INT205415

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Context Info
Confidence 0.75
First Reported 2007
Last Reported 2010
Negated 1
Speculated 1
Reported most in Body
Documents 17
Total Number 18
Disease Relevance 3.60
Pain Relevance 0.42

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytosol (Actb) cytoskeleton (Actb) protein complex (Actb)
cytoplasm (Actb)
Anatomy Link Frequency
kidney 2
neuronal 1
cornea 1
Actb (Mus musculus)
Pain Link Frequency Relevance Heat
Spinal cord 21 84.92 Quite High
cytokine 116 72.52 Quite High
ischemia 40 71.72 Quite High
chemokine 25 67.44 Quite High
imagery 17 66.56 Quite High
amygdala 4 61.76 Quite High
alcohol 13 52.88 Quite High
Analgesic 213 50.00 Quite Low
anesthesia 23 34.88 Quite Low
ketamine 7 20.88 Low Low
Disease Link Frequency Relevance Heat
Injury 175 99.54 Very High Very High Very High
Keratitis 65 99.16 Very High Very High Very High
Corneal Neovascularization 10 98.56 Very High Very High Very High
Aspergillus Infection 15 95.40 Very High Very High Very High
Candida Infection 15 94.40 High High
Infection 13 93.52 High High
Chemical Burns 25 92.64 High High
Apoptosis 93 89.16 High High
Corneal Disease 35 88.88 High High
Disease 32 87.56 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
For normalization of the data the average of the expression level of four house-keeping genes, GAPDH, HPRT, beta-actin and beta-tubulin, was used.



Gene_expression (expression) of beta-actin
1) Confidence 0.75 Published 2007 Journal BMC Genomics Section Body Doc Link PMC1829400 Disease Relevance 0 Pain Relevance 0
Expression of ACTB also changed in all models, though to a lesser extent than GAPDH.
Gene_expression (Expression) of ACTB
2) Confidence 0.73 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2893048 Disease Relevance 0.25 Pain Relevance 0
Primers were also designed for the reference genes GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) and ACTB (Actin, beta); all primer sequences are available in the Supplementary Material (see Additional file1).
Gene_expression (designed) of ACTB
3) Confidence 0.65 Published 2007 Journal Behav Brain Funct Section Body Doc Link PMC1858698 Disease Relevance 0 Pain Relevance 0.03
To perform real-time qPCR utilizing a LightCycler 480 (Roche Diagnostics K.K.), TaqMan® Gene Expression Assays (Life Technologies Japan Ltd.) were used as a probe/primer set specified for the KCNJ6 gene (Assay ID: Hs01040524_m1) and a probe/primer set for the ACTB gene, a house-keeping gene, encoding ?
Gene_expression (gene) of ACTB
4) Confidence 0.65 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2738941 Disease Relevance 0 Pain Relevance 0
The expression level of the KCNJ6 gene was normalized to that of the ACTB gene for each sample, and relative KCNJ6 mRNA expression levels between all samples were compared by setting the lowest expression level among all SMRI samples as 1.
Gene_expression (expression) of ACTB
5) Confidence 0.65 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2738941 Disease Relevance 0 Pain Relevance 0
The expression level of the KCNJ6 gene was normalized to that of the ACTB gene for each sample, and relative KCNJ6 mRNA expression levels between all samples were compared by setting the lowest expression level among all SMRI samples as 1.
Gene_expression (expression) of ACTB
6) Confidence 0.65 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2738941 Disease Relevance 0 Pain Relevance 0
In the few genes that were up-regulated (Actb, Atf3, Frs2, Hspb1, Nrap, Stat1) post-axotomy, the expression for all but one was down-regulated by 2-weeks post-injury.
Gene_expression (expression) of Actb associated with injury
7) Confidence 0.58 Published 2010 Journal BMC Neurosci Section Abstract Doc Link PMC3001741 Disease Relevance 0.83 Pain Relevance 0.10
-actin (Actb) expression chosen as control.


Gene_expression (expression) of Actb
8) Confidence 0.58 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2956702 Disease Relevance 0 Pain Relevance 0.03
-actin (Actb) expression chosen as control.


