INT195946

Context Info Confidence 0.75 First Reported 2006 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 9 Total Number 10 Disease Relevance 1.03 Pain Relevance 0.89

cytosol (Hprt1) transferase activity, transferring glycosyl groups (Hprt1) cytoplasm (Hprt1)

Hprt1 (Rattus norvegicus)

Pain Link Frequency Relevance Heat Spinal cord 36 91.28 Neuropeptide 11 88.80 Nav1.7 3 76.88 nociceptor 9 76.72 Nav1.1 3 76.52 Glutamate receptor 12 69.24 Nav1.9 9 63.04 sodium channel 9 62.44 Morphine 226 56.52 Inflammation 111 50.00

Disease Link Frequency Relevance Heat Sprains And Strains 153 98.20 Nervous System Malformation 6 96.96 Apoptosis 57 95.00 Myocardial Infarction 43 93.24 Stress 13 57.76 Coronary Heart Disease 4 54.96 Urological Neuroanatomy 5 50.88 INFLAMMATION 134 50.00 Injury 73 16.88 Disease 6 14.96

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

One finding that was unexpected was the significant difference in expression level of the reference genes used on the array plate (Ldha, Rplp1, Hprt, and Rpl13a).

Gene_expression (expression) of Hprt

1) Confidence 0.75 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC3001741 Disease Relevance 0.29 Pain Relevance 0.16

Copy number for each molecule (all P2X subunits and Lyn tyrosine kinase) was determined based on comparisons to standard curves, and for each sample, the copy number for each molecule was normalized to the expression of the endogenous housekeeping gene hypoxanthine phosphoribosyl transferase (HPRT).

Gene_expression (expression) of hypoxanthine phosphoribosyl transferase

2) Confidence 0.75 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0

Primer pairs for mouse Hprt and pro-IL-1?

Gene_expression (pro) of Hprt

3) Confidence 0.65 Published 2010 Journal Mol Pain Section Body Doc Link PMC2959021 Disease Relevance 0.05 Pain Relevance 0

Expression of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) transcript with stable level between four strains was quantified to control for variation in cDNA amounts.

Gene_expression (Expression) of hypoxanthine guanine phosphoribosyl transferase 1 associated with sprains and strains

4) Confidence 0.65 Published 2006 Journal BMC Genomics Section Body Doc Link PMC1553451 Disease Relevance 0.10 Pain Relevance 0.03

Expression of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) transcript with a stable level between the strains and after the treatment was quantified to control for variation in cDNA amounts.

Gene_expression (Expression) of Hprt1 associated with sprains and strains

5) Confidence 0.58 Published 2007 Journal Genome Biol Section Body Doc Link PMC2394777 Disease Relevance 0.10 Pain Relevance 0.06

The significant difference in expression of the reference genes (Rplp1, Ldha, Hprt, and Rpl13a) is also intriguing.

Gene_expression (expression) of Hprt

6) Confidence 0.58 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC3001741 Disease Relevance 0.06 Pain Relevance 0.05

The candidate genes Arb, Rpl32, Rpl4, Tbp, Polr2a, Hprt1, Pgk1, Ppia for each species and 18S ribosomal RNA were quantified by RT-qPCR.

Gene_expression (quantified) of Hprt1

7) Confidence 0.50 Published 2010 Journal BMC Mol Biol Section Body Doc Link PMC2907514 Disease Relevance 0.44 Pain Relevance 0

Of the nine Na channels, 3 showed much higher expression relative to HPRT than the others: Nav 1.1, 1.7, and 1.8 (Figure 6A).

Gene_expression (expression) of HPRT

8) Confidence 0.28 Published 2008 Journal Mol Pain Section Body Doc Link PMC2562993 Disease Relevance 0 Pain Relevance 0.28

Expression data were normalized to that of the housekeeping gene HPRT.

Gene_expression (Expression) of HPRT

9) Confidence 0.25 Published 2008 Journal Mol Pain Section Body Doc Link PMC2562993 Disease Relevance 0 Pain Relevance 0.28

Fluorescence was normalized with the reference dye ROX, and gene expression was normalized to the housekeeping gene hypoxanthine ribosyltransferase (HPRT; [25]), run on the same plate.

Gene_expression (ribosyltransferase) of HPRT

10) Confidence 0.25 Published 2008 Journal Mol Pain Section Body Doc Link PMC2562993 Disease Relevance 0 Pain Relevance 0.03

General Comments

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