INT207804

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Context Info
Confidence 0.59
First Reported 2007
Last Reported 2008
Negated 0
Speculated 2
Reported most in Body
Documents 2
Total Number 14
Disease Relevance 6.22
Pain Relevance 0.09

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleus (Atm) cell cycle (Atm) DNA binding (Atm)
kinase activity (Atm) cytoplasm (Atm)
Anatomy Link Frequency
endothelial cells 2
M cell 2
Atm (Mus musculus)
Pain Link Frequency Relevance Heat
anesthesia 14 94.32 High High
Disease Link Frequency Relevance Heat
Apoptosis 341 97.20 Very High Very High Very High
Telangiectasia 18 96.36 Very High Very High Very High
Dna Damage 1 95.28 Very High Very High Very High
Solid Tumor 104 94.76 High High
Squamous Cell Carcinoma 52 94.60 High High
Female Infertility 1 92.92 High High
Ataxia 29 91.68 High High
Lymphatic System Cancer 1 90.80 High High
Cancer 355 83.80 Quite High
Hypersensitivity 13 81.32 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Then we investigated whether the reduction of ATM expression resulted in radiosensitization in SCCVII cells.
Spec (whether) Negative_regulation (reduction) of Gene_expression (expression) of ATM
1) Confidence 0.59 Published 2008 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2584003 Disease Relevance 0.20 Pain Relevance 0
From flow cytometry, we found that the cells did not accumulate in the G2/M phase following irradiation in cells transfected with ATM AS-ODNs, which mean the reduced ATM expression resulted in the defective G2/M checkpoint control.
Negative_regulation (reduced) of Gene_expression (expression) of ATM
2) Confidence 0.59 Published 2008 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2584003 Disease Relevance 0.51 Pain Relevance 0
We found that expression of ATM was dramatically reduced after cells were transfected with ATM AS-ODNs compared with that Sen-ODNs and Mis-ODNs treated groups, which indicated that the inhibition was specific for the ATM antisense sequence.
Negative_regulation (reduced) of Gene_expression (expression) of ATM
3) Confidence 0.59 Published 2008 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2584003 Disease Relevance 0.17 Pain Relevance 0
A 25-mer AS-ODN which was previously reported to inhibit ATM expression in mouse cerebrovascular endothelial cells[17], and its associated controls, sense (Sen) and mismatch (Mis) ODNs, were synthesized by Shanghai Sangon Biological Engineering Technology & Services (Shangai, China).
Negative_regulation (inhibit) of Gene_expression (expression) of ATM in endothelial cells
4) Confidence 0.59 Published 2008 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2584003 Disease Relevance 0.07 Pain Relevance 0
Therefore, we manage to examine whether reduction of ATM expression after antisense oligodeoxynucleotides (AS-ODNs) treatment would result in enhanced radiosensitivity of p53-mutated SCCVII cells from C3H/He mice through the aberrant G2/M checkpoint.


Spec (whether) Negative_regulation (reduction) of Gene_expression (expression) of ATM
5) Confidence 0.51 Published 2008 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2584003 Disease Relevance 0.44 Pain Relevance 0
Inhibition of ATM expression sensitized SCCVII cells to ionizing radiation in vitro and in vivo.
Negative_regulation (Inhibition) of Gene_expression (expression) of ATM
6) Confidence 0.43 Published 2008 Journal J Exp Clin Cancer Res Section Abstract Doc Link PMC2584003 Disease Relevance 0.53 Pain Relevance 0
The potential mechanism of radiosensitization related with reduced ATM expression should be the defective G2/M cell-cycle checkpoint control and enhanced radiation-induced apoptosis.


Negative_regulation (reduced) of Gene_expression (expression) of ATM in M cell associated with apoptosis
7) Confidence 0.43 Published 2008 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2584003 Disease Relevance 0.80 Pain Relevance 0
As shown in Figure. 2, ATM protein expression was also significantly reduced by ATM AS-ODNs compared with Sen-ODNs and Mis-ODNs after 72 hours treatment (Figure. 2A).
Negative_regulation (reduced) of Gene_expression (expression) of ATM
8) Confidence 0.43 Published 2008 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2584003 Disease Relevance 0.16 Pain Relevance 0
Our objective is to sensitize SCCVII cells to ionizing radiation in vitro and in vivo through inhibiting ATM expression using antisense oligodeoxynucleotides (AS-ODNs), and investigate the potential mechanism of radiosensitization.


Negative_regulation (inhibiting) of Gene_expression (expression) of ATM
9) Confidence 0.43 Published 2008 Journal J Exp Clin Cancer Res Section Abstract Doc Link PMC2584003 Disease Relevance 0.34 Pain Relevance 0
The relative ATM mRNA and protein expression in SCCVII cells treated with ATM AS-ODNs were decreased to 25.7 ± 3.1% and 24.1 ± 2.8% of that in untreated cells respectively (P < 0.05).
Negative_regulation (decreased) of Gene_expression (expression) of ATM mRNA
10) Confidence 0.43 Published 2008 Journal J Exp Clin Cancer Res Section Abstract Doc Link PMC2584003 Disease Relevance 0.63 Pain Relevance 0
However when incubating with liposome formulations of ATM AS-ODNs, the relative ATM mRNA expression was only about 25.7 ± 3.1% to the untreated SCCVII cells, which demonstrated a significantly reduced expression of ATM mRNA (P < 0.05; Figure. 1).
Negative_regulation (reduced) of Gene_expression (expression) of ATM mRNA
11) Confidence 0.43 Published 2008 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2584003 Disease Relevance 0.17 Pain Relevance 0
Functional inactivation of the ATM gene product and Atm-null mice, which were created by disrupting the Atm locus, recapitulate the human A-T phenotype and display growth retardation, mild neurological dysfunction, male and female infertility, extreme predisposition to thymic lymphomas, and acute sensitivity to ionizing radiation [1-3].
Negative_regulation (inactivation) of Gene_expression (product) of ATM associated with anesthesia, lymphatic system cancer, telangiectasia and female infertility
12) Confidence 0.43 Published 2007 Journal BMC Mol Biol Section Body Doc Link PMC1868035 Disease Relevance 0.83 Pain Relevance 0.09
We had demonstrated that the ATM AS-ODNs used in our study could specificly reduce the ATM expression and further result in an increased radiosensitivity in SCCVII cells in vitro and in vivo.
Negative_regulation (reduce) of Gene_expression (expression) of ATM
13) Confidence 0.38 Published 2008 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2584003 Disease Relevance 0.91 Pain Relevance 0
Therefore we designed the experiment to test the hypothesis whether ATM AS-ODNs could inhibit the expression of ATM in SCCVII cells and furthermore increase the radiosensitivity by enhancing radiation-induced apoptosis in vitro and in vivo.
Negative_regulation (inhibit) of Gene_expression (expression) of ATM associated with apoptosis
14) Confidence 0.38 Published 2008 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2584003 Disease Relevance 0.47 Pain Relevance 0

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