INT200833

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Context Info
Confidence 0.73
First Reported 2006
Last Reported 2008
Negated 0
Speculated 0
Reported most in Body
Documents 2
Total Number 20
Disease Relevance 5.56
Pain Relevance 2.90

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (Ndel1) transport (Ndel1) nuclear envelope (Ndel1)
chromosome segregation (Ndel1) chromosome (Ndel1) cytoskeleton (Ndel1)
Anatomy Link Frequency
neuronal 5
neurons 3
nerves 3
sciatic nerve 1
proximal 1
Ndel1 (Mus musculus)
Pain Link Frequency Relevance Heat
dorsal root ganglion 361 100.00 Very High Very High Very High
Sciatic nerve 589 98.00 Very High Very High Very High
Spinal cord 190 97.56 Very High Very High Very High
anesthesia 20 5.00 Very Low Very Low Very Low
butorphanol 19 5.00 Very Low Very Low Very Low
Analgesic 19 5.00 Very Low Very Low Very Low
Dorsal column 19 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Ganglion Cysts 418 100.00 Very High Very High Very High
Injury 400 99.96 Very High Very High Very High
Embryonic Lethality 38 87.52 High High
Nervous System Injury 38 86.72 High High
Teratoma 10 86.68 High High
Sprains And Strains 3 73.60 Quite High
Infection 19 51.92 Quite High
Targeted Disruption 57 39.40 Quite Low
Neuroblastoma 19 30.12 Quite Low
Coronary Artery Disease 57 27.32 Quite Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
We took advantage of the neuronal pattern of expression of Ndel1 and performed co-immunoprecipitations on the preparations with Ndel1 antibodies in detergent-free buffers (see Material and Methods).
Gene_expression (expression) of Ndel1 in neuronal
1) Confidence 0.73 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.30 Pain Relevance 0.46
Approximately 60% of cultured DRG neurons expressed Vimentin while nearly all Vimentin-positive DRG neurons express Ndel1.
Gene_expression (express) of Ndel1 in neurons associated with dorsal root ganglion
2) Confidence 0.73 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.69 Pain Relevance 0.32
Double in situ hybridization/immunohistochemistry with NF antibody further confirmed that Ndel1 mRNA was strongly expressed in DRG neurons (Fig. 1C).
Gene_expression (expressed) of Ndel1 mRNA in neurons associated with dorsal root ganglion
3) Confidence 0.73 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.51 Pain Relevance 0.43
The control (scrambled) siRNA or Ndel1 siRNA were commercially produced according to the manufacturer's protocol, with a fluorescent tag placed at the 3?
Gene_expression (produced) of Ndel1 siRNA
4) Confidence 0.73 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.21 Pain Relevance 0.08
These data may suggest heterogeneity in the molecular composition of the Ndel1/Vimentin complex in cells.
Gene_expression (complex) of Ndel1/Vimentin
5) Confidence 0.63 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.15 Pain Relevance 0.03
Importantly, in both proximal and distal segment of injured nerves, enhanced formation of neuronal Ndel1/Vimentin complex was observed after injury, as indicated by immunoprecipitations with Ndel1 antibodies (Fig. 2D).
Gene_expression (antibodies) of Ndel1 in distal segment associated with injury
6) Confidence 0.63 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.18 Pain Relevance 0.03
Local silencing of Ndel1 by siRNA in injured nerves reduces axon regeneration
Gene_expression (silencing) of Ndel1 in nerves
7) Confidence 0.63 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.51 Pain Relevance 0.26
Similar to the crush model, we observed synthesis and upregulation of Ndel1 and full length Vimentin in both injured distal and proximal nerve stumps when compared to the non-injured contralateral sides (control) at 65 hours following sciatic nerve transection (Fig. 3A and B).
Gene_expression (synthesis) of Ndel1 in sciatic nerve associated with sciatic nerve
8) Confidence 0.63 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0 Pain Relevance 0.14
Mechanisms of actions of Ndel1 during axon regeneration
Gene_expression (actions) of Ndel1
