INT209508

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Context Info
Confidence 0.20
First Reported 2007
Last Reported 2007
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 18
Disease Relevance 7.28
Pain Relevance 0.30

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
spleen 2
PBL 2
hematopoietic cells 2
lymph nodes 1
liver 1
Plaa (Rattus norvegicus)
Pain Link Frequency Relevance Heat
tolerance 252 98.62 Very High Very High Very High
Osteoarthritis 18 45.48 Quite Low
ketamine 54 36.00 Quite Low
anesthesia 108 34.56 Quite Low
Versed 36 5.00 Very Low Very Low Very Low
Central nervous system 18 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Targeted Disruption 846 99.70 Very High Very High Very High
Sprains And Strains 72 99.08 Very High Very High Very High
Immunization 54 98.86 Very High Very High Very High
Injury 18 51.64 Quite High
Osteoarthritis 18 45.48 Quite Low
Exsanguination 54 39.68 Quite Low
Disease 18 29.72 Quite Low
Severe Combined Immunodeficiency 36 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
FACS (Fluorescence-Activated Cell Sorter) analysis revealed that almost all PBL and BMC showed membrane expression of the hPLAP enzyme in hPLAP-tg rats (Fig. 2A and 2B).
Gene_expression (expression) of hPLAP in PBL
1) Confidence 0.20 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.54 Pain Relevance 0
hPLAP-tg rats express hPLAP mainly in the cell membrane of most cell types as demonstrated by FACS analysis in our study.
Gene_expression (express) of hPLAP
2) Confidence 0.20 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.18 Pain Relevance 0
Fig. 3A–C shows that in irradiated wild-type rats transplanted with hPLAP-tg bone marrow [WT (hPLAP-BMT)] all hematopoietic cells are of donor origin, and stably express hPLAP, 4 weeks post-transplantation.
Gene_expression (express) of hPLAP in hematopoietic cells
3) Confidence 0.20 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.24 Pain Relevance 0.06
This finding prompted us to ask the question whether expression of the transgene, and especially membrane expression of hPLAP, would induce the formation of antibodies to hPLAP in the wild-type recipients.
Gene_expression (expression) of hPLAP
4) Confidence 0.20 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.52 Pain Relevance 0
hPLAP-tg rats express hPLAP mainly in the cell membrane of most cell types as demonstrated by FACS analysis in our study.
Gene_expression (express) of hPLAP
5) Confidence 0.20 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.18 Pain Relevance 0
Immunization phenomena against hPLAP could result in immune-mediated destruction of any hPLAP-expressing cells in wild-type recipients, because the hPLAP enzyme represents a foreign protein to the rat immune system.
Gene_expression (destruction) of hPLAP in immune system associated with immunization
6) Confidence 0.18 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.59 Pain Relevance 0
FACS (Fluorescence-Activated Cell Sorter) analysis revealed that almost all PBL and BMC showed membrane expression of the hPLAP enzyme in hPLAP-tg rats (Fig. 2A and 2B).
Gene_expression (expression) of hPLAP in PBL
7) Confidence 0.18 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.54 Pain Relevance 0
The superb histological detection quality of hPLAP permits a clear distinction between wild-type cells of recipient origin and hPLAP-expressing cells of donor origin (Fig. 3D–F).
Gene_expression (expressing) of hPLAP
8) Confidence 0.16 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.27 Pain Relevance 0.03
We used inbred transgenic Fischer 344 rats expressing human placental alkaline phosphatase (hPLAP) under the control of the ubiquitous R26 promoter for this study. hPLAP is an excellent marker enzyme, providing superb histological detection quality in paraffin and plastic sections.


Gene_expression (expressing) of hPLAP associated with targeted disruption
9) Confidence 0.16 Published 2007 Journal BMC Biotechnol Section Abstract Doc Link PMC1899491 Disease Relevance 0.18 Pain Relevance 0.03
Transgenic mice and rats expressing hPLAP under the control of the R26 promoter show ubiquitous, uniform, and stable expression of this genetic marker [4].
Gene_expression (expressing) of hPLAP associated with targeted disruption
10) Confidence 0.16 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.27 Pain Relevance 0
However, histological analysis of lung, liver, spleen, lymph nodes, and bone failed to show any evidence of hPLAP-expressing cells from transgenic donors in the wild-type recipients, suggesting that it was not possible to syngeneically transplant cells from transgenic donors into wild-type rats (Fig. 1).
Gene_expression (expressing) of hPLAP in liver associated with targeted disruption
11) Confidence 0.16 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.42 Pain Relevance 0
These data clearly demonstrate that sustainable marker tolerance also can be achieved by neonatal exposure of wild-type F344 rats to hPLAP-expressing cells of the same rat strain.
Gene_expression (expressing) of hPLAP associated with tolerance and sprains and strains
12) Confidence 0.16 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.74 Pain Relevance 0.08
However, cell proliferation was induced when spleen cells from wild-type rats that had previously been exposed to hPLAP-expressing cells were co-cultured with transgenic cells.
Gene_expression (expressing) of hPLAP in spleen associated with targeted disruption
13) Confidence 0.16 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.83 Pain Relevance 0.03
To examine the presence of circulating anti-hPLAP antibodies in serum of wild-type rats that had previously received cells from hPLAP-tg rats, wild-type and transgenic peripheral blood cells were incubated for 30 min on ice with 10 ?
Gene_expression (antibodies) of hPLAP in blood cells associated with targeted disruption
14) Confidence 0.15 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.24 Pain Relevance 0
Fig. 3A–C shows that in irradiated wild-type rats transplanted with hPLAP-tg bone marrow [WT (hPLAP-BMT)] all hematopoietic cells are of donor origin, and stably express hPLAP, 4 weeks post-transplantation.
Gene_expression (transplanted) of hPLAP in hematopoietic cells
15) Confidence 0.15 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.25 Pain Relevance 0.06
However, histological analysis of lung, liver, spleen, lymph nodes, and bone failed to show any evidence of hPLAP-expressing cells from transgenic donors in the wild-type recipients, suggesting that it was not possible to syngeneically transplant cells from transgenic donors into wild-type rats (Fig. 1).
Gene_expression (expressing) of hPLAP in lymph nodes associated with targeted disruption
16) Confidence 0.05 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.42 Pain Relevance 0
However, histological analysis of lung, liver, spleen, lymph nodes, and bone failed to show any evidence of hPLAP-expressing cells from transgenic donors in the wild-type recipients, suggesting that it was not possible to syngeneically transplant cells from transgenic donors into wild-type rats (Fig. 1).
Gene_expression (expressing) of hPLAP in lung associated with targeted disruption
17) Confidence 0.05 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.42 Pain Relevance 0
However, histological analysis of lung, liver, spleen, lymph nodes, and bone failed to show any evidence of hPLAP-expressing cells from transgenic donors in the wild-type recipients, suggesting that it was not possible to syngeneically transplant cells from transgenic donors into wild-type rats (Fig. 1).
Gene_expression (expressing) of hPLAP in spleen associated with targeted disruption
18) Confidence 0.05 Published 2007 Journal BMC Biotechnol Section Body Doc Link PMC1899491 Disease Relevance 0.42 Pain Relevance 0

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