INT192957

Context Info Confidence 0.69 First Reported 2006 Last Reported 2008 Negated 0 Speculated 0 Reported most in Body Documents 5 Total Number 5 Disease Relevance 2.19 Pain Relevance 0.23

signal transduction (KIT) extracellular space (KIT) plasma membrane (KIT) nucleus (KIT) cytoplasm (KIT)

KIT (Homo sapiens)

Pain Link Frequency Relevance Heat sodium channel 3 89.88 cytokine 16 84.08 cva 50 81.76 Hippocampus 11 77.84 chemokine 20 50.00 Neuronal excitability 1 50.00 imagery 2 24.48 Pain 17 5.00 Action potential 17 5.00 Inflammation 15 5.00

Disease Link Frequency Relevance Heat Seminoma 1 99.04 Malignant Fibrous Histiocytoma 1 98.68 Germ Cell And Embryonal Neoplasms 1 98.00 Cancer 90 96.24 Thyroid Neoplasm 3 95.84 Piebaldism 1 91.56 Disease 16 90.88 Apoptosis 27 87.24 Hemangiosarcoma 2 83.12 Cv General 4 Under Development 41 81.76

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

For fluorescence labeling of target cDNAs, 20 µg of total RNA from universal human reference (Strategene, USA) and experiment samples (RNA extracted from infected cells using Qiagen RNeasy Mini Kit ) were reverse transcribed in the presence of Cy3-dUTP and Cy5-dUTP (Amersham Biosciences, UK) using the Superscript reverse transcription kit (Invitrogen, USA).

Transcription (transcribed) of Kit

1) Confidence 0.69 Published 2007 Journal PLoS Neglected Tropical Diseases Section Body Doc Link PMC2100376 Disease Relevance 0.05 Pain Relevance 0

Immediately after isolation, RNA was transcribed into cDNA using an iScript cDNA kit (Bio-Rad).

Transcription (transcribed) of kit

2) Confidence 0.69 Published 2008 Journal Mol Pain Section Body Doc Link PMC2562993 Disease Relevance 0 Pain Relevance 0.04

Although an over-expression of CD117 mRNA was noted in cell lines of malignant fibrous histiocytomas [36], IHC evaluation of such tumors for c-kit revealed only one positive reaction out of 43 cases [51].

Transcription (expression) of CD117 associated with malignant fibrous histiocytoma and cancer

3) Confidence 0.53 Published 2006 Journal Diagn Pathol Section Body Doc Link PMC1475889 Disease Relevance 1.37 Pain Relevance 0

The upregulation of these miRNAs was strongest associated with a dramatic loss of Kit transcript and its protein.

Transcription (transcript) of Kit

4) Confidence 0.29 Published 2007 Journal Mol Cancer Section Body Doc Link PMC2098778 Disease Relevance 0.57 Pain Relevance 0

In addition to growth factors, the RT-PCR study has demonstrated that both primary human MSCs and B10 cells express mRNA for VEGFR, CXCR4 and c-kit, cellular receptors known for close involvement in cellular migration (Figure 10).

Transcription (express) of c-kit

5) Confidence 0.22 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2092394 Disease Relevance 0.20 Pain Relevance 0.18

General Comments

This test has worked.