INT207902

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Context Info
Confidence 0.18
First Reported 2007
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 19
Total Number 19
Disease Relevance 0.93
Pain Relevance 0.04

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

mitochondrion (Mgst1) endoplasmic reticulum (Mgst1) nucleus (Mgst1)
Anatomy Link Frequency
liver 1
Mgst1 (Mus musculus)
Pain Link Frequency Relevance Heat
depression 10 81.56 Quite High
adenocard 90 50.00 Quite Low
Antinociceptive 66 50.00 Quite Low
Eae 3 46.60 Quite Low
cerebral cortex 18 32.64 Quite Low
Pain 7 28.64 Quite Low
anesthesia 14 25.04 Quite Low
agonist 16 17.24 Low Low
dorsal root ganglion 74 5.00 Very Low Very Low Very Low
Inflammation 15 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Poisoning 26 97.12 Very High Very High Very High
Infection 79 95.20 Very High Very High Very High
Depression 10 81.56 Quite High
Sprains And Strains 24 75.68 Quite High
Disease 168 53.16 Quite High
Stress 58 51.12 Quite High
Ganglion Cysts 79 50.00 Quite Low
Malaria 45 50.00 Quite Low
Nephrotoxicity 8 50.00 Quite Low
Body Weight 34 40.88 Quite Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
GST assay
GST Binding (assay) of
1) Confidence 0.18 Published 2007 Journal BMC Complement Altern Med Section Body Doc Link PMC1868763 Disease Relevance 0 Pain Relevance 0
GST activity (2.78 ± 0.04 ?
GST Binding (activity) of
2) Confidence 0.18 Published 2007 Journal BMC Complement Altern Med Section Body Doc Link PMC1868763 Disease Relevance 0.10 Pain Relevance 0
We reasoned that GST, a protein that binds glutathione, might interfere with physiological or behavioral studies if administered in vivo.
GST Binding (binds) of
3) Confidence 0.16 Published 2010 Journal Mol Pain Section Body Doc Link PMC2874211 Disease Relevance 0 Pain Relevance 0
Loading was performed overnight at a slow flow rate (0.4 mL/min. for 14-16 hours) to optimize binding of the GST-tagged protein.
GST Binding (binding) of
4) Confidence 0.16 Published 2010 Journal Mol Pain Section Body Doc Link PMC2874211 Disease Relevance 0 Pain Relevance 0
Cleaved mNT5E and thrombin were eluted in PBS while the GST tag remained bound to the column.
GST Binding (bound) of
5) Confidence 0.16 Published 2010 Journal Mol Pain Section Body Doc Link PMC2874211 Disease Relevance 0 Pain Relevance 0
However, as shown in Figure 7, the mutant and wild type N-terminal regions do differ in that they interact with different proteins, since the N-terminal GST-fusion protein for the Cav1.4wt sequence pulls down filamin protein, whereas the Cav1.4nob2 peptide does not.
GST-fusion Binding (interact) of
6) Confidence 0.13 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2432030 Disease Relevance 0 Pain Relevance 0
To isolate PRIC295 interacting proteins, nuclear extracts were allowed to interact with GST-?
GST Binding (interact) of
7) Confidence 0.11 Published 2010 Journal PPAR Research Section Body Doc Link PMC2946606 Disease Relevance 0 Pain Relevance 0
To identify which domains of Smad3 are required for the interaction, GST-pulldown assays were performed using deletion constructs for Smad3.
GST Binding (interaction) of
8) Confidence 0.10 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0
The bound fusion protein was eluted with 2 ml of GST elution buffer and stored at ?
GST Binding (bound) of
9) Confidence 0.10 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0 Pain Relevance 0
Cell lysates from these cells were mixed with glutathione-agarose bound GST-PlexinA1 cytoplasmic domain or GST as a control.
GST Binding (bound) of
10) Confidence 0.10 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
Cell lysates from these cells were mixed with glutathione-agarose bound GST-PlexinA1 cytoplasmic domain or GST as a control.
GST Binding (bound) of
11) Confidence 0.10 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
The supernatant was incubated with 2 ml 50% slurry of GST bind resin that had been washed three times with GST bind/wash buffer for 1 h and poured into a small column and washed with 10 ml GST bind/wash buffer.
GST Binding (bind) of
12) Confidence 0.10 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.11 Pain Relevance 0
The supernatant was incubated with 2 ml 50% slurry of GST bind resin that had been washed three times with GST bind/wash buffer for 1 h and poured into a small column and washed with 10 ml GST bind/wash buffer.
GST Binding (bind) of
13) Confidence 0.10 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.10 Pain Relevance 0
Although GST–nephrinICD2 can bind with the isolated first or third PDZ domains of PAR3, all three PDZ domains are required for efficient binding.
GST Binding (bind) of
14) Confidence 0.07 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2614475 Disease Relevance 0 Pain Relevance 0
Activity of GST and UGT, and levels of GSH and TBARS in the liver of P. berghei-infected mice
GST Binding (Activity) of in liver
15) Confidence 0.06 Published 2010 Journal Malar J Section Body Doc Link PMC2858213 Disease Relevance 0.63 Pain Relevance 0.04
We performed in vitro kinase assays on bacterially purified GST-BMAL1 or GST alone by incubating them with recombinant GSK3?
GST Binding (purified) of
16) Confidence 0.06 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2797305 Disease Relevance 0 Pain Relevance 0
T7-tagged PAR3 mutants overexpressed in 293T cells specifically interacted with GST–nephrinICD, except for the mutants lacking all PDZ domains or mutated in the third PDZ domain [27], [28], indicating the third PDZ domain is required for the formation of the complex with nephrin (Figure 6B).
GST Binding (interacted) of
17) Confidence 0.06 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2614475 Disease Relevance 0 Pain Relevance 0
We then analyzed the blots to determine if there were sequence-specific binding to any of the GST fusion proteins, which represented 29 different PDZ domains (Redwood, CA).
GST Binding (binding) of
18) Confidence 0.02 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2610370 Disease Relevance 0 Pain Relevance 0
To probe whether sphingosine can affect a preexisting synaptobrevin/membrane interaction, we followed membrane binding by GST-synaptobrevin (aa 1–96) in real time using a spectrophotometric assay.
GST Binding (binding) of
19) Confidence 0.02 Published 2009 Journal Neuron Section Body Doc Link PMC2697323 Disease Relevance 0 Pain Relevance 0

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