INT217504

Context Info Confidence 0.32 First Reported 2007 Last Reported 2010 Negated 2 Speculated 2 Reported most in Body Documents 9 Total Number 10 Disease Relevance 2.44 Pain Relevance 0.23

Golgi apparatus (Tjp1) plasma membrane (Tjp1) nucleus (Tjp1) cytoplasm (Tjp1)

Anatomy Link Frequency retinas 1 IECs 1 gut 1 Tjp1 (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 40 92.68 agonist 3 92.00 Crohn's disease 16 73.52 metalloproteinase 3 70.40 Inflammatory mediators 3 51.28 imagery 19 32.40 Central nervous system 10 31.68 Kinase C 1 27.52 Neurobehavioral 3 15.08 anesthesia 10 5.00

Disease Link Frequency Relevance Heat Coronary Artery Disease 6 99.36 Colitis 124 99.00 Stress 9 93.28 INFLAMMATION 39 92.68 Body Weight 35 91.92 Weight Loss 8 89.72 Sprains And Strains 9 85.08 Inflammatory Bowel Disease 68 75.20 Infection 22 73.68 Disease 18 73.52

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Colonization of gnotobiotic mice with EcN resulted in an up-regulation of ZO-1 in IECs at both mRNA and protein levels.

Regulation (regulation) of ZO-1 in IECs

1) Confidence 0.32 Published 2007 Journal PLoS ONE Section Abstract Doc Link PMC2110898 Disease Relevance 0.67 Pain Relevance 0.08

As depicted in figure 2B colonization of gnotobiotic mice with EcN resulted in a specific up-regulation of ZO-1 mRNA in IECs (p<0.05).

Regulation (regulation) of ZO-1 mRNA

2) Confidence 0.32 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0 Pain Relevance 0

Due to the important functional role of ZO-1 in the junctional complex, these findings suggest that enhancement of the intestinal epithelial barrier function by EcN could at least in part be attributed to up-regulation of ZO-1.

Regulation (regulation) of ZO-1

3) Confidence 0.32 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.12 Pain Relevance 0

This indicates a considerable association between the severity of colitis, as evidenced by increased gut permeability, and altered ZO-1 mRNA expression levels.

Regulation (altered) of ZO-1 mRNA in gut associated with colitis

4) Confidence 0.19 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.62 Pain Relevance 0.04

The Ras GTPase pathway plays an important role in the regulation of claudin-5, ZO-1, and ZO-2 (Zhong et al. 2008), and the Rho pathway is critical for TJ assembly (Persidsky et al. 2006).

Regulation (regulation) of ZO-1

5) Confidence 0.11 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2854723 Disease Relevance 0.33 Pain Relevance 0.04

-cat, Plako, ZO-1, Ocln, Cldn1, and Cldn12 were not modified in distribution and expression (Fig. 3 and not depicted).

Neg (not) Regulation (modified) of ZO-1

6) Confidence 0.09 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2575783 Disease Relevance 0.16 Pain Relevance 0

While there was no change in the levels of the tight junction proteins zo-1 and occludin in retinas from sEng-expressing mice (data not shown), there was a decrease in association between these proteins, a characteristic feature of tight junction disassembly [28].

Neg (no) Regulation (change) of zo-1 in retinas

7) Confidence 0.09 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2659748 Disease Relevance 0.07 Pain Relevance 0

Therefore, we examined the effects of PCB treatment on the localization profiles of claudin-5, occludin, and ZO-1 in freshly isolated microvessels (Figures 2 and 3).

Spec (examined) Regulation (effects) of ZO-1

8) Confidence 0.08 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2854723 Disease Relevance 0 Pain Relevance 0

Exposure to PCB118 and PCB153 modulated expressional levels of claudin-5 and ZO-1, whereas PCB126 appeared to primarily influence the coassociation of occludin and ZO-1.

Regulation (modulated) of ZO-1

9) Confidence 0.05 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2854723 Disease Relevance 0.22 Pain Relevance 0.07

To determine whether the molecular alterations of EMT occurred in these cells, we examined the localization of several adherent junction proteins, such as E-cadherin and ZO-1, by confocal immunofluorescence staining.

Spec (examined) Regulation (localization) of ZO-1

10) Confidence 0.04 Published 2010 Journal BMC Cancer Section Body Doc Link PMC2912267 Disease Relevance 0.24 Pain Relevance 0

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