INT211584

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Context Info
Confidence 0.73
First Reported 2007
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 32
Total Number 46
Disease Relevance 12.28
Pain Relevance 0.46

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytosol (Dmpk) mitochondrion (Dmpk) endoplasmic reticulum (Dmpk)
plasma membrane (Dmpk) nucleus (Dmpk) kinase activity (Dmpk)
Anatomy Link Frequency
myoblasts 8
skeletal muscle 2
muscle 1
brain 1
nucleus 1
Dmpk (Mus musculus)
Pain Link Frequency Relevance Heat
cytokine 30 90.12 High High
Inflammation 15 82.08 Quite High
tolerance 64 68.64 Quite High
anesthesia 35 65.60 Quite High
abdominal pain 13 37.40 Quite Low
fibrosis 17 17.96 Low Low
Central nervous system 30 5.00 Very Low Very Low Very Low
Kinase C 26 5.00 Very Low Very Low Very Low
spastic colon 15 5.00 Very Low Very Low Very Low
depression 15 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Targeted Disruption 700 100.00 Very High Very High Very High
Myotonic Dystrophy 434 100.00 Very High Very High Very High
Toxicity 436 99.32 Very High Very High Very High
Hypertrophy 49 99.08 Very High Very High Very High
Muscular Atrophy 60 98.16 Very High Very High Very High
Myotonia 210 96.92 Very High Very High Very High
Hypopituitarism 161 96.52 Very High Very High Very High
Arrhythmias 2 Under Development 180 96.20 Very High Very High Very High
Arrhythmia Under Development 73 96.12 Very High Very High Very High
Polyploidy 5 95.80 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Expansion appears also transcription independent, as the Dmpk gene is expressed at very low levels in hepatocytes and appears fully repressed in acinar cells [[21], own findings, unpublished].
Gene_expression (expressed) of Dmpk gene in acinar cells
1) Confidence 0.73 Published 2007 Journal BMC Mol Biol Section Body Doc Link PMC1940261 Disease Relevance 0.25 Pain Relevance 0
In contrast, expression of a high number of copies of the DMPK 3’ UTR containing 5 CUG repeats leads to development of DM1-like phenotype (myotonia, cardiac conduction defects and muscular dystrophy) in the absence of nuclear CUG foci and in the absence of MBNL1 sequestration [34].
Gene_expression (expression) of DMPK associated with myotonia, frailty and arrhythmias 2 under development
2) Confidence 0.68 Published 2010 Journal Current Genomics Section Body Doc Link PMC2874224 Disease Relevance 0.78 Pain Relevance 0
Real-time PCR was carried out to quantitate the expression levels of LacZ-(CUG)400 and DMPK 11-15(CUG)300 transcripts.
Gene_expression (expression) of DMPK
3) Confidence 0.68 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2597774 Disease Relevance 0 Pain Relevance 0
Moreover, overexpression of DMPK 3' UTR mRNA in mouse skeletal muscle also induced transcriptional activation of Nkx2-5 and its targets.
Gene_expression (overexpression) of DMPK in skeletal muscle
4) Confidence 0.63 Published 2008 Journal Current Genomics Section Body Doc Link PMC2694559 Disease Relevance 0.42 Pain Relevance 0
The possibility that CTG and CCTG repeat expansions were altering the local structure of the chromatin (a cis-effect; Fig. 1), thus interfering with the expression of DMPK and neighbouring genes, has also been considered.
Gene_expression (expression) of DMPK
5) Confidence 0.63 Published 2008 Journal Current Genomics Section Body Doc Link PMC2694559 Disease Relevance 0.57 Pain Relevance 0.03
Overexpression of a normal DMPK 3´UTR (five CUG repeats) as part of an inducible RNA transcript was reported to cause myotonia, cardiac conduction abnormalities, histopathology and RNA splicing defects in the absence of detectable nuclear inclusions.
Gene_expression (Overexpression) of DMPK associated with arrhythmia under development and hypopituitarism
6) Confidence 0.63 Published 2008 Journal Current Genomics Section Body Doc Link PMC2694559 Disease Relevance 0.46 Pain Relevance 0
On the one hand, the activity of the ribonuclease downregulates the mutant DMPK transcript, which removes a toxic RNA from the cell and may contribute to the reduction of DMPK expression levels.
Gene_expression (expression) of DMPK
7) Confidence 0.63 Published 2008 Journal Current Genomics Section Body Doc Link PMC2694559 Disease Relevance 0 Pain Relevance 0
