INT217501

Context Info Confidence 0.49 First Reported 2007 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 8 Total Number 9 Disease Relevance 2.91 Pain Relevance 0.28

Golgi apparatus (Tjp1) plasma membrane (Tjp1) nucleus (Tjp1) cytoplasm (Tjp1)

Anatomy Link Frequency IECs 2 Tjp1 (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 60 92.68 Crohn's disease 24 57.68 cytokine 12 6.48 Versed 12 5.00 anesthesia 9 5.00 Central nervous system 6 5.00 tetrodotoxin 6 5.00 imagery 4 5.00 Inflammatory mediators 2 5.00 Neurobehavioral 2 5.00

Disease Link Frequency Relevance Heat Colitis 186 99.46 Congenital Anomalies 2 98.96 INFLAMMATION 56 92.68 Body Weight 46 91.92 Diabetic Angiopathy 1 90.32 Sprains And Strains 12 85.08 Injury 6 84.64 Inflammatory Bowel Disease 102 75.20 Weight Loss 12 73.88 Infection 26 73.68

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

This hypothesis is consistent with recently published data regarding the antrum mucosal protein (AMP)-18 that ameliorates DSS colitis in mice and also enhances accumulation of occludin and ZO-1 in TJ domains in vitro [55].

Positive_regulation (accumulation) of ZO-1 associated with colitis

1) Confidence 0.49 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.66 Pain Relevance 0.03

IECs of mice treated with DSS and EcN showed elevated ZO-1 mRNA levels in comparison to DSS treated animals (p<0.05) (Figure 5).

Positive_regulation (elevated) of ZO-1 mRNA

2) Confidence 0.49 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.52 Pain Relevance 0.10

Comparison of ZO-1 staining along the surface of the crypts revealed only a slight increase of ZO-1 protein in IECs of mice colonized with EcN.

Positive_regulation (increase) of ZO-1 protein

3) Confidence 0.49 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0 Pain Relevance 0

As depicted in figure 2B colonization of gnotobiotic mice with EcN resulted in a specific up-regulation of ZO-1 mRNA in IECs (p<0.05).

Positive_regulation (regulation) of ZO-1 mRNA

4) Confidence 0.33 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0 Pain Relevance 0

In order to study functional consequences of DSS-mediated colitis and EcN treatment and also the potential significance of ZO-1 up-regulation for an enhancement of intestinal barrier function, we performed experiments aimed to assess transport and barrier function of the colonic epithelium of DSS-treated mice with and without application of EcN.

Positive_regulation (regulation) of ZO-1 in epithelium associated with colitis

5) Confidence 0.33 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.71 Pain Relevance 0.07

Colonization of gnotobiotic mice with EcN resulted in an up-regulation of ZO-1 in IECs at both mRNA and protein levels.

Positive_regulation (regulation) of ZO-1 in IECs

6) Confidence 0.33 Published 2007 Journal PLoS ONE Section Abstract Doc Link PMC2110898 Disease Relevance 0.67 Pain Relevance 0.08

The expression level of ZO-1 was increased after treatment with PCB118 or PCB153, without any changes in PCB126-exposed animals.

Positive_regulation (increased) of ZO-1

7) Confidence 0.12 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2854723 Disease Relevance 0 Pain Relevance 0

Results of fluorescein angiography, Evans blue extravasation, and western blotting for association of the tight junction proteins occludin and zo-1 suggest that endogenous TGF-?

Positive_regulation (Results) of zo-1

8) Confidence 0.11 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2659748 Disease Relevance 0.37 Pain Relevance 0

Compared with occludin, the immunostaining results for ZO-1 suggest a qualitative increase in immunoreactivity after treatment with individual PCB congeners (Figure 3).

Positive_regulation (results) of ZO-1

9) Confidence 0.09 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2854723 Disease Relevance 0 Pain Relevance 0

General Comments

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