INT216751

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.60
First Reported 2006
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 36
Total Number 37
Disease Relevance 3.42
Pain Relevance 15.60

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

plasma membrane (ADORA2B) signal transducer activity (ADORA2B)
Anatomy Link Frequency
NG108-15 2
neuronal 1
CPA 1
pancreatic beta cell 1
embryonic kidney 1
ADORA2B (Homo sapiens)
Pain Link Frequency Relevance Heat
adenocard 4588 100.00 Very High Very High Very High
antagonist 1561 99.96 Very High Very High Very High
agonist 1056 98.42 Very High Very High Very High
analgesia 4 98.32 Very High Very High Very High
Opioid 6 97.92 Very High Very High Very High
Analgesic 35 92.08 High High
Inflammation 20 88.40 High High
chemokine 4 80.76 Quite High
cytokine 4 80.20 Quite High
tolerance 31 64.76 Quite High
Disease Link Frequency Relevance Heat
Neuroblastoma 124 99.04 Very High Very High Very High
Glioma 124 99.00 Very High Very High Very High
Hypoglycemia 31 96.32 Very High Very High Very High
Nociception 8 94.48 High High
Diabetes Mellitus 155 90.24 High High
Asthma 51 88.72 High High
INFLAMMATION 51 88.40 High High
Airway Remodeling 4 88.16 High High
Diabetic Retinopathy 39 70.88 Quite High
Breast Cancer 4 65.36 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The non-specific binding amounted to 20–30% of total binding at a radioligand concentration of 1 nM used in competition assays in membranes expressing an A2B receptor density of 1.6 pmol per milligram of protein using 50 µg of protein in 200 ml TRIS buffer (50 mM, pH 7.4) containing no additives.
Gene_expression (expressing) of A2B receptor
1) Confidence 0.60 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0 Pain Relevance 0.41
The high expression of A2B receptors in different parts of the intestinal tract raised great interest in defining their function.
Gene_expression (expression) of A2B
2) Confidence 0.58 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096665 Disease Relevance 0.61 Pain Relevance 0.47
In spite of the high affinity and selectivity, this radioligand has not been used in cells and tissues endogenously expressing A2B receptors.
Gene_expression (expressing) of A2B
3) Confidence 0.58 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096665 Disease Relevance 0 Pain Relevance 0.24
Using membranes from HEK 293 cells expressing the human recombinant A2B receptor, [3H]OSIP339391 was characterized in kinetic, saturation and competition binding experiments.
Gene_expression (expressing) of A2B receptor
4) Confidence 0.58 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096665 Disease Relevance 0 Pain Relevance 0.24
The adenosine receptors A1 (ADORA1), A2A (ADORA2A), A2B (ADORA2B), and A3 (ADORA3) group together, indicating overlap in ligand profiles.
Gene_expression (group) of ADORA2B associated with adenocard
5) Confidence 0.54 Published 2010 Journal BMC Bioinformatics Section Body Doc Link PMC2897831 Disease Relevance 0 Pain Relevance 0.23
The adenosine receptors A1 (ADORA1), A2A (ADORA2A), A2B (ADORA2B), and A3 (ADORA3) group together, indicating overlap in ligand profiles.
Gene_expression (group) of A2B associated with adenocard
6) Confidence 0.54 Published 2010 Journal BMC Bioinformatics Section Body Doc Link PMC2897831 Disease Relevance 0 Pain Relevance 0.23
Thus, we used the radioligand for characterizing adenosine A2B receptors expressed on the rat pancreatic betacell line INS-1.
Gene_expression (expressed) of adenosine A2B associated with adenocard
7) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0.23 Pain Relevance 0.84
Figure 9Competition experiments of the antagonists caffeine, theophylline, alloxazine and CGS-15943 (a) and the agonists CADO, CPA and NECA (b) versus 1 nM [3H]PSB-298 performed at human recombinant adenosine A2B receptors expressed in HEK-293 cells
Gene_expression (expressed) of adenosine A2B in CPA associated with adenocard, antagonist and agonist
8) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0 Pain Relevance 0.54
Commercially available membranes from HEK-293 cells expressing the human adenosine A2B receptor in a high density (Bmax value of 1.6 pmol per milligram protein) were used to evaluate the new antagonist radioligand.
Gene_expression (expressing) of adenosine A2B receptor associated with adenocard and antagonist
9) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0 Pain Relevance 0.15
Mundell and Kelly had shown in functional assays that the mouse neuroblastoma x rat glioma hybrid cell line NG108-15 developed by Hamprecht [34], which is frequently used as a model for neuronal cells, expressed two different adenosine receptor subtypes, A2A and A2B [20].
Gene_expression (expressed) of A2B in neuronal associated with neuroblastoma, adenocard and glioma
10) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0.20 Pain Relevance 0.53
The mouse neuroblastoma x rat glioma hybrid cell line had previously been proposed to express adenosine A2A as well as adenosine A2B receptors on the basis of functional assays [20].
Gene_expression (express) of adenosine A2B associated with neuroblastoma, adenocard and glioma
11) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0.20 Pain Relevance 0.42
These experiments suggest that the expression level of adenosine A2B receptors is much higher than that of adenosine A2A receptors in NG108-15 cells.
Gene_expression (expression) of adenosine A2B in NG108-15 associated with adenocard
12) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0.13 Pain Relevance 0.65
Figure 5Kinetics of [3H]PSB-298 binding (1 nM) to membranes recombinantly expressing the human adenosine A2B receptor at 25°C.
Gene_expression (expressing) of adenosine A2B receptor associated with adenocard
13) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0 Pain Relevance 0.19
The use of [3H]PSB-298 allowed the direct evidence of human adenosine A2B receptors expressed in CHO cells that had been transfected and functionally characterized by Klotz et al. [18].
Gene_expression (expressed) of adenosine A2B associated with adenocard
14) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0.12 Pain Relevance 0.57
Based on these results, the new radioligand [3H]PSB-298 was subsequently used for characterizing two cell lines believed to be natively expressing adenosine A2B receptors.