A requirement for general transcription factors in different tissues that express DMPK would account for the multisystemic and multisymptomatic nature of DM1.
Gene_expression (express) of DMPK
8) Confidence 0.63 Published 2008 Journal Current Genomics Section Body Doc Link PMC2694559 Disease Relevance 0.28 Pain Relevance 0
It has been recently found that, in DM1 cells, the mutant DMPK mRNA is detected in aggregated forms in both nucleus and in cytoplasm [47].
Gene_expression (detected) of mutant DMPK mRNA in nucleus
9) Confidence 0.59 Published 2010 Journal Current Genomics Section Body Doc Link PMC2874224 Disease Relevance 0 Pain Relevance 0
The initial studies showed that the sensitivity of FISH assay is very important because cytoplasmic mutant DMPK mRNA is detected in the multiple complexes of smaller size relatively to very large nuclear foci [26].
Gene_expression (detected) of mutant DMPK mRNA
10) Confidence 0.59 Published 2010 Journal Current Genomics Section Body Doc Link PMC2874224 Disease Relevance 0 Pain Relevance 0
In contrast to the nuclear foci, the cytoplasmic mutant DMPK mRNA was detected in small complexes [26].
Gene_expression (detected) of mutant DMPK mRNA
11) Confidence 0.59 Published 2010 Journal Current Genomics Section Body Doc Link PMC2874224 Disease Relevance 0 Pain Relevance 0
Consistent with these findings, expression of the mutant DMPK in C2C12 myoblasts increases stability of TNF mRNA [87].
Gene_expression (expression) of DMPK in myoblasts
12) Confidence 0.59 Published 2010 Journal Current Genomics Section Body Doc Link PMC2874224 Disease Relevance 0.39 Pain Relevance 0.12
To further confirm that comparable expression levels of LacZ-(CUG)400, and DMPK 11-15(CUG)300 RNAs were assayed in these experiments, we quantitated the amounts of LacZ-(CTG)400, and DMPK 11-15(CTG)300 cDNAs by Real-time PCR analyses.
Gene_expression (expression) of DMPK
13) Confidence 0.59 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2597774 Disease Relevance 0.10 Pain Relevance 0
Use of a standard curve generated from plasmid DNAs encoding these sequences demonstrates that the steady-state expression levels of the LacZ-(CTG)400, and DMPK 11-15(CTG)300 cDNAs are similar [LacZ-(CTG)400?
Gene_expression (expression) of DMPK
14) Confidence 0.59 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2597774 Disease Relevance 0.07 Pain Relevance 0
These data therefore demonstrate that when expanded CUG tracts, expressed in a context independent manner or in the context of the DMPK 3?
Gene_expression (context) of DMPK
15) Confidence 0.59 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2597774 Disease Relevance 0.12 Pain Relevance 0.05
As noted above, a study by Wang and colleagues demonstrates that inducible expression of 960 interrupted CUG repeats located in the DMPK 3?
Gene_expression (expression) of DMPK
16) Confidence 0.59 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2597774 Disease Relevance 0.28 Pain Relevance 0
Expression of LacZ-(CUG)400 RNAs at levels equivalent to that of a DMPK mini gene encoding 300 CTG repeats does not result in aberrant RNA splicing in human myoblasts
Gene_expression (Expression) of DMPK mini gene in myoblasts
17) Confidence 0.59 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2597774 Disease Relevance 0.24 Pain Relevance 0
Consistent with our study, previous experiments in mouse myoblast cultures demonstrate that CTG tracts expressed in the context of the DMPK 3?
Gene_expression (context) of DMPK in myoblast
18) Confidence 0.59 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2597774 Disease Relevance 0.15 Pain Relevance 0.07
Thus when expressed at a comparable level to the DMPK minigene encoding 300 CTG repeats [DMPK 11-15(CUG)300], the LacZ-(CUG)400 RNAs are unable to dysregulate splicing in human myoblasts.


Gene_expression (expressed) of DMPK minigene in myoblasts
19) Confidence 0.59 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2597774 Disease Relevance 0.09 Pain Relevance 0
Expression of constructs encoding both the DMPK 11-15 minigene and GFP-DMPK 3?
Gene_expression (Expression) of DMPK
20) Confidence 0.59 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2597774 Disease Relevance 0.25 Pain Relevance 0

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