INT209019
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Mice were randomly assigned to either a control group (receiving PBS) or two treatment groups (receiving BCG or rBCG-S1PT). | |||||||||||||||
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Mice were randomly assigned to either a control group (receiving PBS) or two treatment groups (receiving BCG or rBCG-S1PT). | |||||||||||||||
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Others have found that BCG also releases IL-10 from the mouse bladder [37]. | |||||||||||||||
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BCG-induced cytokine release | |||||||||||||||
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Among the cytokines released by BCG, attention has been paid to IL-6 because its urinary levels are correlated with symptom scores in cystitis [50,51] and are reduced by BCG therapy in bladder cancer [52]. | |||||||||||||||
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In this first manuscript, we propose to determine whether the intact mouse urinary bladder takes up BCG, the time-dependent morphological alterations, and urinary cytokine release in response to intravesical BCG. | |||||||||||||||
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Co-localization of BCG-GFP with LRG47 in the mouse bladder urothelium | |||||||||||||||
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For co-localization of BCG-GFP and LRG47-Rhodamine, pictures were converted to 8-bit gray scale images (fluorescence intensity range: 0 < 255) using Image J software [23] and the analysis was performed with the co-localization-finder plug-in [24]. | |||||||||||||||
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Next, the co-localization of BCG-GFP and LRG47-Rhodamine was performed by converting the Figures 13B and 13C to 8-bit gray scale images (fluorescence intensity range: 0 < 255) using Image J software [23], assigned as red and green channels, and the analysis was performed with the co-localization-finder plug-in [24]. | |||||||||||||||
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Next, we used fluorescence immunohistochemistry to demonstrate that, in our laboratory conditions, instillation of BCG-GFP does not cause any microscopic extravasation of BCG or cause artifacts, including forcing bacteria into the urothelium. | |||||||||||||||
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Although this finding potentially linking BCG to axonal guidance is unique, activation of small GTPases genes by intravesical instillation of BCG into the mouse bladder was just demonstrated by our laboratory [25] and we extended the results into the present work, indicating that acute BCG does co-localize with LRG47 vesicles. | |||||||||||||||
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In the merged picture, presented in Figure 13D, it can be appreciated that most of background fluorescence coincides with DAPI staining (blue) whereas the BCG-GFP is co-localized in vesicles positives for LRG-47 (yellow). | |||||||||||||||
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For this purpose, we used the C57BL/6 mice because our previous work already defined the time-course of bladder inflammation and urinary cytokine release in response to intravesical instillation of BCG, TNF-? | |||||||||||||||
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General Comments
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