INT199255

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Context Info
Confidence 0.41
First Reported 2006
Last Reported 2008
Negated 0
Speculated 2
Reported most in Body
Documents 14
Total Number 16
Disease Relevance 1.43
Pain Relevance 2.94

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytosol (Rest) nucleus (Rest) intracellular (Rest)
DNA binding (Rest) protein complex (Rest) transcription factor binding (Rest)
Anatomy Link Frequency
neuron 3
NS20Y 2
proximal 1
nucleus 1
upper 1
Rest (Mus musculus)
Pain Link Frequency Relevance Heat
mu opioid receptor 1094 99.64 Very High Very High Very High
opioid receptor 43 76.72 Quite High
Central nervous system 39 73.68 Quite High
agonist 2 68.48 Quite High
Morphine 43 61.96 Quite High
Analgesic 15 61.04 Quite High
Oxycodone 2 21.20 Low Low
methadone 2 20.64 Low Low
Codeine 2 20.20 Low Low
Lasting pain 4 14.20 Low Low
Disease Link Frequency Relevance Heat
Repression 182 98.76 Very High Very High Very High
Small Cell Lung Cancer 4 91.92 High High
Disease 52 80.60 Quite High
Targeted Disruption 154 76.48 Quite High
Neurodegenerative Disease 13 70.64 Quite High
Cancer 5 61.76 Quite High
Necrosis 2 61.28 Quite High
Pain 5 14.20 Low Low
Hyponatremia 5 11.92 Low Low
Small Cell Lung Carcinoma 1 8.32 Low Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Taken altogether, the co-immunoprecipitation and EMSA studies demonstrate that NRSF and Sp3 factor interact with each other and physically bind to the repressive element of the MOR promoter, NRSE/GC box.


NRSF Binding (interact) of associated with mu opioid receptor
1) Confidence 0.41 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0 Pain Relevance 0.17
The self NRSP competitor competed for protein–DNA interaction efficiently (lane 3) and also mutated competitor NRmSP and NRSPm significantly competed for upper complex indicating the specific binding of NRSF and Sp3 (lanes 7 and 8).
NRSF Binding (binding) of in upper
2) Confidence 0.41 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0 Pain Relevance 0.06
The same result was obtained using c-Myc-tagged NRSF expressed in NS20Y cells implying that Sp3 binds the GC box and a direct interaction between Sp3 and NRSF is required for a synergistic repression of MOR gene expression (Figure 4B).
NRSF Binding (interaction) of in NS20Y associated with repression and mu opioid receptor
3) Confidence 0.32 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0.10 Pain Relevance 0.15
After cross-linking the proteins and DNAs with formaldehyde, cell lysates from NS20Y cells were subjected to immunoprecipitation with NRSF, HDAC1, HDAC2, Sp1, Sp3 and IRF-4 (as a negative control).
NRSF Binding (immunoprecipitation) of in NS20Y
4) Confidence 0.32 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0 Pain Relevance 0.25
The C-terminal repressor domain (CTRD) of NRSF has been shown to interact with at least one factor, the transcriptional co-repressor 2 (CoREST) that may serve as a platform protein for the recruitment of molecular machinery that imposes silencing across a chromosomal interval (14,15).
NRSF Binding (interact) of
5) Confidence 0.30 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0.13 Pain Relevance 0.17
In other words, if NRSF is abundant in the nucleus, Sp3 interacts with NRSF mainly acting as a repressor while, when NRSF is depleted in the nucleus or inactive, Sp3 might bind to the positive elements and act as a part of Sp1/Sp3 activator complex.
NRSF Spec (might) Binding (bind) of in nucleus
6) Confidence 0.30 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0.15 Pain Relevance 0.06
Although the exact mechanism of interdependency (or cooperativity) between NRSF and Sp3 for binding to NRSE/GC box remains to be elucidated, it is tempting to speculate that the function of Sp3 could be determined by the interaction with NRSF.
NRSF Binding (binding) of
7) Confidence 0.30 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0.05 Pain Relevance 0.11
end of NRSE and demonstrated that Sp3 transcription factor represses the expression of MOR gene by binding to this element and by interacting with NRSF.
NRSF Binding (interacting) of associated with mu opioid receptor
8) Confidence 0.30 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0.05 Pain Relevance 0.46
We have also reported that MOR transcription is suppressed by the neuron-restrictive silencer element (NRSE) in the mouse MOR promoter through binding of neuron-restriction silence factor (NRSF) (11).
NRSF Binding (binding) of in neuron associated with mu opioid receptor
9) Confidence 0.27 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0 Pain Relevance 0.60
The signal left after the treatment of the mutated competitor represents the complex with either NRSF (lane 7) or Sp3 (lane 8).
NRSF Binding (represents) of
10) Confidence 0.27 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0 Pain Relevance 0.03
Previously, we reported that the MOR transcription is suppressed by NRSE in the mouse MOR promoter through binding of NRSF (11).
NRSF Binding (binding) of associated with mu opioid receptor
11) Confidence 0.27 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0 Pain Relevance 0.23
Although the exact mechanism of interdependency (or cooperativity) between NRSF and Sp3 for binding to NRSE/GC box remains to be elucidated, it is tempting to speculate that the function of Sp3 could be determined by the interaction with NRSF.
NRSF Spec (determined) Binding (interaction) of
12) Confidence 0.26 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0.12 Pain Relevance 0.10
In this study, we demonstrated that Sp3 transcription factor represses the MOR gene expression by binding to a GC box adjacent to NRSE and by interacting with NRSF providing evidence that there is another level of complexity in regulating the MOR transcription.
NRSF Binding (interacting) of associated with mu opioid receptor
13) Confidence 0.26 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1702488 Disease Relevance 0.62 Pain Relevance 0.17
For example, NRSF (neuron-restrictive silencer factor) can bind to a 21 bp NRSE element in the proximal promoter region to suppress the promoter activity [31].
NRSF Binding (bind) of in neuron
14) Confidence 0.13 Published 2006 Journal BMC Mol Biol Section Body Doc Link PMC1657025 Disease Relevance 0.12 Pain Relevance 0.18
appaB, SOX, and neuron-restrictive silencer element (NRSE) in the proximal or distal promoters have been identified to interact with their trans-acting partners, which positively or negatively regulate the E1 promoter activity [29-42].
neuron-restrictive silencer element Binding (interact) of in proximal
15) Confidence 0.13 Published 2006 Journal BMC Mol Biol Section Body Doc Link PMC1657025 Disease Relevance 0 Pain Relevance 0.20
In the non-neuronal cells, repressor element 1 (RE-1) silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF) represses the vasopressin gene expression by the direct binding of a neuron-restrictive silencer element (NRSE) (Ballas et al. 2005).
neuron-restrictive silencer element Binding (binding) of in neuron
16) Confidence 0.02 Published 2008 Journal Gene Regulation and Systems Biology Section Body Doc Link PMC2733102 Disease Relevance 0.09 Pain Relevance 0

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