Gene_expression (expression) of Actb
9) Confidence 0.58 Published 2010 Journal Molecular Vision Section Body Doc Link PMC3013064 Disease Relevance 0.22 Pain Relevance 0.09
To account for variation in mRNA quantity, the geometric mean of the expression of the two reference genes ACTB and GAPDH [38] were included as the covariate (G) in the model [39].
Gene_expression (expression) of ACTB
10) Confidence 0.58 Published 2007 Journal Behav Brain Funct Section Body Doc Link PMC1858698 Disease Relevance 0 Pain Relevance 0
However, it has been gradually realized that all HKGs, especially those traditional HKGs selected based on PCR experiments of earlier days, like beta-actin (ACTB) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are not necessarily expressed at stable levels in all tissues/cells under all conditions [1-5].
Neg (not) Gene_expression (expressed) of ACTB
11) Confidence 0.56 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2893048 Disease Relevance 0 Pain Relevance 0.07
Expression of 8 HKGs (glyceraldehyde-3-phosphate dehydrogenase [GAPDH], beta-actin [ACTB], lactate dehydrogenase A [LDHA], ribosomal protein L5 [RPL5], ubiquitin C [UBC], peptidylprolyl isomerase A [PPIA], TATA-box binding protein [TBP1], and hypoxanthine guanine phosphoribosyl transferase [HPRT1]) in the cornea were measured at various time points by microarray hybridization or qRT–PCR and the data analyzed using geNorm and NormFinder.


Spec (analyzed) Gene_expression (Expression) of ACTB in cornea
12) Confidence 0.56 Published 2010 Journal Molecular Vision Section Abstract Doc Link PMC2893048 Disease Relevance 0.84 Pain Relevance 0
In this paper, we establish models of experimental corneal neovascularization (CorNV), fungal keratitis (FK) and corneal wound in mice and study the expression patterns of three traditional HKGs (ACTB, lactate dehydrogenase [LDHA], and GAPDH), five new HKGs (ubiquitin C [UBC] [14], peptidylprolyl isomerase A [PPIA] [15], TATA-box binding protein [TBP] [16], hypoxanthine guanine phosphoribosyl transferase [HPRT1] [17], and ribosomal protein L5 [RPL5] [18]).
Gene_expression (expression) of ACTB associated with corneal neovascularization, injury and keratitis
13) Confidence 0.56 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2893048 Disease Relevance 0.40 Pain Relevance 0.04
Among the total of 37 papers published in IOVS or Molecular Vision between the years 2000 and 2009 that address CorNV or FK using RT–PCR, 22 papers used GAPDH, 14 papers used ACTB, and 1 paper used 18s rRNA as HKG for normalization (detailed list of papers not shown).
Gene_expression (used) of ACTB associated with keratitis
14) Confidence 0.55 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2893048 Disease Relevance 0.22 Pain Relevance 0
Both the luciferase gene and a human housekeeping gene ACTB (both expressed only in human cells) were present only in the injected kidney tissue, as confirmed by the absence of the both housekeeping ACTB and luciferase gene in non-injected kidneys (Figure 3D).
Gene_expression (present) of ACTB in kidney
15) Confidence 0.44 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2827539 Disease Relevance 0 Pain Relevance 0.03
Both the luciferase gene and a human housekeeping gene ACTB (both expressed only in human cells) were present only in the injected kidney tissue, as confirmed by the absence of the both housekeeping ACTB and luciferase gene in non-injected kidneys (Figure 3D).
Gene_expression (expressed) of ACTB in kidney
16) Confidence 0.44 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2827539 Disease Relevance 0 Pain Relevance 0.03
Survival related gene, Voltage dependent anion channel (Vdac1) and ubiquitin related gene, ubiquitin B (Ubb) and genes involved in localized neuronal translation, cytoplasmic beta-actin (Actb), eukaryotic elongation factor2 (Eef2) and eukaryotic elongation factor 1 (Eef1alpha 1); retrograde transport, p25 subunit of dynactin (Dctn5), were amongst the genes that showed significant upregulation in 59Q transfected cells as compared to internally normalized expression levels in vector transfected controls (Fig. 1).
Gene_expression (expression) of Actb in neuronal
17) Confidence 0.26 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.35 Pain Relevance 0
Normalization was done using ACTB and HSPE as endogenous controls.
Gene_expression (using) of ACTB
18) Confidence 0.08 Published 2010 Journal Cancer Cell Int Section Body Doc Link PMC3004887 Disease Relevance 0.49 Pain Relevance 0

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