9) Confidence 0.63 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.22 Pain Relevance 0.04
Approximately 60% of cultured DRG neurons expressed Vimentin while nearly all Vimentin-positive DRG neurons express Ndel1.
Gene_expression (expressed) of Ndel1 in neurons associated with dorsal root ganglion
10) Confidence 0.63 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.70 Pain Relevance 0.33
To determine whether the Ndel1/Vimentin complex forms in injured axons, we again took advantage of the neuronal expression pattern of Ndel1 and performed co-immunoprecipitations with Ndel1 antibodies on sciatic nerve soluble lysates.
Gene_expression (expression) of Ndel1 in neuronal associated with sciatic nerve
11) Confidence 0.63 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.20 Pain Relevance 0.12
Highest levels of Ndel1/Vimentin in these co-immunoprecipitates were found in nerve samples with the highest degree of regeneration, as determined with levels of GAP-43 in total lysates (Fig. 2D).
Gene_expression (levels) of Ndel1/Vimentin in nerve
12) Confidence 0.63 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.17 Pain Relevance 0.03
To determine whether the Ndel1/Vimentin complex forms in injured axons, we again took advantage of the neuronal expression pattern of Ndel1 and performed co-immunoprecipitations with Ndel1 antibodies on sciatic nerve soluble lysates.
Gene_expression (expression) of Ndel1/Vimentin in neuronal associated with sciatic nerve
13) Confidence 0.63 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.21 Pain Relevance 0.17
The degree of augmentation in Ndel1 levels also correlated with higher levels of GAP-43, a neuronal marker for axonal regeneration, and with stabilization of NF-L, a marker for advanced regeneration and maturation (Fig. 2C).
Gene_expression (levels) of Ndel1 in neuronal
14) Confidence 0.63 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.22 Pain Relevance 0.07
Importantly, the Ndel1 siRNA reduced by 50% the levels of Ndel1 protein indicated by western blots (Fig. 6C and D).
Gene_expression (levels) of Ndel1 siRNA
15) Confidence 0.57 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.24 Pain Relevance 0.03
Ndel1 was synthesized de novo and upregulated in crushed and transected sciatic nerve axons, and, upon injury, was strongly associated with neuronal form of the intermediate filament (IF) Vimentin while dissociating from the mature neuronal IF (Neurofilament) light chain NF-L.
Gene_expression (synthesized) of Ndel1 in neuronal associated with injury and sciatic nerve
16) Confidence 0.57 Published 2008 Journal PLoS ONE Section Abstract Doc Link PMC2291557 Disease Relevance 0.32 Pain Relevance 0.05
Importantly, the Ndel1 siRNA reduced by 50% the levels of Ndel1 protein indicated by western blots (Fig. 6C and D).
Gene_expression (levels) of Ndel1 protein
17) Confidence 0.57 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.24 Pain Relevance 0.03
From 3 and 12 hours after injury, Ndel1 and Vimentin mRNAs and soluble proteins were synthesized and up-regulated in both distal and proximal stumps of injured nerves, when compared to the contralateral non-injured sides (Fig. 2).
Gene_expression (synthesized) of Ndel1 in nerves associated with injury
18) Confidence 0.57 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.16 Pain Relevance 0.24
Importantly, in both proximal and distal segment of injured nerves, enhanced formation of neuronal Ndel1/Vimentin complex was observed after injury, as indicated by immunoprecipitations with Ndel1 antibodies (Fig. 2D).
Gene_expression (antibodies) of Ndel1 in proximal associated with injury
19) Confidence 0.22 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2291557 Disease Relevance 0.18 Pain Relevance 0.03
cells that constituted 1%–10% of EB-derived cultures generated an E-RoSH-like culture with an identical E-RoSH-like gene expression profile (r2?
Gene_expression (expression) of E-RoSH-like gene
20) Confidence 0.03 Published 2006 Journal PLoS ONE Section Abstract Doc Link PMC1762397 Disease Relevance 0.16 Pain Relevance 0

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