INT199692

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Context Info
Confidence 0.75
First Reported 2006
Last Reported 2010
Negated 4
Speculated 1
Reported most in Body
Documents 48
Total Number 59
Disease Relevance 15.20
Pain Relevance 3.84

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

ligase activity (Mkrn1)
Anatomy Link Frequency
monocytes 13
macrophages 4
brain 4
spinal 2
HeLa 2
Mkrn1 (Mus musculus)
Pain Link Frequency Relevance Heat
Central nervous system 85 99.16 Very High Very High Very High
Inflammation 793 98.80 Very High Very High Very High
IPN 83 98.32 Very High Very High Very High
imagery 176 98.04 Very High Very High Very High
chemokine 252 98.02 Very High Very High Very High
Spinal cord 110 97.16 Very High Very High Very High
halothane 4 92.56 High High
intrathecal 36 92.48 High High
Multiple sclerosis 72 87.36 High High
Inflammatory mediators 36 84.64 Quite High
Disease Link Frequency Relevance Heat
Multiple Sclerosis 1620 99.84 Very High Very High Very High
Disease 666 99.56 Very High Very High Very High
Viral Meningitis 36 99.16 Very High Very High Very High
INFLAMMATION 833 98.80 Very High Very High Very High
Cancer 18 98.16 Very High Very High Very High
Peritonitis 252 98.00 Very High Very High Very High
Targeted Disruption 500 96.80 Very High Very High Very High
Disorder Of Lipid Metabolism 36 96.64 Very High Very High Very High
Sprains And Strains 50 89.92 High High
Acquired Immune Deficiency Syndrome Or Hiv Infection 139 89.16 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To determine if individual axons are likely to innervate both L and R sides of the IPN, we expressed, by focal electroporation in individual neurons, a construct driving expression of both cytoplasmic red fluorescent protein (RFP) and a Synaptophysin-GFP (Syp-GFP) fusion protein that localizes to presynaptic terminals [17].
Gene_expression (expression) of RFP in neurons associated with ipn
1) Confidence 0.75 Published 2008 Journal Neural Develop Section Body Doc Link PMC2292717 Disease Relevance 0 Pain Relevance 0.41
Furthermore, CCR2-RFP expression did not overlap with markers of astrocytes, neurons, or oligodendrocytes (data not shown).
Gene_expression (expression) of RFP in astrocytes
2) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.28 Pain Relevance 0.14
After instillation of thioglycollate for 24 h to induce peritonitis, Ccr2RFP/+Cx3cr1GFP/+ F4/80dim monocytes were actively recruited to the peritoneum, and almost all expressed high levels of both RFP and GFP (Figure 3A).
Gene_expression (expressed) of RFP in peritoneum associated with peritonitis
3) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.25 Pain Relevance 0
Finally, RFP and GFP expression in infiltrated cells in the CNS overlapped with the patterns observed in the blood.
Gene_expression (expression) of RFP in blood
4) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.26 Pain Relevance 0.07
Flow cytometric analysis of Ccr2RFP/+ and Ccr2RFP/RFP peripheral blood cells stained for cell-surface CCR2 (Figure 1C) confirmed bi-allelic expression of CCR2 and RFP in heterozygous mice and deletion of CCR2 in Ccr2RFP/RFP homozygous mice.
Gene_expression (expression) of RFP in blood cells
5) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.18 Pain Relevance 0.03
Detailed analysis of RFP/GFP expression and cellular morphology in the lesion cores provided further insight into the functions of CCR2 and Ly6Chi monocytes in EAE.
Gene_expression (expression) of RFP in monocytes associated with multiple sclerosis
6) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.43 Pain Relevance 0.09
To visualize the distribution of cells expressing GFP (CX3CR1) and RFP (CCR2), free-floating sections were stained with 15 nM DAPI for 5 min, and whole-brain sections were scanned using a Leica DM5500B with motorized x,y stage.
Gene_expression (expressing) of RFP in brain
7) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.70 Pain Relevance 0.03
