
P2rx2 (Mus musculus)
Pain Link Frequency Relevance Heat
trigeminal ganglion 755 98.72 Very High Very High Very High
Lasting pain 65 98.08 Very High Very High Very High
Cholecystokinin 2 96.80 Very High Very High Very High
agonist 261 96.24 Very High Very High Very High
qutenza 335 95.60 Very High Very High Very High
Potency 30 94.52 High High
Serotonin 132 92.88 High High
antagonist 106 90.00 High High
Neurotransmitter 61 88.96 High High
Nicotine 45 88.56 High High
Disease Link Frequency Relevance Heat
Targeted Disruption 258 100.00 Very High Very High Very High
Ganglion Cysts 1300 99.12 Very High Very High Very High
Pain 331 98.08 Very High Very High Very High
Neurological Disease 10 81.92 Quite High
Nociception 105 77.44 Quite High
Headache Disorders 15 72.64 Quite High
Cv General 4 Under Development 2 53.28 Quite High
Apoptosis 22 52.80 Quite High
Migraine With Aura 25 50.00 Quite Low
Migraine Disorders 65 49.40 Quite Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
These data indicate that KO of P2X2 or P2X3 subunits does not impair propulsive motility of the mouse colon in vivo.


Gene_expression (subunits) of P2X2 in colon associated with targeted disruption
1) Confidence 0.67 Published 2010 Journal Frontiers in Neuroscience Section Body Doc Link PMC2858605 Disease Relevance 0.82 Pain Relevance 0
This result indicates that P2X2 and P2X3 gene deletion does not compromise overall longitudinal muscle function.
Gene_expression (deletion) of P2X2 in longitudinal muscle
2) Confidence 0.67 Published 2010 Journal Frontiers in Neuroscience Section Body Doc Link PMC2858605 Disease Relevance 0.39 Pain Relevance 0.11
WT, P2X2 and P2X3 KO mice were injected with 5-hydroxytryptophan (100?
Gene_expression (injected) of P2X2 associated with targeted disruption
3) Confidence 0.58 Published 2010 Journal Frontiers in Neuroscience Section Body Doc Link PMC2858605 Disease Relevance 0.10 Pain Relevance 0
Previous studies have shown that small intestinal peristalsis studied in vitro is impaired tissues from P2X2 and P2X3 KO mice (Bian et al., 2003; Ren et al., 2003).
Gene_expression (tissues) of P2X2 associated with targeted disruption
4) Confidence 0.58 Published 2010 Journal Frontiers in Neuroscience Section Body Doc Link PMC2858605 Disease Relevance 0.31 Pain Relevance 0
Alternatively, P2X2 receptors may normally mediate ATP-induced contractions of the longitudinal muscle but that there are compensatory changes in purinergic receptor expression in P2X2 KO mice.
Gene_expression (expression) of P2X2 in longitudinal muscle associated with targeted disruption
5) Confidence 0.58 Published 2010 Journal Frontiers in Neuroscience Section Body Doc Link PMC2858605 Disease Relevance 0.22 Pain Relevance 0
5) could cause activation of P2X2 channels expressed in HEK293 cells in the system (Fig. 3a, b) although time to activation of P2X2-mediated currents varied because of the stopped flow and the fact that it requires 1–2 min for 4?
Gene_expression (nnels exp) of P2X2
6) Confidence 0.58 Published 2009 Journal Pflugers Arch Section Body Doc Link PMC2745623 Disease Relevance 0 Pain Relevance 0.08
Similar P2X2 receptor-mediated currents in the HEK293 cells were observed in the co-culture with keratinocytes from TRPV1KO mice (Fig. 2c, n?
Gene_expression (receptor) of P2X2 in keratinocytes
7) Confidence 0.51 Published 2009 Journal Pflugers Arch Section Body Doc Link PMC2745623 Disease Relevance 0 Pain Relevance 0
Functional expression of P2X2 receptors in the HEK293 cell was confirmed by ATP (100 µM) application in the condition with two cell types apart to avoid possible effect of ATP-induced ATP release from keratinocytes (Fig. 2b).
Gene_expression (expression) of P2X2 in keratinocytes
8) Confidence 0.51 Published 2009 Journal Pflugers Arch Section Body Doc Link PMC2745623 Disease Relevance 0.05 Pain Relevance 0.03
40°C) to the co-culture of wild-type keratinocytes with HEK293 cells expressing P2X2 receptors, P2X2-like inwardly rectifying currents were recorded from the whole-cell patch-clamped HEK293 cells only when placed in close proximity to the keratinocytes (less than 10 µm in the distance; Fig. 2b, n?
