INT201438

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Context Info
Confidence 0.68
First Reported 2006
Last Reported 2009
Negated 0
Speculated 0
Reported most in Body
Documents 3
Total Number 23
Disease Relevance 0.79
Pain Relevance 4.19

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

aging (Slc6a3) plasma membrane (Slc6a3) transmembrane transport (Slc6a3)
Slc6a3 (Rattus norvegicus)
Pain Link Frequency Relevance Heat
Kinase C 315 99.98 Very High Very High Very High
Dopamine 727 99.76 Very High Very High Very High
gABA 42 98.40 Very High Very High Very High
monoamine 21 85.24 High High
Nucleus accumbens 21 57.24 Quite High
Neuropeptide 42 33.16 Quite Low
Neurotransmitter 50 5.00 Very Low Very Low Very Low
addiction 42 5.00 Very Low Very Low Very Low
cocaine 23 5.00 Very Low Very Low Very Low
Serotonin 23 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Breast Cancer 4 83.92 Quite High
Disease 75 81.08 Quite High
Cancer 4 76.88 Quite High
Obesity 42 76.56 Quite High
Brain Injury 2 70.88 Quite High
Cognitive Disorder 27 63.52 Quite High
Volume Depletion And Dehydration 2 9.96 Low Low
Stress 63 5.00 Very Low Very Low Very Low
Drug Dependence 42 5.00 Very Low Very Low Very Low
Schizophrenia 23 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To explore this possibility, we first tested whether CPE had any effect on the phosphrylation levels of DAT.
Phosphorylation (phosphrylation) of DAT
1) Confidence 0.68 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.15
HZ carried out all experiments, with the exception of the in vitro binding, the uptake assay, WIN binding and the assays of the phosphorylation level of DAT.
Phosphorylation (phosphorylation) of DAT
2) Confidence 0.68 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0.16 Pain Relevance 0.07
Alternatively, there is evidence that PKC- mediated functional regulation of DAT is not achieved by direct phosphorylation of the DAT protein [58], as has been demonstrated for the GABA transporter, where PKC down regulates transporter function by modulating the association of syntaxin with the transporter [59].
Phosphorylation (phosphorylation) of DAT protein associated with gaba and kinase c
3) Confidence 0.68 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.35
The association of DAT with CPE may have critical impact on DAT phosphorylation state and be involved in cyclic trafficking.
Phosphorylation (phosphorylation) of DAT
4) Confidence 0.68 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0.13 Pain Relevance 0.17
The exact mechanism for CPE's effects on DAT phosphorylation and its relationship with cell surface DAT expression require further investigation.
Phosphorylation (phosphorylation) of DAT
5) Confidence 0.68 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.23
Reduced phosphorylation state of DAT might reflect that association of CPE with DAT leaded to the occlusion of PKC phosphorylation sites.
Phosphorylation (phosphorylation) of DAT associated with kinase c
6) Confidence 0.68 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.19
This is in agreement with our studies in which CPE decreases DAT phosphorylation leading to slower degradation and increase cell surface localization.
Phosphorylation (phosphorylation) of DAT
7) Confidence 0.68 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.20
SL conducted the immunoassays of the phosphorylation level of DAT and contribute to the writing of the manuscript.
Phosphorylation (phosphorylation) of DAT
8) Confidence 0.68 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0.15 Pain Relevance 0.08
Foster and colleagues recently report that the amino terminus of DAT, which contains a cluster of serine residues, is the primary site of phosphorylation by PKC [35].
Phosphorylation (phosphorylation) of DAT associated with kinase c
9) Confidence 0.67 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.31
This phosphorylation/dephosphorylation status is closely coupled to DAT endocytic trafficking and interestingly PKC activation has been shown to decrease DAT activity through clathrin-mediated endocytosis [31,55], an event associated with an increase in DAT phosphorylation [52].
Phosphorylation (phosphorylation) of DAT associated with kinase c
10) Confidence 0.59 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.20
However, there have been reports that PKC-induced phosphorylation of the DAT itself is not required for PKC-induced DAT sequestration [56], leading to the possibility that DAT are trapped in its phosphorylated state in subcellular compartment, where CPE may speed up the assorting and recycling back to the membrane of the internalized DAT.
Phosphorylation (phosphorylation) of DAT associated with kinase c
11) Confidence 0.59 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.23
We have shown that this interaction leads to cell surface stabilization of DAT via reduced degradation, possibly through a reduction in DAT phosphorylation.
Phosphorylation (phosphorylation) of DAT
12) Confidence 0.59 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.18
CPE stablized DAT via reducing the phosphorylation state of DAT
Phosphorylation (phosphorylation) of DAT
13) Confidence 0.59 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.08
It is possible that the interaction with CPE may mask the PKC phosphorylation site on DAT.
Phosphorylation (phosphorylation) of DAT associated with kinase c
14) Confidence 0.59 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.32
However, there have been reports that PKC-induced phosphorylation of the DAT itself is not required for PKC-induced DAT sequestration [56], leading to the possibility that DAT are trapped in its phosphorylated state in subcellular compartment, where CPE may speed up the assorting and recycling back to the membrane of the internalized DAT.
Phosphorylation (phosphorylated) of DAT associated with kinase c
15) Confidence 0.58 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.29
As shown in Fig 4B, PMA treatment reduced the association between CPE and DAT as compared with non-treated COS-7 cells, implying that CPE preferentially interacted with non-PKC phosphorylated forms of DAT.


Phosphorylation (phosphorylated) of DAT associated with kinase c
16) Confidence 0.52 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.26
To provide further evidence that the expression and functional affects on DAT were mediated by phosphorylation changes induced by the CPE-DAT interaction, we explored the relationship of DAT phosphorylation with DA translocation velocity and surface expression under phosphorylating conditions mentioned above.
Phosphorylation (phosphorylation) of DAT associated with dopamine
17) Confidence 0.52 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.21
In addition, agents that cause phosphorylation of DAT may regulate their trafficking by sequestration to particular intracellular compartments [50,67,68].
Phosphorylation (phosphorylation) of DAT
18) Confidence 0.47 Published 2006 Journal J Mol Signal Section Body Doc Link PMC1769494 Disease Relevance 0.27 Pain Relevance 0.05
Plate immunoassay for detection/quantitation of DAT and Ers
Phosphorylation (quantitation) of DAT
19) Confidence 0.46 Published 2006 Journal J Mol Signal Section Body Doc Link PMC1769494 Disease Relevance 0.08 Pain Relevance 0
We then examined the association between CPE and DAT under various phosphorylation conditions.
Phosphorylation (phosphorylation) of DAT
20) Confidence 0.45 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.20

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