INT193428

Context Info Confidence 0.75 First Reported 2006 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 20 Total Number 22 Disease Relevance 8.15 Pain Relevance 1.13

molecular_function (Muc5b) cellular_component (Muc5b) biological_process (Muc5b)

Anatomy Link Frequency lung 7 epithelium 3 glands 2 ciliary 1 Muc5b (Mus musculus)

Pain Link Frequency Relevance Heat cytokine 66 99.92 Inflammation 297 99.72 fibrosis 210 92.68 Inflammatory marker 6 86.92 agonist 21 81.20 addiction 3 45.68 corticosteroid 42 43.04 chemokine 3 39.64 Inflammatory mediators 45 34.84 Inflammatory response 15 8.88

Disease Link Frequency Relevance Heat Adhesions 21 100.00 INFLAMMATION 336 99.72 Disease 183 98.70 Injury 27 98.28 Respiratory Syncytial Virus 216 96.76 Asthma 132 96.40 Infection 375 95.80 Cystic Fibrosis 210 92.68 Bacterial Infection 15 92.68 Pulmonary Disease 93 92.24

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Event

Anatomy

Negation

Speculation

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Disease term

The expression level of Muc4 in lung of healthy mice was higher than the expression levels of both Muc5b and Muc5ac RNAs.

Gene_expression (expression) of Muc5b in lung

1) Confidence 0.75 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0 Pain Relevance 0

At days 1 and 4, the peak of expression of the large secreted gel-forming mucin Muc5b was higher for the control group diet in comparison with the n-3 PUFAS treated group suggesting a suppressive effect of n-3 PUFAs on inoculated mice.

Gene_expression (expression) of Muc5b

2) Confidence 0.75 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.17 Pain Relevance 0

In parallel with the bacterial clearance by ciliary transport, expression of Muc5b decreased almost to baseline levels on day 7.

Gene_expression (expression) of Muc5b in ciliary

3) Confidence 0.75 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.30 Pain Relevance 0.03

Muc5b expression increased significantly for the n-3 PUFAs treated group between days 1 and 4.

Gene_expression (expression) of Muc5b

4) Confidence 0.75 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.16 Pain Relevance 0

In our mouse model, we followed Muc5b, Muc5ac and Muc4 while the Muc2 messenger was barely undetectable in lung tissue and did not show any variation prior or after instillation by sterile or PAO1-laden agarose beads (data not shown).

Gene_expression (followed) of Muc5b in lung

5) Confidence 0.65 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.19 Pain Relevance 0

For each day, the diet has no impact on the two mucins gene expression except at day 7 on the Muc5b transcripts where the level of expression is n-3 < n-6 (p < 0.05).

Gene_expression (expression) of Muc5b

6) Confidence 0.65 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0 Pain Relevance 0

As it is well-known, rodent models only partially resemble the native architecture and abundance of submucosal glands in the human airways [39], it is therefore not surprising that Muc5b is almost undetectable using qPCR in the lungs of healthy mice.

Gene_expression (undetectable) of Muc5b in glands

7) Confidence 0.65 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.17 Pain Relevance 0

The expression level of Muc4 in lung of healthy mice was higher than the expression levels of both Muc5b and Muc5ac RNAs.

Gene_expression (expression) of Muc5b in lung

8) Confidence 0.65 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0 Pain Relevance 0

PAO1 lung inoculation induced two opposite expression patterns of Muc5b and Muc4: Muc5b is highly upregulated with a peak at day 4 showing a suppressive effect of n-3 PUFAs on the Muc5b response to the infection while Muc4 expression decreased to the lowest level at days 1 or 4 for sterile beads and at day 7 for Pseudomonas-laden agarose beads.

Gene_expression (expression) of Muc5b in lung associated with infection

9) Confidence 0.65 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.24 Pain Relevance 0

MUC5AC and MUC2 are expressed in the goblet cells of the superficial airway epithelium, whereas MUC5B is expressed in the mucosal cells of the submucosal glands [9].

Gene_expression (expressed) of MUC5B in glands

10) Confidence 0.59 Published 2010 Journal Respir Res Section Body Doc Link PMC3014901 Disease Relevance 0.69 Pain Relevance 0.04

The other one is made of the five, large secreted gel-forming mucins MUC6, MUC2, MUC5AC, MUC5B and MUC19, which are conserved between human and mouse [13-15] and form oligomeric structures [9,16,17].

Gene_expression (made) of MUC5B

11) Confidence 0.58 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.51 Pain Relevance 0.16

At the cellular level, MUC5AC is expressed in the goblet cells of main bronchi and bronchioles and MUC5B is expressed in bronchiolar epithelium and in submucosal gland epithelium while MUC4 transcripts are detected in the epithelium of main bronchi and bronchioles [19-21].

Gene_expression (expressed) of MUC5B in epithelium

12) Confidence 0.58 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.30 Pain Relevance 0.03

MUC5AC, MUC5B and MUC4, which are conserved mucins between human and rodent [30,31], are three predominant large mucins expressed in the lung.

Gene_expression (expressed) of MUC5B in lung

13) Confidence 0.58 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.34 Pain Relevance 0.05

Many studies have focused on MUC5AC and MUC2 regulation in airway diseases using mainly airway cell and explant cultures [32-34] while the expression of the oligomeric mucin MUC5B and the membrane-bound mucin MUC4 have not yet been investigated.

Gene_expression (expression) of MUC5B associated with disease

14) Confidence 0.58 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.40 Pain Relevance 0.07

In human, it has been reported that MUC5AC expression, measured using real-time RT-PCR, is much higher than both MUC5B and MUC4 in homogenates of endobronchial biopsies from healthy subjects or subjects with asthma [40].

Gene_expression (expression) of MUC5B associated with asthma

15) Confidence 0.58 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.18 Pain Relevance 0

Among the identified mucins, the large anchored-mucin MUC4 and the two large gel-forming mucins MUC5B and MUC5AC seem highly expressed in normal and/or abnormal human lung while MUC2 is almost not detectable [18].

Gene_expression (expressed) of MUC5B in lung

16) Confidence 0.51 Published 2007 Journal Respir Res Section Body Doc Link PMC1899493 Disease Relevance 0.30 Pain Relevance 0.06

Western Blotting showed the presence of MUC2, MUC5AC and MUC5B in the sample (Fig. 5).

Gene_expression (presence) of MUC5B

17) Confidence 0.48 Published 2007 Journal International Journal of Medical Sciences Section Body Doc Link PMC1852400 Disease Relevance 0.05 Pain Relevance 0

We observed no differences in MUC5B, MUC2, or MUC6 mRNA expression between mice in the control and the smoke injury groups (data not shown).

Gene_expression (expression) of MUC5B associated with injury

18) Confidence 0.46 Published 2010 Journal Respir Res Section Body Doc Link PMC3014901 Disease Relevance 0.50 Pain Relevance 0.04

Smoke exposure caused an increase in the production of mucus in the airway epithelium of the mice along with an increase in MUC5AC gene and protein expression, while the expression of MUC5B was not increased compared with control.

Gene_expression (expression) of MUC5B in epithelium

19) Confidence 0.46 Published 2010 Journal Respir Res Section Abstract Doc Link PMC3014901 Disease Relevance 0.62 Pain Relevance 0

The expression of MUC-5A, which is a marker for mucus production, was also decreased by combined FPS treatment, but not significantly by either FP or Sal alone.

Gene_expression (expression) of MUC-5A

20) Confidence 0.29 Published 2006 Journal Virol J Section Body Doc Link PMC1488829 Disease Relevance 0.88 Pain Relevance 0.13

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