INT220472

Context Info Confidence 0.11 First Reported 2007 Last Reported 2007 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 22 Disease Relevance 13.93 Pain Relevance 0.12

cell differentiation (BAG6) cytosol (BAG6) transport (BAG6) nucleus (BAG6) embryo development (BAG6) cytoplasm (BAG6)

Anatomy Link Frequency endothelial cells 6 spine 1 BAG6 (Homo sapiens)

Pain Link Frequency Relevance Heat Sciatic nerve 22 88.60 imagery 22 83.60 Inflammation 22 60.12 anesthesia 22 46.20

Disease Link Frequency Relevance Heat Breast Cancer 770 99.84 Metastasis 572 99.84 Cancer 1672 99.60 Weight Loss 44 99.50 Injury 110 98.40 Prostate Cancer 22 97.56 Osteolysis 308 97.12 Heart Neoplasms 22 95.84 Apoptosis 110 95.58 Carcinoma 22 95.52

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Anatomy

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Speculation

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Human MT-1 cells expressing versican G3 or vector control constructs were cultured at a density of 1.2 × 106 cells/ml in 175 cm2 flasks in RPMI-1640 media (Life Technologies, Inc.

Gene_expression (expressing) of G3

1) Confidence 0.11 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.46 Pain Relevance 0

The G3 construct and a control vector were stably transfected into human breast carcinoma MT-1 cells using Lipofectamine 2000.

Gene_expression (transfected) of G3 associated with breast cancer

2) Confidence 0.11 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.54 Pain Relevance 0

This was consistent with the observation of increased versican G3 expression as assessed by immunoblotting in the experimental group following tumor tissue harvest when compared to the vector control group (Figure 2c).

Gene_expression (expression) of G3 associated with cancer

3) Confidence 0.11 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.41 Pain Relevance 0

G3 mediated effects on endothelial cells

Gene_expression (effects) of G3 in endothelial cells

4) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.38 Pain Relevance 0

In vivo G3 effects on metastasis

Gene_expression (effects) of G3 associated with metastasis

5) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.44 Pain Relevance 0

Concordantly, a greater tumor burden as reflected by histologic staining for hEGFr was observed in harvested lumbar spine of versican G3 rats.

Gene_expression (rats) of G3 in spine associated with cancer

6) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 1.17 Pain Relevance 0.04

There appeared to be a trend to a more progressive weight loss pattern in the versican G3 group following tumor cell injection, however, the differences were statistically not significant (p = 0.07).

Gene_expression (group) of G3 associated with weight loss and cancer

7) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 1.12 Pain Relevance 0

The importance of versican G3 with its EGF-like motifs on local tumor invasion has been demonstrated in other cancer cell types [23-25].

Gene_expression (importance) of G3 associated with cancer

8) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 1.55 Pain Relevance 0.03

The propensity of versican G3 to influence tumor invasion to bone and the mechanisms of versican G3 mediated osteolysis warrants ongoing study.

Gene_expression (propensity) of G3 associated with cancer and osteolysis

9) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.90 Pain Relevance 0

Human MT-1 cells stably transfected with the G3 construct or the control vector were cultured at a density of 1 × 105 cells/ml/well on 12-well tissue culture plates in RPMI-1640 media (Life Technologies, Inc.

Gene_expression (transfected) of G3

10) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.14 Pain Relevance 0

In vivo G3 effects on systemic metastasis

Gene_expression (effects) of G3 associated with metastasis

11) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.96 Pain Relevance 0

In vitro effects of G3 on endothelial cells

Gene_expression (effects) of G3 in endothelial cells

12) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.06 Pain Relevance 0

At necroscopy, macroscopic tumor burden in soft tissue metastatic sites was greater for the versican G3 group.

Gene_expression (group) of G3 associated with cancer

13) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 1.12 Pain Relevance 0

In vivo G3 effects on local tumor growth

Gene_expression (effects) of G3 associated with cancer

14) Confidence 0.10 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.35 Pain Relevance 0

To evaluate the formation of vessel-like structures, endothelial cells (2 × 105 cells) were inoculated on six-well tissue culture plates to obtain monolayer cultures, and culture medium was replaced with IMDM that had been pre-incubated (48 h) with vector- or G3 transfected cells and that contained 0.5% or 1% FBS.

Gene_expression (transfected) of G3 in endothelial cells

15) Confidence 0.09 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.43 Pain Relevance 0

When endothelial cultures were treated with medium obtained from G3 transfected cells, the cultures formed vessel-like structures (Figure 3).

Gene_expression (transfected) of G3

16) Confidence 0.09 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.35 Pain Relevance 0

G3 expression in breast cancer cells

Gene_expression (expression) of G3 associated with breast cancer

17) Confidence 0.09 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.21 Pain Relevance 0

The expression of G3 in the cell lysate and culture media of MT-1 cells transfected with the G3 construct containing EGF-like motifs is depicted in Figure 1b and is contrasted to the vector control group.

Gene_expression (expression) of G3

18) Confidence 0.09 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.30 Pain Relevance 0

Athymic rats injected with versican G3 transfected human breast carcinoma cells (MT-1) were observed to demonstrate a greater systemic tumor burden than animals injected with control cells.

Gene_expression (transfected) of G3 associated with cancer and breast cancer

19) Confidence 0.09 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 1.48 Pain Relevance 0

For the cell survival and apoptosis assay, endothelial cells (2 × 105 cells) were seeded on six-well tissue culture plates to obtain monolayer cultures, and culture medium was replaced with 1% FBS/IMDM, which had been pre-incubated (5 days) with vector or G3 transfected cells.

Gene_expression (transfected) of G3 in endothelial cells associated with apoptosis

20) Confidence 0.09 Published 2007 Journal Breast Cancer Res Section Body Doc Link PMC2206723 Disease Relevance 0.16 Pain Relevance 0

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