INT215979

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Context Info
Confidence 0.34
First Reported 2007
Last Reported 2008
Negated 0
Speculated 1
Reported most in Body
Documents 4
Total Number 12
Disease Relevance 0.77
Pain Relevance 0.26

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytoskeletal protein binding (Gphn) plasma membrane (Gphn) cytoskeleton (Gphn)
intracellular (Gphn) cytoplasm (Gphn)
Anatomy Link Frequency
neurons 1
synapses 1
Gphn (Mus musculus)
Pain Link Frequency Relevance Heat
depression 16 89.72 High High
amygdala 2 83.92 Quite High
GABAergic 58 83.68 Quite High
Hippocampus 21 82.76 Quite High
Spinal cord 27 75.52 Quite High
imagery 22 51.32 Quite High
Glutamate receptor 30 39.36 Quite Low
tetrodotoxin 10 27.60 Quite Low
agonist 11 19.28 Low Low
gABA 57 16.84 Low Low
Disease Link Frequency Relevance Heat
Anxiety Disorder 8 96.44 Very High Very High Very High
Depression 16 89.72 High High
Targeted Disruption 77 84.68 Quite High
Death 9 60.24 Quite High
Neurologic Manifestations 9 40.32 Quite Low
Disease 11 26.48 Quite Low
Sprains And Strains 27 17.04 Low Low
Generalized Anxiety Disorder 4 5.00 Very Low Very Low Very Low
Stress 4 5.00 Very Low Very Low Very Low
Epilepsy 4 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
As shown in Fig. 2c, scFvGeph-2/scFvGeph-9 antibodies were able to co-immunoprecipitate gephyrin, suggesting that they interacted with gephyrin intracellularly.
gephyrin Binding (interacted) of
1) Confidence 0.34 Published 2007 Journal J Mol Neurosci Section Body Doc Link PMC2758390 Disease Relevance 0 Pain Relevance 0
Figure 2Gephyrin-specific intrabodies interact with gephyrin in mammalian cells.
gephyrin Binding (interact) of
2) Confidence 0.34 Published 2007 Journal J Mol Neurosci Section Body Doc Link PMC2758390 Disease Relevance 0 Pain Relevance 0
This domain was chosen as it is not engaged in gephyrin oligomerization, a process regarded as the molecular mechanism responsible for inhibitory receptors accumulation at postsynaptic sites (Sola et al. 2004).
gephyrin Binding (oligomerization) of
3) Confidence 0.34 Published 2007 Journal J Mol Neurosci Section Body Doc Link PMC2758390 Disease Relevance 0.16 Pain Relevance 0
The ability of the selected intrabodies to recognize gephyrin in mammalian cells was assessed in immunocytochemical experiments performed on HEK 293 cells co-transfected with scFvGeph-2/scFvGeph-9 and gephyrin fused to EGFP.
gephyrin Binding (recognize) of
4) Confidence 0.34 Published 2007 Journal J Mol Neurosci Section Body Doc Link PMC2758390 Disease Relevance 0 Pain Relevance 0
As a consequence, the selected intrabodies should recognize gephyrin upon its self-assembly into the submembraneous protein lattice underlying inhibitory receptor clusters.
gephyrin Binding (recognize) of
5) Confidence 0.34 Published 2007 Journal J Mol Neurosci Section Body Doc Link PMC2758390 Disease Relevance 0.15 Pain Relevance 0
Receptor anchoring is based on the interaction of gephyrin with the actin- and microtubule-based cytoskeleton (Kirsch 2006).
gephyrin Binding (interaction) of
6) Confidence 0.30 Published 2007 Journal J Mol Neurosci Section Body Doc Link PMC2758390 Disease Relevance 0.19 Pain Relevance 0.04
Gephyrin binds with high affinity to the ?
Gephyrin Binding (binds) of
7) Confidence 0.29 Published 2007 Journal J Mol Neurosci Section Body Doc Link PMC2758390 Disease Relevance 0 Pain Relevance 0.03
The ability of the anti-gephyrin intrabodies to bind and remove endogenous gephyrin from glycine receptor clusters was functionally assessed on cultured hippocampal neurons, known to highly express GlyRs (Danglot et al. 2004; Ito and Cherubini 1991).
anti-gephyrin Binding (bind) of in neurons
8) Confidence 0.26 Published 2007 Journal J Mol Neurosci Section Body Doc Link PMC2758390 Disease Relevance 0 Pain Relevance 0
Immunoprecipitates were analyzed by western blotting using anti-FLAG and anti-SV5 antibodies, as indicated

Gephyrin-Specific Intrabodies Alter Endogenous Glycine Receptor Function

Gephyrin Spec (analyzed) Binding (indicated) of
9) Confidence 0.26 Published 2007 Journal J Mol Neurosci Section Body Doc Link PMC2758390 Disease Relevance 0 Pain Relevance 0
An indirect interaction of gephyrin with the actin cytoskeleton is mediated by the actin binding proteins profilin I and IIA and by the microfilament adaptors of the Mena/VASP family that also interact with each other and regulate microfilament dynamics (Lambrechts et al., 2000).
gephyrin Binding (interaction) of
10) Confidence 0.09 Published 2008 Journal Frontiers in Molecular Neuroscience Section Body Doc Link PMC2526003 Disease Relevance 0.26 Pain Relevance 0.13
The role of gephyrin has been extensively investigated at glycinergic synapses, as its strong direct interaction with the glycine receptor has long been established.
gephyrin Binding (interaction) of in synapses
11) Confidence 0.08 Published 2008 Journal Frontiers in Molecular Neuroscience Section Body Doc Link PMC2526003 Disease Relevance 0 Pain Relevance 0.07
Of course, we cannot exclude subtle differences between somatic and extrasynaptic receptors, because of binding to the cytoskeleton through gephyrin or differences in intracellular modulation, for instance by phosphorylation (reviewed by Moss & Smart, 2001).
gephyrin Binding (binding) of
12) Confidence 0.08 Published 2007 Journal The Journal of Physiology Section Body Doc Link PMC2075563 Disease Relevance 0 Pain Relevance 0

General Comments

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