INT195946
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
One finding that was unexpected was the significant difference in expression level of the reference genes used on the array plate (Ldha, Rplp1, Hprt, and Rpl13a). | |||||||||||||||
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Copy number for each molecule (all P2X subunits and Lyn tyrosine kinase) was determined based on comparisons to standard curves, and for each sample, the copy number for each molecule was normalized to the expression of the endogenous housekeeping gene hypoxanthine phosphoribosyl transferase (HPRT).
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Primer pairs for mouse Hprt and pro-IL-1? | |||||||||||||||
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Expression of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) transcript with stable level between four strains was quantified to control for variation in cDNA amounts. | |||||||||||||||
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Expression of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) transcript with a stable level between the strains and after the treatment was quantified to control for variation in cDNA amounts. | |||||||||||||||
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The significant difference in expression of the reference genes (Rplp1, Ldha, Hprt, and Rpl13a) is also intriguing. | |||||||||||||||
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The candidate genes Arb, Rpl32, Rpl4, Tbp, Polr2a, Hprt1, Pgk1, Ppia for each species and 18S ribosomal RNA were quantified by RT-qPCR. | |||||||||||||||
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Of the nine Na channels, 3 showed much higher expression relative to HPRT than the others: Nav 1.1, 1.7, and 1.8 (Figure 6A). | |||||||||||||||
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Expression data were normalized to that of the housekeeping gene HPRT. | |||||||||||||||
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Fluorescence was normalized with the reference dye ROX, and gene expression was normalized to the housekeeping gene hypoxanthine ribosyltransferase (HPRT; [25]), run on the same plate. | |||||||||||||||
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