Gene_expression (expressing) of P2X2 in keratinocytes
9) Confidence 0.51 Published 2009 Journal Pflugers Arch Section Body Doc Link PMC2745623 Disease Relevance 0.07 Pain Relevance 0.03
Likewise, no change in P2X2 subunit expression was detected.
Gene_expression (expression) of P2X2
10) Confidence 0.42 Published 2010 Journal Mol Pain Section Body Doc Link PMC2940876 Disease Relevance 0.31 Pain Relevance 0.50
For immunofluorescence microscopy, the following antibodies were used: anti-P2X3, anti-P2X2, anti-TRPV1 and anti-CaV2.1 ?
Gene_expression (anti) of P2X2
11) Confidence 0.42 Published 2010 Journal Mol Pain Section Body Doc Link PMC2940876 Disease Relevance 0.13 Pain Relevance 0
For immunofluorescence microscopy, the following antibodies were used: anti-P2X3, anti-P2X2, anti-TRPV1 and anti-CaV2.1 ?
Gene_expression (anti) of P2X2
12) Confidence 0.42 Published 2010 Journal Mol Pain Section Body Doc Link PMC2940876 Disease Relevance 0.13 Pain Relevance 0
For immunofluorescence microscopy, the following antibodies were used: anti-P2X3, anti-P2X2, anti-TRPV1 and anti-CaV2.1 ?
Gene_expression (anti) of P2X2
13) Confidence 0.42 Published 2010 Journal Mol Pain Section Body Doc Link PMC2940876 Disease Relevance 0.13 Pain Relevance 0
Mouse trigeminal neurons usually express a low level of heteromeric P2X2/3 receptors mediating a sustained inward current following the initial transient peak [22].
Gene_expression (express) of P2X2 in neurons
14) Confidence 0.42 Published 2010 Journal Mol Pain Section Body Doc Link PMC2940876 Disease Relevance 0 Pain Relevance 0.10
Fig. 2P2X2-mediated currents were observed upon heating in HEK293 cells expressing P2X2 co-cultured with mouse keratinocytes. a Cartoons showing a bio-sensor system.
Gene_expression (expressing) of P2X2 in keratinocytes
15) Confidence 0.39 Published 2009 Journal Pflugers Arch Section Body Doc Link PMC2745623 Disease Relevance 0 Pain Relevance 0.08
Western immunoblotting experiments were performed to investigate protein expression levels for P2X3, P2X2 and TRPV1 receptors in rat and mouse ganglia as well in culture (Fig. 2B).
Spec (investigate) Gene_expression (expression) of P2X2 in ganglia
16) Confidence 0.39 Published 2006 Journal Mol Pain Section Body Doc Link PMC1448213 Disease Relevance 0.19 Pain Relevance 0
In culture, P2X2 was expressed predominantly in medium (50%) while TRPV1 in small size neurons (65–70%).
Gene_expression (expressed) of P2X2 in neurons
17) Confidence 0.34 Published 2006 Journal Mol Pain Section Body Doc Link PMC1448213 Disease Relevance 0.48 Pain Relevance 0.07
Because P2X2 and TRPV1 receptors are also expressed in non-neuronal cells, while the P2X3 receptor is not [16,17], it was important to assess the contribution of P2X2, P2X3 and TRPV1 mRNAs in 3 week-old cultures, containing no neurons (?
Gene_expression (expressed) of P2X2 in neurons
18) Confidence 0.34 Published 2006 Journal Mol Pain Section Body Doc Link PMC1448213 Disease Relevance 0.37 Pain Relevance 0.03
Although the global number of P2X2 receptor expressing neurons was always comparatively low (see Fig. 3 and Table 1), once in culture, the percentage of P2X2-positive neurons expressing also P2X3 receptors largely rose.
Gene_expression (expressing) of P2X2 in neurons
19) Confidence 0.34 Published 2006 Journal Mol Pain Section Body Doc Link PMC1448213 Disease Relevance 0.48 Pain Relevance 0.26
While in the case of the TRPV1 receptor there was a significant increment already at 24 h in culture (1.5 fold, P < 0.05), this was not paralleled by P2X2 and P2X3 expression that showed no substantial change in culture with respect to ganglion (Fig. 2C).
Gene_expression (expression) of P2X2 in ganglion associated with ganglion cysts
20) Confidence 0.34 Published 2006 Journal Mol Pain Section Body Doc Link PMC1448213 Disease Relevance 0.27 Pain Relevance 0

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