INT221543

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Context Info
Confidence 0.45
First Reported 2007
Last Reported 2007
Negated 3
Speculated 0
Reported most in Body
Documents 1
Total Number 17
Disease Relevance 1.79
Pain Relevance 0.83

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

lipid binding (Fes) Golgi apparatus (Fes) plasma membrane (Fes)
cytoskeleton (Fes) kinase activity (Fes) cytoplasm (Fes)
Anatomy Link Frequency
brains 3
neurons 2
bands 1
Fes (Mus musculus)
Pain Link Frequency Relevance Heat
dorsal root ganglion 629 100.00 Very High Very High Very High
Root ganglion neuron 17 95.28 Very High Very High Very High
nociceptor 17 81.24 Quite High
withdrawal 17 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Ganglion Cysts 646 100.00 Very High Very High Very High
Shock 374 100.00 Very High Very High Very High
Adhesions 17 38.88 Quite Low
Sprains And Strains 34 32.92 Quite Low
Apoptosis 51 5.00 Very Low Very Low Very Low
Targeted Disruption 51 5.00 Very Low Very Low Very Low
Death 17 5.00 Very Low Very Low Very Low
Wallerian Degeneration 17 5.00 Very Low Very Low Very Low
Urological Neuroanatomy 17 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Similarly, it is possible that the VSV epitope tag on the recombinant PlexinA1 used in our study was permissive of associations that compromised kinase autophosphorylation, but precluded PlexinA1 phosphorylation, and subsequent high affinity associations with Fps or Fer.
Fps Binding (associations) of
1) Confidence 0.45 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.05 Pain Relevance 0.03
This might reveal different specificities of Fps and Fer for interactions with the different PlexinAs.
Fps Binding (interactions) of
2) Confidence 0.45 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.17 Pain Relevance 0.16
Interestingly, Mitsui and coworkers did not detect an association between Fps and CRAM or CRMP in their Cos-7 transfection studies; but they did observe an anti-Fps immunoreactive protein in CRAM immunoprecipitates from rat brains [20].
Fps Neg (not) Binding (association) of in brains
3) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.06 Pain Relevance 0.03
Fps has been shown to associate with microtubules by Takahashi and colleagues, who also observed Fps co-localizing with sites of microtubule nucleation and bundling in transfected Cos-7 cells; furthermore, the observed Fps co-localization with ?
Fps Binding (associate) of
4) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.06 Pain Relevance 0
These results argue that PlexinA1 can interact in some way with Fps and Fer kinases and modify their kinase activities, but not necessarily by acting as a substrate.
Fps Binding (interact) of
5) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
PlexinA1 inhibits the autophosphorylation activities of Fps and Fer
Fps Binding (activities) of
6) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.17 Pain Relevance 0.08
No association between PlexinA1 and Fps or Fer was detected using this method (data not shown).


Fps Neg (No) Binding (association) of
7) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
Interestingly, there is a slightly better conservation with rat Fer than rat Fps in these peptides (19/22 vs 18/22 amino acids).
Fps Binding (conservation) of
8) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.24 Pain Relevance 0.08
To test for associations between CRMP2 and Fps and Fer, this experiment was also performed using the anti-Fps/Fer antibody to isolate the Fps and Fer proteins, followed by immunoblotting with anti-Myc to assess the presence of associated CRMP2 protein.
Fps Binding (associations) of
9) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.21 Pain Relevance 0.07
Purified Fps was also reported to phosphorylate tubulin and promote its polymerization in vitro; and biochemical association between Fps and soluble tubulin was dependent upon the Fps FCH domain [22].
Fps Binding (association) of
10) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
No tyrosine phosphorylated bands corresponding to the size of PlexinA1 were observed in these Myc immunoprecipitates (data not shown), and no association of PlexinA1 with Fps or Fer was found in these co-immunoprecipitation analyses (data not shown).
Fps Neg (no) Binding (association) of in bands
11) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.14 Pain Relevance 0.07
A "pull-down" assay using the GST-PlexinA1 cytoplasmic domain fusion was also performed to further test for possible association between PlexinA1 and Fps or Fer.
Fps Binding (association) of
12) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
Fps was previously identified in a complex with CRMP2 and CRAM in neonatal rat brains; and in co-transfection studies, Fps was shown to phosphorylate all four CRMPs [20].
Fps Binding (complex) of in brains
13) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
The CRAM-Fps association observed in anti-CRAM immunoprecipitates from rat brain lysates by Mitsui and colleagues was presumably a very strong interaction to have survived the biochemical purification steps leading to Fps identification [20].
Fps Binding (association) of in brain
14) Confidence 0.35 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0 Pain Relevance 0
Fps was previously implicated in a Semaphorin signaling pathway in DRG neurons by binding to and phosphorylating both the Semaphorin receptor transducing subunit PlexinA1, and the downstream CRMP-CRAM complex [20].
Fps Binding (binding) of in neurons associated with dorsal root ganglion
15) Confidence 0.33 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.18 Pain Relevance 0.09
Fps/Fes was previously implicated in Semaphorin 3A (Sema3A)-induced growth cone collapse signaling in neurons from the dorsal root ganglion (DRG) through interaction with and phosphorylation of the Sema3A receptor component PlexinA1, and members of the collapsin response mediator protein (CRMP) family of microtubule regulators.
Fps Binding (interaction) of in neurons associated with ganglion cysts, dorsal root ganglion and shock
16) Confidence 0.33 Published 2007 Journal BMC Dev Biol Section Abstract Doc Link PMC2217550 Disease Relevance 0.45 Pain Relevance 0.18
The PlexinA1 construct used by Mitsui and colleagues were C-terminally tagged with the HA epitope; and in Cos-7 cell experiments, this protein was both a substrate and a binding partner for Fps [20].
Fps Binding (binding) of
17) Confidence 0.29 Published 2007 Journal BMC Dev Biol Section Body Doc Link PMC2217550 Disease Relevance 0.07 Pain Relevance 0.03

General Comments

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