INT199096

Context Info Confidence 0.42 First Reported 2006 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 8 Total Number 9 Disease Relevance 1.55 Pain Relevance 0.53

cell proliferation (Ncf1) cytosol (Ncf1) Golgi apparatus (Ncf1) plasma membrane (Ncf1) cytoplasm (Ncf1)

Anatomy Link Frequency plasma 1 be cell 1 Ncf1 (Mus musculus)

Pain Link Frequency Relevance Heat Kinase C 6 96.92 Inflammatory stimuli 12 94.76 Enkephalin 1 78.20 Inflammation 119 71.76 Inflammatory response 46 68.00 Serotonin 6 67.00 cva 4 45.44 ischemia 14 39.56 Inflammatory mediators 15 24.56 cINOD 36 5.00

Disease Link Frequency Relevance Heat Hemorrhagic Shock 64 97.52 INFLAMMATION 184 94.44 Adhesions 10 90.80 Disease 367 74.96 Injury 48 64.28 Drug Induced Neurotoxicity 52 62.68 Stress 27 59.96 Targeted Disruption 6 51.20 Hemorrhage 4 45.44 Lung Injury 2 43.96

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As the activation of a functional PHOX enzyme requires the phorsphorylation of p47phox followed by the translocation of the p47phox, p67phox, and p40phox complex to the plasma membrane, our results suggest that SN works on one of the earliest stages of PHOX activation to inhibit the assembly of the PHOX enzyme complex.

Phosphorylation (phorsphorylation) of p47phox in plasma

1) Confidence 0.42 Published 2007 Journal J Neuroinflammation Section Body Doc Link PMC2064906 Disease Relevance 0.38 Pain Relevance 0.18

It has been demonstrated that HS through HMGB1 activates the TLR4-MyD88-IRAK4 signaling pathway and further activates p38 MAPK and Akt pathways to initiate phosphorylation of p47phox and subsequent activation of NADPH oxidase (Figure 3) [2].

Phosphorylation (phosphorylation) of p47phox associated with hemorrhagic shock

2) Confidence 0.24 Published 2010 Journal Mediators of Inflammation Section Body Doc Link PMC2913855 Disease Relevance 0.24 Pain Relevance 0

Analysis of p47phox and p67phox phosphorylation has been investigated with the use of specific protein kinase inhibitors revealing that protein kinase C (PKC) [37-39], p21-activated kinase-1 (PAK1) [40], mitogen-activated protein kinase (MAPK) [41], Akt [42], and phosphatidylinositol-3 kinase (PI3K) [43] phophorylate p47phox or p67phox.

Phosphorylation (phosphorylation) of p47phox associated with kinase c

3) Confidence 0.22 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1637099 Disease Relevance 0 Pain Relevance 0.05

These cells also exhibit defects in the phosphorylation and translocation of p47phox [105] and p67phox [106] to the membrane.

Phosphorylation (phosphorylation) of p47phox

4) Confidence 0.22 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1637099 Disease Relevance 0.39 Pain Relevance 0.06

The diversity of kinases implicated in the phosphorylation of p47phox and p67phox suggests that the intracellular signaling pathways responsible for this phosphorylation event are complex and may be cell type and stimulus specific.

Phosphorylation (phosphorylation) of p47phox in be cell

5) Confidence 0.22 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1637099 Disease Relevance 0 Pain Relevance 0.05

These cytoplasmic signaling events stimulate the phosphorylation of p47phox and p67phox and the GDP/GTP exchange on Rac.

Phosphorylation (phosphorylation) of p47phox

6) Confidence 0.22 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1637099 Disease Relevance 0.17 Pain Relevance 0.08

Analysis of p47phox and p67phox phosphorylation has been investigated with the use of specific protein kinase inhibitors revealing that protein kinase C (PKC) [37-39], p21-activated kinase-1 (PAK1) [40], mitogen-activated protein kinase (MAPK) [41], Akt [42], and phosphatidylinositol-3 kinase (PI3K) [43] phophorylate p47phox or p67phox.

Phosphorylation (phophorylate) of p47phox associated with kinase c

7) Confidence 0.22 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1637099 Disease Relevance 0 Pain Relevance 0.05

Upon stimulation, the cytosolic component p47phox is phosphorylated and the entire cytosolic complex migrates to the membrane where it associates with cytochrome b558 to assemble the active oxidase [58].

Phosphorylation (phosphorylated) of p47phox

8) Confidence 0.21 Published 2010 Journal Mediators of Inflammation Section Body Doc Link PMC2913855 Disease Relevance 0.31 Pain Relevance 0

Cytosolic p47phox and p67phox are often found as a complex and both require phosphorylation prior to their translocation to the membrane [34,36].

Phosphorylation (phosphorylation) of p47phox

9) Confidence 0.08 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1637099 Disease Relevance 0.05 Pain Relevance 0.07

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