INT202352

Context Info Confidence 0.69 First Reported 2007 Last Reported 2007 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 8 Disease Relevance 0.52 Pain Relevance 0

protein complex (Ccm2) cytoplasm (Ccm2)

Anatomy Link Frequency embryo 5 Ccm2 (Mus musculus)

Pain Link Frequency Relevance Heat cytokine 16 5.00 depression 8 5.00 cINOD 8 5.00 imagery 8 5.00

Disease Link Frequency Relevance Heat Stress 64 99.40 Congenital Anomalies 16 72.36 Reprotox - General 3 8 54.96 Increased Venous Pressure Under Development 32 5.00 Depression 8 5.00 Shock 8 5.00 INFLAMMATION 8 5.00 Carcinoma 8 5.00

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Event

Anatomy

Negation

Speculation

Pain term

Disease term

These results suggest a solute specificity in the induction of CCM2, which agrees with the induction of p38 MAPK activity and phosphorylation of MAPKAPK2 described above.

Positive_regulation (induction) of CCM2

1) Confidence 0.69 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC1781062 Disease Relevance 0.05 Pain Relevance 0

Though changes were not detected in localization or steady state mRNA transcript levels of CCM2 following hyperosmotic treatment, our results did reveal a significant increase in CCM2 immunofluorescence in blastocysts treated for 15 minutes in 0.2 M sorbitol (460 mOsm), but not 1.4% glycerol (460 mOsm).

Positive_regulation (increase) of CCM2

2) Confidence 0.60 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC1781062 Disease Relevance 0.06 Pain Relevance 0

An alternative explanation for this increase in detectable CCM2 protein may be in the regulation of scaffold protein stability.

Positive_regulation (increase) of CCM2 protein

3) Confidence 0.46 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC1781062 Disease Relevance 0 Pain Relevance 0

While our study has presented a novel discovery in the induction of CCM2 in response to hyperosmotic treatment of preimplantation embryos, much work remains to fully characterize CCM2 activity and regulation during this period of development.

Positive_regulation (induction) of CCM2 in embryos

4) Confidence 0.46 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC1781062 Disease Relevance 0.06 Pain Relevance 0

Embryo culture in hyperosmotic media increased p38 MAPK activity in conjunction with elevated CCM2 levels.

Positive_regulation (elevated) of CCM2 in Embryo

5) Confidence 0.46 Published 2007 Journal BMC Dev Biol Section Abstract Doc Link PMC1781062 Disease Relevance 0.07 Pain Relevance 0

These results define the expression of upstream activators of p38 MAPK during preimplantation development and indicate that embryo responses to hyperosmotic environments include elevation of CCM2 and activation of p38 MAPK.

Positive_regulation (activation) of CCM2 in embryo

6) Confidence 0.46 Published 2007 Journal BMC Dev Biol Section Abstract Doc Link PMC1781062 Disease Relevance 0.06 Pain Relevance 0

We have discovered that p38 MAPK activity is regulated by exposure to hyperosmotic stimuli, and that the response to hyperosmotic stress in the early embryo includes increased CCM2 levels.

Positive_regulation (increased) of CCM2 in embryo associated with stress

7) Confidence 0.46 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC1781062 Disease Relevance 0.15 Pain Relevance 0

These results define the expression of upstream activators of p38 MAPK during preimplantation development and indicate that embryo responses to hyperosmotic environments include elevation of CCM2 and activation of p38 MAPK.

Positive_regulation (elevation) of CCM2 in embryo

8) Confidence 0.46 Published 2007 Journal BMC Dev Biol Section Abstract Doc Link PMC1781062 Disease Relevance 0.06 Pain Relevance 0

General Comments

This test has worked.