We created and characterized a transgenic mouse model that allows simultaneous tracking of two key chemokine receptors, CCR2 and CX3CR1 and enumeration of distinct monocyte and myeloid subsets based on CCR2-RFP/CX3CR1-GFP expression.
Gene_expression (expression) of RFP in monocyte associated with targeted disruption and chemokine
8) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.41 Pain Relevance 0.11
At high magnification, RFP expression was found in occasional perivascular cells in healthy Ccr2RFP/+Cx3cr1GFP/+ brains (Figures 5A and D); flow cytometry, these RFP+ cells accounted for <2% of the CD45+ marrow-derived cells in naive brain (data not shown).
Gene_expression (expression) of RFP in brain
9) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.14 Pain Relevance 0
Otherwise RFP expression was absent from the parenchyma of healthy brains (Figure 5D and not shown).
Neg (absent) Gene_expression (expression) of RFP in brains
10) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.30 Pain Relevance 0.03
RFP and CCR2 RNA expression in leukocytes
Gene_expression (expression) of RFP in leukocytes
11) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.06 Pain Relevance 0
Injections of PRV152-EGFP and/or PRV614-RFP were made in multiple sites in both crural and costal regions of the muscle (Fig. 1A) using 10 ┬Ál Hamilton syringe equipped with a 33 gauge needle (Hamilton; Reno, NV).
Gene_expression (Injections) of RFP in muscle
12) Confidence 0.65 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2909228 Disease Relevance 0 Pain Relevance 0.05
Comparison of CX3CR1-GFP and CCR2-RFP fluorescence intensities in the various CD115+ populations from Ccr2RFP/+Cx3cr1GFP/+ mice showed that Ly6Chi cells expressed higher levels of CCR2-RFP (P?
Gene_expression (expressed) of RFP
13) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.36 Pain Relevance 0.06
At 24 h, the vast majority of monocyte/macrophages recruited to the peritoneum in Ccr2+/RFPCx3cr1+/GFP mice expressed RFP and GFP.
Gene_expression (expressed) of RFP in macrophages
14) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.77 Pain Relevance 0.18
We next examined RFP expression and CCR2 surface staining on monocytes stratified by their level of Ly6C.
Spec (examined) Gene_expression (expression) of RFP in monocytes
15) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.13 Pain Relevance 0.04
Both Ly6Chi and Ly6Clo monocytes were RFP positive, but Ly6Chi cells were brighter (Figure 2I).
Gene_expression (positive) of RFP in monocytes
16) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.15 Pain Relevance 0.04
Under the steady-state, low-level inflammatory conditions of a high-fat diet, Ly6Chi monocytes had higher RFP intensity and lower GFP intensity than Ly6Clo cells, allowing the subsets to be clearly distinguished by their RFP/GFP profiles.
Gene_expression (profiles) of RFP in monocytes associated with inflammation
17) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.67 Pain Relevance 0.18
In naive mice, the vast majority of F4/80hi peritoneal macrophages were negative for GFP and RFP and were probably resident macrophages (Figure 3A).
Neg (negative) Gene_expression (negative) of RFP in macrophages
18) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.19 Pain Relevance 0
We used 7/4 as a surrogate for Ly6C staining in brain sections and found a corresponding correlation between the presence of RFP and GFP depending on the 7/4 status of the monocyte (Figure 6).
Gene_expression (presence) of RFP in brain
19) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.38 Pain Relevance 0.03
Comparison of CX3CR1-GFP and CCR2-RFP fluorescence intensities in the various CD115+ populations from Ccr2RFP/+Cx3cr1GFP/+ mice showed that Ly6Chi cells expressed higher levels of CCR2-RFP (P?
Gene_expression (intensities) of RFP
20) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965160 Disease Relevance 0.36 Pain Relevance 0.06

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