INT195976

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Context Info
Confidence 0.75
First Reported 2006
Last Reported 2010
Negated 0
Speculated 1
Reported most in Body
Documents 26
Total Number 28
Disease Relevance 14.23
Pain Relevance 0.60

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

oxidoreductase activity (Aldh1a1) cytoplasm (Aldh1a1)
Anatomy Link Frequency
CPA 12
stroma 1
peripheral nervous system 1
Aldh1a1 (Mus musculus)
Pain Link Frequency Relevance Heat
Peripheral nervous system 1 98.84 Very High Very High Very High
alcohol 6 97.76 Very High Very High Very High
Spinal cord 2 88.76 High High
addiction 3 75.04 Quite High
opiate 3 74.20 Quite High
Opioid 23 71.20 Quite High
Morphine 54 54.48 Quite High
antagonist 7 52.64 Quite High
anesthesia 48 43.32 Quite Low
Ventral tegmentum 5 39.76 Quite Low
Disease Link Frequency Relevance Heat
Cancer 3484 100.00 Very High Very High Very High
Colorectal Cancer 640 100.00 Very High Very High Very High
Targeted Disruption 29 99.82 Very High Very High Very High
Infection 57 99.04 Very High Very High Very High
Residual Neoplasm 202 99.00 Very High Very High Very High
Sprains And Strains 98 98.38 Very High Very High Very High
Death 63 98.08 Very High Very High Very High
Rheumatoid Arthritis 81 91.48 High High
Glioma 30 90.88 High High
Hematological Disease 20 90.80 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Taqman™ qRT-PCR studies of UM-C6 tumor cells verified that ALDH1A1 gene expression was reduced 88% versus Luciferase-targeted shRNA transduced control cells, whereas neither ALDH3A1 nor ALDH5A1 gene expression were altered (Figure 8A).
Gene_expression (expression) of ALDH1A1 associated with cancer
1) Confidence 0.75 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.66 Pain Relevance 0
ALDH1A1 gene expression is 2.8-fold higher in CoCSC than in NTG cells, and despite slight elevation of ALDH3A1 expression in CoCSC versus NTG populations (Figure 3C), its message is barely detectible in either tumor line.
Gene_expression (expression) of ALDH1A1 associated with cancer
2) Confidence 0.75 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.82 Pain Relevance 0
UM-C6 tumors were chosen because of their high ALDH1A1 expression and relatively low expression of other ALDH family members (e.g.
Gene_expression (expression) of ALDH1A1 associated with cancer
3) Confidence 0.75 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.52 Pain Relevance 0
Although ALDH1A1, ALDH3A1, and ALDH5A1 gene products can degrade biologically active CPA metabolites [20], [21], DEAB appears to specifically inhibit ALDH1 [34].
Gene_expression (products) of ALDH1A1 in CPA
4) Confidence 0.75 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.47 Pain Relevance 0
Seeing that ESA+CD44+ tumor cells have high ALDH activity, the frequency of ESA+CD44+ALDH+ cells is increased in tumors from mice treated with CPA, and ALDH1A1 gene expression is elevated in CPA-resistant CoCSC, we therefore sought to determine whether resistance to CPA was mediated by ALDH1 enzyme activity.
Gene_expression (expression) of ALDH1A1 in CPA associated with cancer
5) Confidence 0.75 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.83 Pain Relevance 0
Intracellular levels of ALDH1 and nuclear c-Myc, for example, were more prevalent in CPA-treated tumors (Figure 4C).
Gene_expression (levels) of ALDH1 in CPA associated with cancer
6) Confidence 0.75 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.79 Pain Relevance 0
In Taqman™ qRT-PCR analysis of UM-C4 and UM-C6 tumors, ALDH1A1 is generally expressed at higher levels than ALDH3A1 and ALDH5A1.
Gene_expression (expressed) of ALDH1A1 associated with cancer
7) Confidence 0.75 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.87 Pain Relevance 0
Furthermore, striatal gene expression of quasi-congenic strains and their progenitors C57BL/6By and BALB/cJ were previously described and basal expression of aldehyde dehydrogenase genes (Aldh1a1 and Aldh7a1) was suggested as the factor implicated in alcohol preference [26].
Gene_expression (expression) of Aldh1a1 associated with sprains and strains and alcohol
8) Confidence 0.72 Published 2006 Journal BMC Genomics Section Body Doc Link PMC1553451 Disease Relevance 0.59 Pain Relevance 0.40
For Immunofluorescence staining of ALDH1 (Abcam), frozen sections were fixed with ?
Gene_expression (staining) of ALDH1
9) Confidence 0.65 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.15 Pain Relevance 0
We also reveal that inherent resistance mechanisms differentially expressed within tumor subpopulations, such as ALDH1 enzymatic activity in CoCSC, can explain the inability of chemotherapeutic agents to improve overall survival despite tumor regression.
Gene_expression (expressed) of ALDH1 associated with cancer
10) Confidence 0.65 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.85 Pain Relevance 0
Since ALDH1A1 gene expression is elevated in TG versus NTG cells, ALDH1 enzymatic activity is disproportionably high in cells with the CoCSC phenotype, and this activity may mediate resistance to CPA, we next asked whether the frequency of ESA+CD44+ALDH+ cells was elevated in UM-C4, UM-C6 and OMP-C8 tumors from mice receiving CPA versus those being administered vehicle.
Gene_expression (expression) of ALDH1A1 in CPA associated with cancer
11) Confidence 0.65 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.37 Pain Relevance 0
To assess clonality using lentiviral insertion site analysis, or introduce shRNA targeting ALDH1A1 or Luciferase, UM-C4 and UM-C6 tumor cells were transduced in vitro over a 3–4 day period.
Gene_expression (targeting) of ALDH1A1 associated with cancer
12) Confidence 0.65 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.17 Pain Relevance 0
Consistent with the hypothesis that ALDH1 may have a role in mediating resistance to CPA, ALDH1A1 gene expression was further elevated in residual cells with the CoCSC phenotype, but ALDH3A1 and ALDH5A1 were not (Figures 4B & S3).
Gene_expression (expression) of ALDH1A1 in CPA
13) Confidence 0.65 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.57 Pain Relevance 0
Interestingly, ALDH1 staining in CPA-treated tumors appeared to localize to the apical surface, where it may be hypothesized to intercept CPA metabolites as they enter the cell.
Gene_expression (staining) of ALDH1 in CPA associated with cancer
14) Confidence 0.65 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.75 Pain Relevance 0
These observations are supported by qRT-PCR using TG or NTG cells isolated by FACS, which show that ALDH1A1 is the predominant cytoplasmic ALDH enzyme in colorectal tumors and its expression is further increased in residual tumor cells following therapy with CPA; consistent with the phenotypic increase in ALDH+ cells among the CoCSC phenotype.
Gene_expression (expression) of ALDH1A1 in CPA associated with colorectal cancer and residual neoplasm
15) Confidence 0.65 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.83 Pain Relevance 0
The preferential expression of ALDH1A1 in TG cells supports observed enzymatic activity measurements among the human ESA+CD44+ subpopulation, wherein the majority of these cells have high DEAB-sensitive, ALDH1 activity.
Gene_expression (expression) of ALDH1A1
16) Confidence 0.65 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.60 Pain Relevance 0
ALDH1A1, MYC, and MYB are enriched in residual TG cells post CPA therapy
Gene_expression (enriched) of ALDH1A1 in CPA
17) Confidence 0.65 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.36 Pain Relevance 0
Preferential ALDH1A1 gene expression in CoCSC and sensitization of colorectal tumor cells to 4-HC by inhibiting ALDH1, together strongly suggest that ALDH1 enzyme activity mediates CPA resistance.
Gene_expression ( gene expr) of ALDH1A1 in CPA associated with colorectal cancer
18) Confidence 0.58 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.56 Pain Relevance 0
In agreement with results observed in vitro with the ALDH1 inhibitor, DEAB, shRNA targeting of ALDH1A1 gene expression appeared to sensitize tumors to CPA in vivo, as tumor growth essentially stopped, whereas ALDH1A1-targeted shRNA cells treated with vehicle continued to grow (Figure 8B; black triangles).
Gene_expression (expression) of ALDH1A1 in CPA associated with cancer
19) Confidence 0.58 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.93 Pain Relevance 0
The above in vitro studies strongly suggest that the ALDH1A1 gene product, ALDH1, mediates resistance to CPA.
Gene_expression (product) of ALDH1 in CPA
20) Confidence 0.58 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2413402 Disease Relevance 0.42 Pain Relevance 0

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