INT215261
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Presently the molecular mechanisms by which SN leads to the inhibition of p47phox translocation are under investigation. | |||||||||||||||
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Therefore, one mechanism by which SN inhibits superoxide production in microglial cells is through inhibition of p47phox translocation to the cell membrane following LPS stimulation.
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In summary, this study is the first report indicating that SN is a potent anti-inflammatory and neuroprotective agent that acts through inhibition of microglial PHOX-generated superoxide by inhibiting the translocation of p47phox to the plasma membrane. | |||||||||||||||
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However, the addition of 10-14 M SN significantly prevented this LPS-induced p47phox translocation (Fig. 5, panel V and VI). | |||||||||||||||
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We found that the translocation of the p47phox subunit from the cytosol to the plasma membrane following LPS stimulation was inhibited by SN. | |||||||||||||||
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A critical role for PHOX in FLZ-elicited neuroprotection was further supported by the findings that 1) FLZ's protective effect was reduced in cultures from PHOX-/- mice, and 2) FLZ inhibited LPS-induced translocation of the cytosolic subunit of p47PHOX to the membrane and thus inhibited the activation of PHOX. | |||||||||||||||
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Western blot analysis from this study revealed that FLZ inhibited LPS-induced p47phox translocation from cytosol to membrane. | |||||||||||||||
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Thus, this result further proves that FLZ-mediated inhibition of superoxide production by LPS is mediated through suppression of p47phox membrane translocation; that is, activation of PHOX.
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