Gene_expression (expressing) of adenosine A2B associated with adenocard
15) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0.19 Pain Relevance 0.41
The rat pancreatic beta cell line INS-1 was investigated with respect to adenosine A2B receptor expression using [3H]PSB-298.
Gene_expression (expression) of adenosine A2B receptor in pancreatic beta cell associated with adenocard
16) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0.18 Pain Relevance 0.54
Saturation experiments demonstrated that [3H]PSB-298 bound to a single class of binding sites in the HEK-293 cell membranes recombinantly expressing the human adenosine A2B receptor with a KD value of 60 ± 1 nM and an apparent Bmax value of 3,511 ± 93 fmol per milligram protein (Figure 6).
Gene_expression (expressing) of adenosine A2B receptor associated with adenocard
17) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0 Pain Relevance 0.24
HEK-293 cell membranes recombinantly expressing the human adenosine A2B receptor (Bmax = 1.6 pmol per milligram protein detected with [3H]DPCPX) were purchased from Perkin Elmer Life Sciences, Boston, USA.
Gene_expression (expressing) of adenosine A2B receptor associated with adenocard
18) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0 Pain Relevance 0.09
Subsequently, three different cell lines were characterized using [3H]PSB-298 as an antagonist radioligand for adenosine A2B receptors: a frequently used recombinant CHO cell line expressing the human adenosine A2B receptor, a native rodent mouse neuroblastoma x rat glioma hybrid cell line (NG-108-15) and the rat insulinoma cell line INS-1.
Gene_expression (expressing) of adenosine A2B receptor associated with hypoglycemia, neuroblastoma, adenocard, antagonist and glioma
19) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0.29 Pain Relevance 0.65
Membranes from HEK-293 cells expressing human adenosine A2B receptors in a high density were used to evaluate the new antagonist radioligand and to test its suitability for radioligand binding studies at adenosine A2B receptors.
Gene_expression (expressing) of adenosine A2B associated with adenocard and antagonist
20) Confidence 0.52 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096648 Disease Relevance 0.19 Pain Relevance 0.71

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox